Direct PCR yog dab tsi

Direct PCR yog txoj kev ntawm DNA amplification ncaj qha los ntawmntshav, tsiaj cov ntaub so ntswg,rat qab,cell, pob ntseg,zebra ntses, ntses qe,nplooj nplooj,nooblos yog lwm yam qauv ntawm cov ntaub so ntswg uas tsis muaj DNA cais thiab ua kom huv si.Cov txheej txheem PCR ncaj qha tuaj yeem txo lub sijhawm sim, thiab tus nqi hauv genotyping thiab ntau qhov project.Nws kuj tseem muab cov kev xaiv zoo dua thaum ntsib cov teeb meem ntawm kev nthuav dav me me ntawm cov qauv uas cov kauj ruam ua kom huv tuaj yeem ua rau cov qauv poob.

FOREGENE's PCR Mix muaj zog tiv thaiv kev ntxhov siab, vim FOREGENE lub zog patented Taq enzyme (tso cai patent: ZL20161034512.0 (Tuam Tshoj), EP3450560 (Tebchaws Europe), PCT/CN2017/082154 (US), patent 68Jap49).Nws Taq enzyme siv DNA recombination thev naus laus zis los ua kom cov DNA khi cov qauv hauv cheeb tsam ntawm archaeal nucleic acid binding protein nrog cov tshav kub-resistant Taq DNA polymerase los tsim cov qauv DNA nrog kev sib txuas ntxiv.Ib qho kev txhim kho kub-pib Taq uas khaws ntau yam haujlwm ntawm thawj DNA polymerase.This Taq enzyme tuaj yeem khi rau cov qauv DNA thiab pib DNA synthesis hauv cov qauv qis qis thiab cov cheeb tsam nyuaj.

For piv txwv,ityog yooj yim li ntxiv kojcell,nroj tsuag (cov nplooj hluas, cag los yog noob) los yog tsiaj coj mus rau lubtsis, thiab ntxiv qhov tshwj xeebmaster mix nrogtshwj xeebprimers nyob rau hauv PCR raj thiab khiav nws los ntawm ib tug thermal cycler.Cov khoom siv tau zoo yog qhov zoo tagnrho rau cov qauv ntsuas loj uas txuag lub sijhawm tseem ceeb heev.

PCR cov tshuaj tiv thaiv uas muaj UNG enzyme thiab dUTP raug xaiv raws li cov kev xav tau ntawm kev sim, uas tuaj yeem zam tau cov pa phem los ntawm PCR amplification khoom.