Xov xwm muaj
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Ua tib zoo mloog cov ntsiab lus hauv qab no kom ua tiav ob npaug ntawm koj qhov kev sim RT-qPCR!
RT-qPCR yog qhov kev sim yooj yim ntawm molecular biology, thiab txhua tus yuav tsum paub txog nws.Nws feem ntau suav nrog peb kauj ruam: RNA rho tawm, thim rov qab rau hauv cDNA, thiab lub sijhawm fluorescent quantitative PCR.Nws tsis pab, dab tsi tshwm sim?Zoo li yuav muaj teeb meem w...Nyeem ntxiv -
RNA rho tawm, 260/230 tshwj xeeb yog qis, thiab tus nqi ct ntawm pcr khiav yog siab heev.Yuav daws li cas?
Qhov qis piv ntawm A260 / A230 feem ntau yog tshwm sim los ntawm impurities nrog qhov siab tshaj plaws nqus wavelength ntawm 230nm.Cia peb pom dab tsi cov impurities muaj xws li: Cov pa phem feem ntau Absorption wavelength Ratio effect Protein ~ 230nm thiab 280nm Ib txhij txo ntawm A 260 / A 280 thiab A 260 / A ...Nyeem ntxiv -
Fluorescent quantitative PCR (qPCR) - tus tsim primer
Hauv kev sim qPCR, primer tsim kuj yog qhov txuas tseem ceeb heev.Txawm hais tias cov primers tsim nyog los yog tsis yog ze ze rau seb qhov kev ua kom pom kev ua tau zoo nce mus txog tus qauv, seb cov khoom lag luam amplified yog qhov tshwj xeeb, thiab seb qhov kev sim tau muaj.Yog li yuav ua li cas ...Nyeem ntxiv -
Cov tshuaj ntxiv hauv PCR cov tshuaj tiv thaiv thiab lawv cov haujlwm
Kuv ntseeg tias txhua tus neeg yuav ib txwm ntsib cov teeb meem zoo li no lossis cov teeb meem zoo li no thaum ua PCR cov tshuaj tiv thaiv, tab sis feem ntau ntawm lawv tuaj yeem muab faib ua ob qhov teeb meem tseem ceeb: Kev nthuav dav me me ntawm cov qauv qauv (amplification);Ntau dhau tsis yog hom phiaj gene amplification.Kev siv cov additives yog ib qho ntawm cov stra...Nyeem ntxiv -
Cov ntsiab lus ntawm 45 gene therapy tshuaj uas tau muag thoob ntiaj teb
Cov noob caj noob ces yog cov caj ces tseem ceeb uas tswj cov yam ntxwv.Tsuas yog cov noob ntawm qee cov kab mob, uas yog tsim los ntawm RNA, cov noob ntawm cov kab mob feem ntau yog tsim los ntawm DNA.Feem ntau cov kab mob ntawm cov kab mob yog tshwm sim los ntawm kev sib cuam tshuam ntawm cov noob thiab ib puag ncig.Gene therapy tuaj yeem kho tau o ...Nyeem ntxiv -
Nyob rau hauv lub era ntawm kev kho RNA, leej twg tuaj yeem dhau los ua "tus hlub tshiab" ntawm kev lag luam |Cov khoom muag txhua xyoo
Tau qhov twg los: WuXi AppTec Tsis ntev los no, thaj chaw ntawm RNA txoj kev kho tau pom qhov sib txawv - hauv 5 lub xyoo dhau los ib leeg, 11 RNA kev kho mob tau pom zoo los ntawm FDA, thiab tus lej no tseem tshaj li cov txiaj ntsig ntawm kev kho RNA yav dhau los!Piv nrog rau cov kev kho mob ib txwm muaj, RNA therapy ca ...Nyeem ntxiv -
Ordinary PCR, fluorescent PCR, digital PCR;tsom xam ntawm qhov zoo thiab qhov tsis zoo!
Ntau qhov kev hloov pauv hloov pauv hauv keeb kwm ntawm kev tshawb nrhiav thev naus laus zis hauv kuv lub siab yog immunolabeling thev naus laus zis raws li lub hauv paus ntsiab lus ntawm antigen-antibody tshwj xeeb khi, PCR technology thiab sequencing technology.Niaj hnub no peb yuav tham txog PCR technology.Raws li t ...Nyeem ntxiv -
Cov ntsiab lus | Raws li lub Yim Hli 2022, 41 cov tshuaj kho noob tau raug tsim tawm thoob ntiaj teb.
Tsis ntev los no, peb cov tshuaj kho noob tau raug pom zoo rau kev lag luam, xws li: (1) Thaum Lub Xya Hli 21, 2022, PTC Therapeutics, Inc. (NASDAQ: PTCT) tshaj tawm tias nws AAV gene therapy Upstaza™ tau pom zoo los ntawm European Commission Nws yog thawj cov tshuaj kho noob muag ncaj qha hauv ...Nyeem ntxiv -
Yav dhau los thiab tam sim no ntawm CACLP
Los ntawm Lub Kaum Hli 26th txog 28th, 2022, lub koob tsheej tshiab ntawm kev sim tshuaj thiab kev kuaj mob hauv vitro - Lub 19th Tuam Tshoj Tuam Tshoj Association of Clinical Laboratory Practice Expo Lub 2nd China IVD Supply Chain Expo (CISCE) tau ua tiav ntawm Nanchang Greenland International Expo Center.FOREGEN CO...Nyeem ntxiv -
PCR tshuab | Koj puas to taub tiag?
PCR tshuab | Koj puas to taub tiag?Nobel Prize-winning PCR thev naus laus zis Hauv xyoo 1993, tus kws tshawb fawb Asmeskas Mulis tau txais Nobel nqi zog hauv Chemistry, thiab nws qhov kev ua tiav yog qhov tsim ntawm PCR thev naus laus zis.Cov khawv koob ntawm PCR thev naus laus zis yog nyob rau hauv cov yam ntxwv hauv qab no: Ua ntej, tus nqi ntawm DNA rau ...Nyeem ntxiv -
Tseeb Xwm Thesis: Txheeb xyuas cov qauv thiab txiav DNA mechanism ntawm cov noob kho tshiab ISRB
Nyob rau hauv kaum xyoo dhau los, cov noob kho cov thev naus laus zis raws li CRISPR tau tsim kho sai, thiab tau ua tiav zoo rau kev kho cov kab mob caj ces thiab mob qog noj ntshav hauv tib neeg kev sim tshuaj.Nyob rau tib lub sijhawm, cov kws tshawb fawb thoob ntiaj teb niaj hnub siv cov cuab yeej tshiab tshiab nrog cov noob hloov kho po...Nyeem ntxiv -
Gene therapy, kiag li "sawv" pob ntseg
Kev hnov lus (HL) yog kab mob uas muaj feem cuam tshuam rau tib neeg.Hauv cov teb chaws tsim kho, kwv yees li 80% ntawm cov neeg lag ntseg ua ntej ua ntej ntawm cov menyuam yaus yog tshwm sim los ntawm caj ces.Feem ntau yog cov noob tsis xws luag (raws li qhia hauv daim duab 1), 124 noob hloov tau pom tias muaj feem cuam tshuam nrog wi ...Nyeem ntxiv
