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Zebra Ntses Ntsig PCR Khoom Siv
Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm zebrafish thiab lwm cov ntses dej ntses tshiab, ntses fins lossis cov qe ntses coj mus kuaj rau PCR cov tshuaj tiv thaiv, yog li nws tsim tshwj xeeb rau kev kuaj caj ces loj. Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65 ° C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2 × PCR EasyTM Mix muaj kev tiv thaiv zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj mus kuaj raws li tus qauv tsim kom tau txais txiaj ntsig thiab tshwj xeeb. Qhov reagent no muaj ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis haum, PCR optimizer thiab stabilizer. Siv ua ke nrog cov lysis tsis tuaj yeem ua kom sai thiab yooj yim kuaj cov qauv, thiab muaj cov yam ntxwv ntawm kev rhiab siab, muaj zog tshwj xeeb, thiab muaj kev ruaj khov.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
