SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Kit Description:
◮ Direct PCR:Tsis xav tau cov khoom siv nucleic acid extaction thiab System, ua tiav 96 tus qauv hauv 1 teev lossis li ntawd.
◮ Cov khoom siv tsawg tsawg thiab siv tau yooj yim heev:Tsuas yog xav tau Real-Time PCR system.
◮ LoD thiab siab rhiab heev:Kev soj ntsuam rhiab heev tuaj yeem ncav cuag qib qis tshaj li 500 daim ntawv / ml.
Npaj siv
Cov khoom siv no siv Real-time RT PCR thev naus laus zis (rRT-PCR) rau kev kuaj pom zoo ntawm SARS-CoV-2 nucleic acids hauv tib neeg nasopharyngeal lossis oropharyngeal swab kuaj.
Txoj cai ntawm kev sim
Cov txheej txheem primer thiab sojntsuam no yog tsim los rau kev tshawb nrhiav cov kev khaws cia ntawm SARS-CoV-2 (ORF1ab gene thiab N gene).Cov khoom siv muaj Internal Control uas yog siv los ntsuam xyuas cov qauv zoo.
Real-Time PCR thev naus laus zis siv cov tshuaj tiv thaiv polymerase saw (PCR) rau kev nthuav dav ntawm lub hom phiaj tshwj xeeb
cov kab ke thiab cov phiaj xwm tshwj xeeb rau kev tshawb pom ntawm RNA amplified.Cov tshuaj ntsuam xyuas tau sau nrog
fluorescent reporter thiab quencher dyes.
Cov ntsiab lus ceev
◮Specifications:48 Rxns/kit, 96 Rxns/kit
◮ Specification:96.72%
rhiab heev:97.92%
◮ Cov hom qauv siv tau
Nasopharyngeal Swab los yog Oropharyngeal Swab
◮Cov cuab yeej siv tau
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Cheebtsam
| Tsis muaj. | Cheebtsam | Tus nqi | Cov khoom tseem ceeb | |||
| 48r xws | 96r xws | |||||
| 1 | Nucleic acid tso tawm tus neeg sawv cev | 1.4ml / tub | 2 cev | 5.3 mL / lub raj mis | 1 fwj | Surfactant |
| 2 | RNA tiv thaiv | 27 μL / tub | 1 cev | 53 μL / tub | 1 cev | RNase inhibitor |
| 3 | SARS-CoV-2 reaction solution | 800 μL / tub | 1 cev | 1600 μL / tub | 1 cev | Primer, sojntsuam, cov tshuaj tiv thaiv tsis zoo, dNTP |
| 4 | SARS-CoV-2 enzyme mix | 80 μL / tub | 1 cev | 160 μL / tub | 1 cev | Kub pib Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 positive control | 100 μL / tub | 1 cev | 100 μL / tub | 1 cev | Recombinant plasmid muaj cov phiaj xwm tawg, RNA |
| 6 | SARS-CoV-2 negative control | 1200 μL / tub | 1 cev | 1200 μL / tub | 1 cev | TE tsis |
Nta & qhov zoo
◮Direct PCR
Tsis xav tau cov khoom siv nucleic acid extaction thiab System, ua tiav 96 tus qauv hauv 1 teev lossis li ntawd.
◮Cov khoom siv tsawg tsawg thiab siv tau yooj yim heev
Tsuas yog xav tau Real-Time PCR system.
◮LoD thiab siab rhiab heev
Kev soj ntsuam rhiab heev tuaj yeem ncav cuag qib qis tshaj li 500 daim ntawv / ml.
Yuav siv li cas
Txoj Kev 1: Direct PCR method
Txoj Kev 2: Viral RNA Isolation + PCR
Txoj Kev 1: Direct PCR txoj kev sivTus Neeg Tso Cai Tso Cai
Kev kuaj xyuas tus txheej txheem
Cold Chain thauj thiab khaws cia
kaw los ntawm lub teeb thiab khaws cia ntawm -20 ± 5 ℃;
Txee lub neej
1 xyoo
Ordering Information
Kev ua haujlwm ceev video
Sau koj cov lus ntawm no thiab xa tuaj rau peb
