Raws cov khoom siv cog nplooj Direct PCR Plus, dUTP yog siv hloov ntawm dTTP, thiab UNG enzyme uas tuaj yeem thuam cov qauv uas muaj dUTP ntxiv. Nyob rau hauv txoj kev no, qhov tshwj xeeb thiab qhov tseeb ntawm amplification yog ua kom ntseeg tau, thiab cov teeb meem ntawm PCR cov khoom sib kis uas yuav tshwm sim thaum kuaj cov caj ces loj.