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Tsiaj Tag Nrho RNA Kev Rho Tawm
Cov khoom siv siv cov kem ib puag ncig thiab cov mis mos tsim los ntawm Foregene, uas tuaj yeem muab cov khoom muaj txiaj ntsig zoo thiab muaj txiaj ntsig zoo tag nrho RNA los ntawm ntau cov tsiaj nqaij. Nws muab cov DNA-Tu sab uas tuaj yeem yooj yim tshem cov genomic DNA ntawm cov supernatant thiab cov nqaij lysate. RNA nkaus nkaus xwb tuaj yeem cuam tshuam RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Cov kab ke tag nrho tsis muaj RNase, yog li RNA tsis ua haujlwm yuav tsis muaj kev puas tsuaj. Buffer RW1 thiab Buffer RW2 tuaj yeem xyuas kom meej tias RNA tau txais yog tsis muaj kab mob los ntawm cov protein, DNA, ions, thiab cov organic.
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Cell RNA Kev Rho Tawm Rau Cov Khoom Siv
Cov khoom siv no siv cov lus sib dhos thiab cov mis sib xyaw tsim los ntawm Foregene, uas tuaj yeem ua kom zoo-siab thiab muaj txiaj ntsig zoo tag nrho RNA los ntawm cov hlwb tsim hauv 96, 24, 12, thiab 6-daim hlau zoo. Cov khoom siv muab cov DNA kom zoo-Tu Ntu, uas tuaj yeem yooj yim cais cov supernatant thiab lysate ntawm tes, khi thiab tshem cov DNA tseeb. Lub sijhawm ua haujlwm yog yooj yim thiab txuag sijhawm; RNA nkaus xwb tuaj yeem cuam tshuam RNA nrog ib tus qauv tshwj xeeb. Coob tus qauv tuaj yeem ua tib lub sijhawm.
Cov kab ke tag nrho yog RNase-Dawb, kom lub RNA purified tsis muaj kev puas tsuaj; Tsis RW1, Tsis RW2 lub tshuab ntxhua khaub ncaws guaratee qhov tau txais RNA tsis muaj protein, DNA, ion, thiab cov pa phem.
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Viral DNA RNA Cov Khoom Siv Kev Rho Tawm
Cov khoom siv siv cov kem ib puag ncig thiab cov mis tsim los ntawm Foregene, uas muaj peev xwm ua kom tau qhov siab zoo thiab muaj txiaj ntsig zoo DNA thiab RNA los ntawm cov piv txwv xws li ntshav, ntshav dej, lub cev tsis muaj ntshav ntawm lub cev, thiab lub cev ntawm tes supernatant. Cov khoom siv tshwj xeeb ntxiv Linear Acrylamide, uas tuaj yeem yooj yim khaws cov DNA thiab RNA me me los ntawm cov qauv. DNA / RNA-Tsuas Ntu tuaj yeem cuam tshuam DNA thiab RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA lub tshuab ua kom huv yuav tsis muaj qhov puas tsuaj. Tsis RW1 thiab Tsis RW2 tuaj yeem ua kom paub meej tias tau txais cov kis mob nucleic acid tsis muaj protein ntau, nuclease lossis lwm qhov kev ua kom tsis haum, uas tuaj yeem siv ncaj qha rau cov kev sim qis hauv molecular biology.
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Cog Tag Nrho RNA Rho Tawm Ntxiv Cov Khoom Siv
Cov khoom siv siv cov kem ib puag ncig thiab cov mis mos tsim los ntawm Foregene, uas tuaj yeem muab cov khoom zoo siab thiab muaj txiaj ntsig zoo tag nrho RNA los ntawm ntau cov cog ntoo nrog cov polysaccharides lossis polyphenols cov ntsiab lus. Nws muab cov DNA-Tu sab uas tuaj yeem yooj yim tshem cov genomic DNA ntawm cov supernatant thiab cov nqaij lysate. RNA nkaus nkaus xwb tuaj yeem cuam tshuam RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA lub tshuab ua kom huv yuav tsis muaj qhov puas tsuaj. Buffer PRW1 thiab Buffer PRW2 tuaj yeem xyuas kom meej tias RNA tau txais yog tsis muaj kab mob los ntawm cov protein, DNA, ions, thiab cov organic.
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Viral DNA & RNA Cov Khoom Siv Kev Rho Tawm
Cov khoom siv siv cov kem ib puag ncig thiab cov mis tsim los ntawm Foregene, uas muaj peev xwm ua kom tau qhov siab zoo thiab muaj txiaj ntsig zoo DNA thiab RNA los ntawm cov piv txwv xws li ntshav, ntshav dej, lub cev tsis muaj ntshav ntawm lub cev, thiab lub cev ntawm tes supernatant. Cov khoom siv tshwj xeeb ntxiv Linear Acrylamide, uas tuaj yeem yooj yim khaws cov DNA thiab RNA me me los ntawm cov qauv. DNA / RNA-Tsuas Ntu tuaj yeem cuam tshuam DNA thiab RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA lub tshuab ua kom huv yuav tsis muaj qhov puas tsuaj. Tsis RW1 thiab Tsis RW2 tuaj yeem ua kom paub meej tias tau txais cov kis mob nucleic acid tsis muaj protein ntau, nuclease lossis lwm qhov kev ua kom tsis haum, uas tuaj yeem siv ncaj qha rau cov kev sim qis hauv molecular biology.
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Tus Mob RNA Lub Khaub Ncaws RNA
Cov khoom siv siv cov kem ib puag ncig thiab cov mis tsim los ntawm Foregene, uas tuaj yeem ua kom muaj qhov siab thiab huv thiab muaj cov kabmob RNA ntau los ntawm cov piv txwv xws li ntshav, ntshav dej, lub cev tsis muaj lub cev, thiab lub cev ntawm tes supernatant. Cov khoom siv tshwj xeeb ntxiv rau Linear Acrylamide, uas tuaj yeem yooj yim txhom RNA me me los ntawm cov qauv. RNA-Tsuas Ntse tuaj yeem cuam tshuam RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA lub tshuab ua kom huv yuav tsis muaj qhov puas tsuaj. Tsis viRW1 thiab tsis viRW2 tuaj yeem ua kom paub meej tias tau txais cov kis nucleic acid dawb ntawm cov protein, nuclease lossis lwm yam kev cuam tshuam, uas tuaj yeem siv ncaj qha rau cov kev sim ua hauv molecular biology.
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Cog Tag Nrho RNA Rho Tawm Cov Khoom Siv
Cov khoom siv siv cov kem ib puag ncig thiab cov mis mos tsim los ntawm Foregene, uas muaj peev xwm ua kom tau zoo-siab thiab siab zoo tag nrho RNA los ntawm ntau cov cog ntoo nrog cov polysaccharides thiab polyphenols cov ntsiab lus. Rau cov cog ntoo nrog cov polysaccharides siab lossis polyphenols cov ntsiab lus, nws raug nquahu kom siv Cog Tag Nrho RNA Kev Rho Tawm Txuas Ntxiv Khoom kom tau txais cov txiaj ntsig zoo RNA rho tawm. Cov khoom siv muab cov DNA-Tu sab uas tuaj yeem yooj yim tshem cov genomic DNA ntawm cov supernatant thiab lysate nqaij. RNA nkaus nkaus xwb tuaj yeem cuam tshuam RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Cov kab ke tag nrho tsis muaj RNase, yog li RNA tsis ua haujlwm yuav tsis muaj kev puas tsuaj. Buffer PRW1 thiab Buffer PRW2 tuaj yeem xyuas kom meej tias RNA tau txais yog tsis muaj kab mob los ntawm cov protein, DNA, ions, thiab cov organic.
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Cog Tag Nrho RNA Rho Tawm Cov Khoom Siv Ntxiv
Cov khoom siv siv cov kem ib puag ncig thiab cov mis mos tsim los ntawm Foregene, uas tuaj yeem muab cov khoom zoo siab thiab muaj txiaj ntsig zoo tag nrho RNA los ntawm ntau cov cog ntoo nrog cov polysaccharides lossis polyphenols cov ntsiab lus. Nws muab cov DNA-Tu sab uas tuaj yeem yooj yim tshem cov genomic DNA ntawm cov supernatant thiab cov nqaij lysate. RNA nkaus nkaus xwb tuaj yeem cuam tshuam RNA. Cov khoom siv tuaj yeem ua cov txheej txheem ntau ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA lub tshuab ua kom huv yuav tsis muaj qhov puas tsuaj. Buffer PRW1 thiab Buffer PRW2 tuaj yeem xyuas kom meej tias RNA tau txais yog tsis muaj kab mob los ntawm cov protein, DNA, ions, thiab cov organic.
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Cov Khoom Siv Hluav Taws Xob DNA Rho Tawm
Cov khoom siv no siv cov ntawv qhia txog DNA nkaus xwb uas tuaj yeem muab txiav ncaj qha rau DNA, Foregene protease thiab cov cim tsis sib xws, uas yooj yim ntxuav kev ua kom huv ntawm tsob ntoo noob caj noob ces DNA. Muaj cov tshuaj lom neeg zoo DNA tuaj yeem tau txais nyob rau hauv 30 feeb, uas zam kev rhuav tshem ntawm cov DNA ntawm genomic.
Qhov DNA-tsuas yog silica gel membrane siv nyob rau sab kiv yog Foregene cov khoom tshiab tshwj xeeb, uas tuaj yeem ua tau zoo thiab tshwj xeeb khi rau DNA, thiab ua kom zoo tshaj plaws kev tshem tawm RNA, cov tsis muaj zog cov protein, ions, polysaccharides, polyphenols thiab lwm cov organic sib txuas.
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Dav Plasmid Mini Cov Khoom Siv
Cov khoom no txais yuav cov cim DNA nkaus xwb purification kem thev naus laus zis thiab ua kom tau zoo ntawm SDS lysis mis, uas tuaj yeem muab cov plasmid DNA zoo los ntawm cov kab mob hauv 20 feeb.
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Tsiaj Cov Ntaub So Ntshav DNA rho tawm
Cov khoom siv no siv cov kab ntawv DNA nkaus xwb uas tuaj yeem muab txiav ncaj ncaj rau DNA, Foregene protease thiab lub cim tsis zoo. Lub cev muaj txiaj ntsig zoo DNA tuaj yeem muab rho tawm los ntawm ntau lub hlwb thiab cov nqaij tsiaj nyob hauv 30 txog 50 feeb.
Qhov DNA-tsuas yog silica gel membrane siv nyob rau hauv lub kem kiv yog Foregene cov khoom siv tshwj xeeb tshiab, uas tuaj yeem ua tau zoo thiab tshwj xeeb khi rau DNA, thiab ua kom qhov kev tshem tawm RNA ntau tshaj, tsis muaj zog cov protein, ions thiab lwm cov organic sib xyaw hauv cov hlwb. 5-80μg zoo lub genomic DNA tuaj yeem ntxuav los ntawm 10-50mg cov ntaub so ntswg.
