Viral DNA & RNA Isolation Kit Viral DNA thiab RNA Extraction Purification Preparation Kits
Specifications
50 Preps, 200 Preps
Viral RNA Nucleic acid Purification Extraction Isolation Kit siv cov kab rov tav thiab cov qauv tsim los ntawm Foregene, uas tuaj yeem tshem tawm cov purity thiab high-zoo kab mob RNA los ntawm cov qauv xws li ntshav, ntshav, cell-free lub cev kua, thiab cell culture supernatant.Cov khoom siv tshwj xeeb ntxiv Linear Acrylamide, uas tuaj yeem ntes tau me me ntawm RNA los ntawm cov qauv.RNA-Tsuas Kem tuaj yeem khi RNA zoo.Cov khoom siv tuaj yeem ua cov qauv ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA purified yuav tsis degraded.Buffer viRW1 thiab Buffer viRW2 tuaj yeem ua kom ntseeg tau tias cov kab mob kis tau los ntawm nucleic acid tsis muaj protein ntau, nuclease lossis lwm yam impurities, uas tuaj yeem siv ncaj qha rau kev sim hauv dej molecular biology.
Cov khoom siv
| Linear Acrylamide |
| Buffer DRL |
| Buffer RW1, Tsis RW2 |
| RNase-Dawb ddH2O |
| DNA/RNA kab |
| Cov lus qhia |
Nta & qhov zoo
■ Kev khiav hauj lwm ntawm chav tsev kub (15-25 ℃) thoob plaws hauv tag nrho cov txheej txheem, tsis muaj dej khov da dej thiab tsis kub centrifugation.
■ Ua kom tiav cov khoom siv RNase-Free, tsis tas yuav txhawj txog RNA degradation.
■ High nucleic acid tawm los: DNA / RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv DNA thiab RNA zoo.
■ Muaj peev xwm ua tau zoo: txog 200μl cov qauv tuaj yeem ua tiav txhua lub sijhawm.
■ Ceev ceev: yooj yim rau kev khiav lag luam thiab tuaj yeem ua tiav hauv 20 feeb.
■ Kev nyab xeeb: tsis tas yuav muaj cov organic reagent.
■ Zoo: Cov purified RNA tawg yog cov purity siab, tsis muaj protein ntau thiab lwm yam impurities, thiab tuaj yeem ua tau raws li ntau yam kev sim hauv qab.
Daim ntawv thov Kit
Nws yog qhov tsim nyog rau kev tshem tawm thiab ua kom huv ntawm cov kab mob nucleic acid hauv cov qauv xws li ntshav, ntshav, cell-free lub cev kua thiab cell kab lis kev cai supernatant.
Kev khiav haujlwm
Daim duab
Cia thiab Txee lub neej
■ Cov khoom siv no tuaj yeem khaws cia rau 24 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ℃);yog tias nws yuav tsum tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia hauv 2-8 ℃.
■ Cov tshuaj Linear Acrylamide tuaj yeem khaws cia hauv chav sov li 7 hnub;Tom qab tau txais cov khoom siv, thov tshem tawm thiab khaws cia ntawm -20 ° C.
■ Tom qab ntxiv Linear Acrylamide rau Buffer DRL, nws tuaj yeem khaws cia ntawm 2-8 ° C rau txog 48 teev.Thov siv cov tshuaj npaj txhij.
Phau ntawv qhia txog teeb meem
Cov hauv qab no yog kev tshuaj xyuas ntawm cov teeb meem uas yuav ntsib hauv kev rho tawm cov kab mob DNA / RNA, vam tias yuav pab tau rau koj qhov kev sim.Tsis tas li ntawd, rau lwm yam kev sim lossis kev teeb meem uas tsis yog cov lus qhia ua haujlwm thiab kev txheeb xyuas qhov teeb meem, peb tau mob siab rau kev txhawb nqa los pab koj.Yog tias koj muaj kev xav tau, thov hu rau peb: 028-83360257 lossis E-mail:
Tech@foregene.com.
Tsis muaj nucleic acid extraction lossis qis nucleic acid tawm los
Feem ntau muaj ntau yam uas cuam tshuam rau kev rov ua haujlwm zoo, xws li: piv txwv nucleic acid cov ntsiab lus, kev ua haujlwm, elution ntim, thiab lwm yam.
Kev tshuaj xyuas ntawm cov laj thawj uas tshwm sim:
1. Kev da dej dej khov lossis qhov kub qis (4 ° C) centrifugation tau ua thaum lub sijhawm ua haujlwm.
Tswv yim: Ua haujlwm ntawm chav tsev kub (15-25 ° C) thoob plaws hauv tag nrho cov txheej txheem, tsis txhob da dej dej khov thiab tsis kub centrifugation.
2. Cov qauv khaws cia tsis raug lossis cov qauv khaws cia ntev dhau.
Kev pom zoo: Khaws cov qauv ntawm -80 ° C thiab tsis txhob rov khov thiab thawing;sim siv cov qauv sau tshiab rau nucleic acid extraction.
3. Cov qauv lysis tsis txaus.
Cov lus pom zoo: Thov xyuas kom meej tias cov qauv thiab cov kev ua haujlwm lysis tau sib xyaw kom zoo thiab ua rau hauv chav sov (15-25 ° C) rau 10 feeb.
4. Tsis raug ntxiv ntawm eluent.
Tswv yim: Nco ntsoov tias RNase-Free ddH2O yog ntxiv dropwise mus rau nruab nrab ntawm cov purification kem daim nyias nyias, thiab tsis txhob tso rau ntawm lub purification kem nplhaib.
5. Qhov tseeb ntim ntawm ethanol tsis tau ntxiv rau Buffer RW2.
Tswv yim: Thov ua raws li cov lus qhia, ntxiv qhov tseeb ntim ntawm ethanol rau Buffer RW2 thiab sib tov zoo ua ntej siv cov khoom siv.
6. Cov qauv ntim tsis tsim nyog.
Tswv yim: 200µl ntawm cov qauv yog ua tiav rau txhua 500µl ntawm Buffer DRL.Kev ua piv txwv ntau dhau yuav ua rau qis dua nucleic acid extraction tawm los.
7. Tsis tsim nyog elution ntim lossis tsis tiav elution.
Kev pom zoo: Lub ntim eluent ntawm purification kem yog 30-50μl;Yog tias cov nyhuv elution tsis txaus siab, nws raug nquahu kom ncua lub sijhawm ntawm chav tsev kub tom qab ntxiv preheated RNase-Free ddH2O, xws li 5-10min.
8. Ethanol tseem nyob ntawm kem tom qab ntxuav nrog Buffer RW2.
Cov lus qhia: Yog tias ethanol tseem tshuav tom qab centrifugation nrog Buffer RW2 rau 2 feeb, kem tuaj yeem muab tso rau hauv chav sov li 5 feeb tom qab centrifugation kom tshem tawm cov ethanol seem.
Lub purified nucleic acid yog degraded
Qhov zoo ntawm cov purified nucleic acid muaj feem xyuam rau kev khaws cia ntawm cov qauv, RNase paug, kev ua haujlwm thiab lwm yam.Kev tshuaj xyuas ntawm cov laj thawj uas tshwm sim:
1. Cov qauv sau tseg tsis tau khaws cia rau lub sijhawm.
Cov lus qhia: Yog tias tus qauv tsis siv sijhawm tom qab sau, thov khaws cia ntawm -80 ° C ntawm qhov kub qis tam sim ntawd.Rau RNA rho tawm, sim siv cov qauv sau tshiab.
2. Sau cov qauv thiab khov thiab thaw ntau zaus.
Tswv yim: Tsis txhob khov thiab thawing (tsis ntau tshaj ib zaug) thaum lub sij hawm sau thiab cia cov qauv, txwv tsis pub cov nucleic acid tawm los yuav raug txo.
3. RNase tau qhia hauv chav ua haujlwm lossis cov hnab looj tes pov tseg, qhov ncauj qhov ntswg, thiab lwm yam tsis hnav.
Kev pom zoo: RNA extraction thwmsim tau ua tau zoo tshaj plaws hauv chav ua haujlwm RNA cais, thiab chav kuaj yuav tsum tau ntxuav ua ntej kev sim.
Hnav hnab looj tes thiab qhov ncauj qhov ntswg pov tseg thaum lub sij hawm sim kom tsis txhob RNA degradation los ntawm kev qhia txog RNase mus rau qhov loj tshaj plaws.
4. Cov reagent tau kis nrog RNase thaum siv.
Daim Ntawv Pom Zoo: Hloov nrog Viral DNA / RNA Cov Khoom Siv cais tawm tshiab rau cov kev sim cuam tshuam.
5. Cov raj centrifuge thiab cov lus qhia pipette siv rau RNA manipulation yog kis nrog RNase.
Cov lus qhia: Xyuas kom tseeb tias cov raj centrifuge, cov lus qhia pipette, pipettes, thiab lwm yam siv rau RNA extraction yog tag nrho cov RNase-Free.
Purified nucleic acid cuam tshuam cov kev sim hauv qab
DNA thiab RNA purified los ntawm purification kem, yog hais tias cov ntsev ion thiab protein cov ntsiab lus siab dhau lawm, nws yuav cuam tshuam rau cov kev sim nram qab no, xws li: PCR amplification, thim rov qab, thiab lwm yam.
1. Cov eluted DNA thiab RNA muaj residual ntsev ions.
Tswv yim: Xyuas kom tseeb tias qhov tseeb ntim ntawm cov ethanol yog ntxiv rau Buffer RW2, thiab ntxuav cov purification kem ob zaug ntawm qhov ceev centrifugation teev nyob rau hauv cov lus qhia khiav hauj lwm;Ua centrifugation kom txo qis ntsev ion paug.
2. Cov eluted DNA thiab RNA muaj ethanol residues.
Cov lus qhia: Tom qab paub meej tias kev ntxuav nrog Buffer RW2, ua cov raj khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob RW2.Yog tias tseem muaj ethanol residue, koj tuaj yeem centrifuge lub raj khoob thiab muab tso rau hauv chav sov li 5 feeb kom tshem tawm cov ethanol residue mus rau qhov loj tshaj plaws.
Phau ntawv qhia:
Viral DNA & RNA Isolation Kit Instruction Manual
