Cell Tag Nrho RNA Isolation Kit Total RNA Isolation Purification Kits los ntawm cell
Kit Description:
Ua kom huv thiab zoo tag nrho RNA tuaj yeem tau txais los ntawm ntau lub hlwb hauv 11 feeb.
RNase-dawb
Ua haujlwm ntawm chav tsev kub (15-25 ℃)
Nrog DNA-Tshuaj Kem
High RNA tawm los
Ceev: Ua kom tiav hauv 11 feeb
Kev nyab xeeb: Tsis muaj tshuaj lom neeg
Kev piav qhia
Cov khoom siv no siv cov kab rov tav thiab cov qauv tsim los ntawm Foregene, uas tuaj yeem tshem tawm cov purity siab thiab siab zoo tag nrho RNA los ntawm cov hlwb kab lis kev cai hauv 96, 24, 12, thiab 6-zoo daim hlau.
Cov khoom siv muab qhov zoo DNA-Tshuaj Kem, uas tuaj yeem yooj yim cais cov supernatant thiab cell lysate, khi thiab tshem tawm genomic DNA.Kev ua haujlwm yog yooj yim thiab txuag lub sijhawm.
Tnws RNA-tsuas yog Kem tuaj yeem khi RNA zoo nrog cov qauv tshwj xeeb.Ntau tus qauv tuaj yeem ua tiav ib txhij.
Cov khoom siv
| Cov khoom siv muaj pes tsawg leeg | TIAB SA-03111 | TIAB SA-03114 |
| 50 T | 200 T | |
| Tsis pub cRL1 * | 25 ml ib | 100 mL |
| Tsis pub cRL2 | 15 ml ib | 60 ml ib |
| Buffer RW1* | 25 ml ib | 100 mL |
| Buffer RW2 | 24ml ib | 96ml ib |
| RNase-Dawb ddH2O | 10 ml ib | 40 ml ib |
| RNA-Tsuas Kem | 50 | 200 |
| DNA-Tswj Kem | 50 | 200 |
| Kev qhia | 1 | 1 |
* Thov hnav hnab looj tes thiab siv kev tiv thaiv thaum lub sijhawm ua haujlwm raws li Buffer cRL1 thiab Buffer RW1 muaj cov ntsev chaotropic irritating.
Nta & qhov zoo
■ Tag nrho cov txheej txheem yog ua nyob rau hauv chav tsev kub (15-25 ℃), tsis muaj dej khov da dej thiab tsis kub centrifugation.
■ Cov khoom siv tag nrho yog RNase-Free, tsis tas yuav txhawj txog RNA degradation.
■ DNA-Tshuaj Kem tshwj xeeb khi DNA, kom cov khoom siv tuaj yeem tshem tawm cov kab mob DNA sib kis tsis tau ntxiv DNase ntxiv.
■ High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv huv RNA zoo.
■ Ceev ceev: yooj yim rau kev khiav lag luam thiab tuaj yeem ua tiav hauv 11 feeb.
■ Kev Nyab Xeeb: Tsis tas yuav tsum muaj cov organic reagent.
■ Zoo: RNA purified yog purity siab, tsis muaj protein ntau thiab lwm yam impurities, thiab tuaj yeem ua tau raws li ntau yam kev sim tom qab.
Daim ntawv thov Kit
Nws tsim nyog rau kev rho tawm thiab ua kom huv ntawm tag nrho RNA los ntawm kab lis kev cai hauv 96, 24, 12, thiab 6-zoo daim hlau.
Kev khiav haujlwm
Daim duab
Lub agarose gel roj teeb daim duab ntawm Cell Tag Nrho RNA Isolation Kit kho cov lej sib txawv ntawm cov hlwb, 20μl ntim elution, noj 2μl purified tag nrho RNA 1%.
Cia thiab txee lub neej
Cov khoom siv tuaj yeem khaws cia rau 12 lub hlis ntawm chav tsev kub (15-25 ℃) lossis 2-8 ℃ rau lub sijhawm ntev (24 lub hlis).
Tsis pub cRL1 tuaj yeem khaws cia ntawm 4 ℃ rau 1 lub hlis tom qab ntxiv 2-hydroxy-1-ethanethiol (optional).
RNA tsis raug rho tawm lossis RNA tawm los tsawg
Feem ntau muaj ntau yam uas cuam tshuam rau kev rov qab ua haujlwm zoo, xws li: cov ntaub so ntswg qauv RNA cov ntsiab lus, kev ua haujlwm, elution ntim, thiab lwm yam.
1. Cov dej khov da dej lossis cryogenic (4 ° C) centrifugation tau ua thaum lub sijhawm ua haujlwm.
Kev pom zoo: Ua haujlwm ntawm chav tsev kub (15-25 ° C) thoob plaws hauv tag nrho cov txheej txheem, tsis txhob dej khov thiab centrifuge ntawm qhov kub qis.
2. Cov qauv khaws cia tsis raug lossis ntau lub sijhawm khaws cia.
Kev pom zoo: Khaws cov qauv ntawm -80 ° C lossis khov hauv cov kua nitrogen thiab tsis txhob rov siv dua khov-thaw;sim siv cov ntaub so ntswg tshiab los yog kab lis kev cai rau RNA rho tawm.
3. Cov qauv lysis tsis txaus.
Daim ntawv pom zoo: Thaum homogenizing cov ntaub so ntswg, xyuas kom meej tias cov ntaub so ntswg txaus homogenized thiab cov ntaub so ntswg hlwb txaus txaus los piav qhia txog kev tso tawm RNA.
4. Lub eluent tsis ntxiv kom raug.
Kev Pom Zoo: Paub meej tias RNase-Free ddH2O yog ntxiv dropwise mus rau nruab nrab ntawm lub purification kem membrane.
5. Qhov tseeb ntim ntawm ethanol tsis tau ntxiv rau Buffer RL2 lossis Buffer RW2.
Kev pom zoo: Ua raws li cov lus qhia, ntxiv qhov tseeb ntim ntawm ethanol rau Buffer RL2 thiab Buffer RW2 thiab sib tov zoo ua ntej siv cov khoom siv.
6. Kev siv cov ntaub so ntswg tsis tsim nyog.
Kev pom zoo: Siv 10-20 mg ntawm cov ntaub so ntswg los yog (1-5) × 106hlwb ib 500 μl tsis RL1, raws li kev siv cov ntaub so ntswg ntau tuaj yeem ua rau txo qis RNA rho tawm.
7. Tsis tsim nyog elution ntim lossis tsis tiav elution.
Kev pom zoo: Lub elution ntim ntawm purification kem yog 50-200 μl;Yog tias cov nyhuv elution tsis txaus siab, nws raug nquahu kom txuas ntxiv chav tsev kub tso sijhawm tom qab ntxiv preheated RNase-Free ddH2O, piv txwv li rau 5-10 min.
8.Cov purification kem muaj ethanol residue tom qab Buffer RW2 ntxuav.
Kev pom zoo: Yog tias muaj ethanol residue tom qab Buffer RW2 ntxuav, khoob raj centrifugation rau 1min, lub sij hawm rau lub raj khoob centrifugation ua hauj lwm yuav nce mus rau 2min, los yog purification kem yuav muab tso rau hauv chav tsev kub rau 5 min kom txaus tshem tawm cov seem ethanol.
Purified RNA yog degraded
Qhov zoo ntawm RNA purified yog muaj feem xyuam rau tej yam xws li kev khaws cia ntawm tus qauv, RNase contamination, thiab manipulation, thiab lwm yam.
1. Cov qauv ntaub so ntswg tsis khaws cia rau lub sijhawm.
Kev pom zoo: Yog tias tsis siv cov ntaub so ntswg los yog cov hlwb tsis siv raws sij hawm tom qab sau, tam sim ntawd cryopreserve ntawm -80 ° C los yog kua nitrogen.Txhawm rau rho tawm RNA, siv cov ntaub so ntswg tshiab lossis cov qauv ntawm tes thaum twg los tau.
2. Rov qab khov-thawing ntawm cov ntaub so ntswg kuaj.
Kev pom zoo: Thaum khaws cov ntaub so ntswg, nws yog qhov zoo tshaj plaws los txiav lawv rau hauv me me rau kev khaws cia, thiab tshem tawm ib qho ntawm cov khoom thaum siv lawv kom tsis txhob rov khov-thawing ntawm cov qauv thiab degradation ntawm RNA.
3. RNase yog qhia los yog tsis hnav hnab looj tes, qhov ncauj qhov ntswg, thiab lwm yam thaum lub sijhawm ua haujlwm.
Kev pom zoo: RNA extraction thwmsim yog ua tau zoo tshaj plaws nyob rau hauv cais RNA manipulation chav thiab lub rooj yog cleared ua ntej qhov kev sim.
Hnav cov hnab looj tes thiab lub qhov ncauj qhov ntswg pov tseg thaum lub sijhawm sim ua kom txo qis RNA degradation los ntawm kev qhia txog RNase.
4. Cov tshuaj tiv thaiv kab mob muaj RNase thaum siv.
Kev Pom Zoo: Hloov nrog Cov Tsiaj Tag Nrho Tag Nrho RNA cais tawm tshiab rau cov kev sim cuam tshuam.
5. Cov raj centrifuge, cov lus qhia, thiab lwm yam siv hauv RNA manipulation yog paug nrog RNase.
Kev pom zoo: Paub meej tias cov raj centrifuge, cov lus qhia, pipettes, thiab lwm yam siv hauv RNA extraction yog txhua yam RNase-Free.
Purified tau txais RNA cuam tshuam cov kev sim hauv qab
RNA purified los ntawm purification kem, yog cov ntsev ions, cov ntsiab lus ntawm cov protein ntau dhau lawm yuav cuam tshuam rau cov kev sim qis qis, xws li: thim rov qab, Northern Blot li al.
1. Cov elutioned RNA muaj ntsev ion residues.
Kev pom zoo: Paub meej tias qhov tseeb ntim ntawm ethanol tau ntxiv rau Buffer RW2 thiab ua 2 purification kem ntxuav ntawm qhov ceev centrifugal qhia rau kev ua haujlwm;yog tias muaj cov ntsev ion residue, tawm hauv kab purification mus rau Buffer RW2 rau 5 min ntawm chav tsev kub thiab ua centrifugation kom loj tshaj qhov kev tshem tawm cov ntsev paug.
2. Ethanol residue nyob rau hauv elutioned RNA.
Cov Lus Taw Qhia: Paub meej tias tom qab tsis yog RW2 ntxhua khaub ncaws ua haujlwm ntawm Centrifugation ceev qhia rau ntawm Centrifugation ceev tseg rau 5 m
