SARS-CoV-2 Nucleic Acid Nrhiav Cov Khoom Siv (Multiplex PCR Fluorescent Sojntsuam)

SARS-CoV-2 Nucleic Acid Nrhiav Cov Khoom Siv (Multiplex PCR Fluorescent Sojntsuam)

Cov Khoom Siv Qhia:

Cov khoom siv no siv Real-time RT PCR thev naus laus zis (rRT-PCR) rau kev kuaj pom qhov tseeb ntawm SARS-CoV-2 (ORF1ab noob thiab N caj) nucleic acids nyob rau hauv tib neeg nasopharyngeal lossis oropharyngeal swab sample, los ntawm multiplex PCR fluorescent probe method.Qhov khoom siv no muaj kev tswj hwm sab hauv txhawm rau ntsuas cov kab mob kom zoo, yog li tsis tas yuav muaj nucleic acid purification, tuaj yeem ua tiav kev sim hauv 1 teev, tshwj xeeb tshaj yog tsim nyog rau kev ntsuas loj sai sai.


Khoom Nthuav dav

Cov khoom lag luam

Cov Lus Qhia Tshwj Xeeb

48 Rxns, 96 Rxns

Tsis yog.

Txheej Txheem

Cov nqi

Lub ntsiab tivthaiv

48 Rxns

96 Rxns

1 Nucleic acid tso tus neeg sawv cev 1.4mL / raj 2 raj 5.3 mL / lub raj mis 1 fwj Tub Yaj
2 RNA tiv thaiv 27 μL / raj 1 raj 53 μL / raj 1 raj RNase inhibitor
3 Cov tshuaj tiv thaiv SARS-CoV-2 daws 800 μL / raj 1 raj 1600 μL / raj 1 raj Primer, sojntsuam, qhov tsis haum, dNTP
4 SARS-CoV-2 enzyme sib xyaw 80 μL / raj 1 raj 160 μL / raj 1 raj Kub pib Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 qhov kev tswj tau zoo 100 μL / raj 1 raj 100 μL / raj 1 raj Recombinant plasmid muaj lub hom phiaj thooj, RNA
6 SARS-CoV-2 qhov kev tswj tsis zoo 1200 μL / raj 1 raj 1200 μL / raj 1 raj TE tsis

Nta & qhov zoo

Sai thiab yooj yim.
Txav nrog nucleic acid poob.
Tsis tshua muaj neeg pom qhov kev txwv thiab qhov siab rhiab heev.
Cov cuab yeej siv qis thiab kev siv siab yoog raws siv.

Kev kuaj pom qhov ua tau zoo tuaj yeem ncav qib qis tshaj li 500 daim ntawv / mL).

GCE / mL Tus nqi Ct
N noob ORF1ab noob
2.00E + 08 16.43 15.16
2.00E + 07 19.48 18.58
2.00E + 06 22.71 21,86
2.00E + 05 25.95 25.33
2.00E + 04 29.30 28.49
2.00E + 03 32.37 31.37
2.00E + 02 35.32 34.47
2.00E + 01 38.29 38.27
2.00E + 00 Tsis muaj Tsis muaj
2.00E-01 Tsis muaj Tsis muaj
NC Tsis muaj Tsis muaj

Kev nthuav dav kev nthuav dav

amplification curve-RT-qPCR

Yuav siv li cas

1. Siv cov twj paj nruag

covid-19 nucleic acid detection kit1

2. Kev kuaj mob tus txheej txheem

covid-19 nucleic acid detection kit2

Coj mus kuaj: Naopharyngeal lossis oropharyngeal swab qauv (tsuas yog 1 kauj ruam ua haujlwm) (Tos rau 10 mins)

PCR amplificaton (<= 55min)

Workflow:

covid-19 nucleic acid detection kit4

Cia

kaw los ntawm lub teeb ci thiab khaws cia ntawm -20 ± 5 ℃;

storage
storage2

Lub neej txee: 1 xyoos


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa rau peb