• facebook
  • linkedin
  • youtube
paj_banner

Plant Total RNA Isolation Kit Total RNA Purificaiton Kit rau Cog Tsawg hauv Polysaccharides thiab Polyphenols

Kit Description:

Cat. No. RE-05011/05014

Rau kev ua kom huv ntawm tag nrho RNA los ntawm cov qauv cog dav dav uas muaj cov polysaccharide tsawg thiab polyphenol Cheebtsam.

Ceev nrooj tshem tawm tag nrho cov RNA zoo los ntawm cov qauv cog nrog cov ntsiab lus tsis tshua muaj polysaccharide thiab polyphenol.

RNase-dawb

Kev tshem tawm DNA zoo siv DNA-Tshuaj Kem

Tshem tawm DNA yam tsis tau ntxiv DNase

Yooj yim-tag nrho cov haujlwm ua tiav ntawm chav tsev kub

Fast-kev ua haujlwm tuaj yeem ua tiav hauv 30 feeb

Kev nyab xeeb - tsis muaj cov organic reagent siv


Product Detail

Khoom cim npe

FAQ

DOWNLOAD KHOOM

Specifications

50 Preps, 200 Preps

Cov khoom siv siv cov kab sib dhos thiab cov qauv tsim los ntawm Foregene, uas tuaj yeem ua kom zoo tshaj plaws ntawm cov purity thiab siab zoo tag nrho RNA los ntawm ntau cov ntaub so ntswg nrog cov ntsiab lus polysaccharides thiab polyphenols tsawg.Rau cov qauv cog nrog siab polysaccharides lossis polyphenols cov ntsiab lus, nws raug nquahu kom siv Cov Khoom Siv Cog Tag Nrho RNA Isolation Plus kom tau txais txiaj ntsig zoo ntawm RNA extraction.Cov khoom siv muab cov DNA-Tsev kem uas tuaj yeem tshem tawm cov genomic DNA los ntawm supernatant thiab cov ntaub so ntswg lysate.RNA nkaus xwb tuaj yeem khi RNA zoo.Cov khoom siv tuaj yeem ua cov qauv ntau ntawm tib lub sijhawm.

Tag nrho cov kab ke tsis muaj RNase, yog li RNA purified yuav tsis degraded.Buffer PRW1 thiab Buffer PRW2 tuaj yeem ua kom ntseeg tau tias RNA tau txais tsis muaj kab mob los ntawm cov protein, DNA, ions, thiab cov organic tebchaw.

Cov khoom siv

Tsis PSL1, Tsis PS, Tsis PSL2

Buffer PRW1, Buffer PRW2

RNase-Dawb ddH2O, DNA-Tswj Kem

RNA-Tsuas Kem

Cov lus qhia

Nta & qhov zoo

■ Kev khiav hauj lwm ntawm chav tsev kub (15-25 ℃) thoob plaws hauv tag nrho cov txheej txheem, tsis muaj dej khov da dej thiab tsis kub centrifugation.
■ Ua kom tiav cov khoom siv RNase-Free, tsis tas yuav txhawj txog RNA degradation.
■ DNA-Cleaning Kem tshwj xeeb khi rau DNA, yog li cov khoom siv tuaj yeem tshem tawm cov kab mob DNA genomic yam tsis ntxiv DNase.
■ High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv huv RNA zoo.
■ Ceev ceev: yooj yim rau kev khiav lag luam thiab tuaj yeem ua tiav hauv 30 feeb.
■ Kev nyab xeeb: tsis tas yuav muaj cov organic reagent.
■ Zoo: Cov purified RNA tawg yog cov purity siab, tsis muaj protein ntau thiab lwm yam impurities, thiab tuaj yeem ua tau raws li ntau yam kev sim hauv qab.

123

Daim ntawv thov Kit

Nws yog qhov tsim nyog rau kev tshem tawm thiab ua kom huv ntawm tag nrho RNA los ntawm cov qauv tshiab los yog khov cog cov ntaub so ntswg (tshwj xeeb tshaj yog cov nplooj ntoo tshiab) nrog cov ntsiab lus tsis tshua muaj polysaccharide thiab polyphenol.

Kev khiav haujlwm

cog tag nrho RNA-kev ua haujlwm yooj yim

Daim duab

Plant Total RNA Isolation Kit6

Plant Total RNA Isolation Kit Plus ua tiav 50mg ntawm nplooj tshiab ntawm polysaccharides thiab polyphenols, thiab 5% purified RNA raug kuaj los ntawm electrophoresis.
1: Banana
2: Ginkgo
3: paj rwb
4: Pomegranate

Cia thiab txee lub neej

Cov khoom siv tuaj yeem khaws cia rau 12 lub hlis ntawm chav tsev kub (15-25 ℃) hauv qhov chaw qhuav, thiab 2-8 ℃ rau lub sijhawm ntev (24 lub hlis).

Tsis PSL1 tuaj yeem khaws cia ntawm 4 ℃ rau 1 lub hlis tom qab ntxiv 2-hydroxy-1-ethanethiol (yeem).


  • Yav dhau los:
  • Tom ntej:

  • Phau ntawv qhia txog teeb meem

    Cov nram qab no tsom xam ntawm cov teeb meem koj yuav ntsib nyob rau hauvCog tag nrhoRNA extraction will help you with your experiments. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali : Tech@foregene.com.

    Kab rov tav yog clogged

    Kev thaiv ntawm kab rov tav yuav ua rau RNA tawm los txo qis lossis txawm tias tsis tuaj yeem ua kom huv kom tau RNA, thiab qhov zoo ntawm RNA tau txais yuav qis.

    Common Cause Analysis:

    1. Cov qauv tsis tawg tag.

    Cov qauv tsis tiav kev tawg tuaj yeem thaiv DNA-Tshuaj Kem, uas tuaj yeem cuam tshuam rau RNA yield thiab zoo.Peb pom zoo tias thaum ua cov qauv fragmentation, ceev nrooj zom cov kua nitrogen txaus kom tawg cov ntaub so ntswg xws li cov phab ntsa ntawm tes thiab cell membranes ntawm cov qauv kom ntau li ntau tau.Rau cov qauv cog ntawm polyphenol polysaccharides, peb xav kom koj siv Plant Total RNA Isolation Kit Plus.

    2.Aspirate lub DNA-Tshuaj Kem cais cov supernatant, aspirate lub cell debris pellet.

    Aspirated cell debris pellet tuaj yeem txhaws RNA nkaus xwb Kem thaum lub sij hawm RNA adsorption cov txheej txheem (saib kauj ruam 5 ntawm cov txheej txheem, kauj ruam 6 ntawm cov txheej txheem polysaccharide polyphenol).Peb pom zoo tias yuav tsum tau saib xyuas thaum aspirating no supernatant kom tsis txhob muaj cov khib nyiab ntawm tes raug aspirated.

    3. Thawj tus nqi ntawm cov qauv yog loj heev.

    Kev siv cov qauv ntau dhau yuav ua rau tsis tiav cov qauv tawg lossis tsis tiav lysis ntawm cov hlwb los ntawm Buffer PRL1 lossis Buffer PSL1, uas ua rau muaj cov kab purification uas txhaws rau kev ua haujlwm huv.Plant Total RNA Isolation Kit muaj qhov pib siab tshaj plaws ntawm 50 mg rau ib zaug purification ntawm ib qho qauv ua haujlwm.Rau cov qauv cog ntawm polyphenol polysaccharides, peb xav kom koj sim cog tag nrho RNA Isolation Kit Plus.

    4. Qhov kub ntawm lub centrifuge yog qis heev.

    Tag nrho RNA rho tawm thiab ua kom huv tsuas yog ua kua nitrogen cuam tshuam ntawm cov qauv ntaub so ntswg, tag nrho cov kauj ruam yog ua nyob rau hauv chav tsev kub (20-25 ° C).Qee cov centrifuges uas tsis tshua muaj kub muaj qhov kub thiab txias qis dua 20 ° C, uas tuaj yeem ua rau muaj kev cuam tshuam hauv DNA-Tshuaj Kem thiab / lossis RNA nkaus xwb.Yog tias qhov no tshwm sim, teem lub centrifuge kub rau 20-25 ° C thiab ua ntej sov lub lysis sib tov thiab / los yog ntxiv ntawm ethanol sib cais supernatant mus rau 37 ° C.

    Tsis muaj RNA rho tawm lossis RNA tawm los tsawg

    Feem ntau muaj ntau yam uas cuam tshuam rau kev rov ua haujlwm zoo, xws li: qauv RNA cov ntsiab lus, txoj kev ua haujlwm, qhov ntim elution, thiab lwm yam.

    Kev tshuaj xyuas ntawm cov laj thawj uas tshwm sim hauv qab no:

    1.Ib da dej dej khov lossis kub qis (4 ° C) centrifugation tau ua thaum lub sijhawm ua haujlwm.

    Tswv yim: Ua haujlwm ntawm chav tsev kub (15-25 ° C) nyob rau hauv tag nrho cov txheej txheem, tsis txhob ua dej khov da dej thiab tsis kub centrifugation.

           2.RNA tau raug degraded vim yog kev khaws cia tsis raug ntawm cov qauv lossis kev khaws cia ntev ntawm tus qauv.

    Cov lus pom zoo: Cov qauv sau tshiab yuav tsum tau khov sai sai hauv cov kua nitrogen, thiab tom qab ntawd khaws cia ntawm -80 ° C rau lub sijhawm ntev, tsis txhob rov khov thiab thawing ntawm cov qauv;lossis tam sim ntawd tsau cov qauv hauv RNA stabilizer RNAlater tov (cov qauv tsiaj txhu).

           3.Tsis txaus cov qauv fragmentation thiab lysis ua rau blockage ntawm purification kem.

    Tswv yim: Thaum sib tsoo cov ntaub so ntswg, thov xyuas kom meej tias cov ntaub so ntswg yog av txaus, thiab sai sai hloov mus rau lub pre-npaj Buffer PSL1 (kom paub meej tias qhov tseeb feem ntawm β-ME tau ntxiv, saib cov kauj ruam 1 ntawm cov txheej txheem).

    4.Qhov eluent tau ntxiv tsis raug.

    Tswv yim: Nco ntsoov tias RNase-Free ddH2O yog dripped rau hauv nruab nrab ntawm lub purification kem membrane.

    5.Qhov tseeb ntim ntawm ethanol tsis tau ntxiv rau Buffer PSL2 lossis Buffer PRW2.

    Tswv yim: Thov ua raws li cov lus qhia, ntxiv qhov tseeb ntim ntawm ethanol rau Buffer PSL2 thiab Buffer PRW2 thiab sib tov zoo ua ntej cov khoom siv.

    6.Cov qauv ntawm cov ntaub so ntswg tsis tsim nyog.

    Tswv yim: Siv 50 mg ntawm cov ntaub so ntswg rau 500 μl ntawm Buffer PSL1.Kev siv cov ntaub so ntswg ntau dhau yuav txo tau cov RNA rho tawm thiab qhov purity ntawm qhov tshwm sim RNA kuj yuav raug txo.Peb xav kom pom zoo tias thawj zaug koob tshuaj yuav tsum tsis txhob ntau tshaj 50 mg rau RNA rho tawm haujlwm.

    7. Tsis tsim nyog elution ntim lossis tsis tiav elution.

    Tswv yim: Lub ntim eluent ntawm purification kem yog 50-200 μl;Yog tias cov nyhuv elution tsis txaus siab, nws raug nquahu kom ncua lub sijhawm ntawm chav tsev kub tom qab ntxiv preheated RNase-Free ddH2O, xws li 5-10 min.

    8.Cov purification kem muaj ethanol residue tom qab ntxuav nrog Buffer PRW2.

       Cov lus qhia: Yog tias lub raj khoob yog centrifuged rau 1 min thiab tseem muaj ethanol seem tom qab ntxuav hauv Buffer PRW2, koj tuaj yeem nce lub sij hawm ntawm lub raj khoob centrifugation mus rau 2 min, los yog tso cov purification kem ntawm chav tsev kub rau 5 min kom tshem tawm cov ethanol seem.

    9.Cov khoom siv tau siv tsis raug.

       Tswv yim: Rau cov qauv cog ntawm polyphenolic polysaccharides, siv cov khoom siv xws li Plant Total RNA Isolation Kit yuav tsis tuaj yeem tau txais cov qauv RNA zoo tagnrho.Peb pom zoo kom koj siv Plant Total RNA IsolationKit Plus, uas yog tsim tshwj xeeb rau cov qauv cog polyphenolic polysaccharide.Cov khoom siv tshwj xeeb tsim los rau rho tawm RNA los ntawm polyphenol thiab polysaccharide cog cov qauv.

    OD260 / OD280 tus nqi qis

    RNA elution nrog ddH2O thiab siv rau kev nyeem ntawv spectrophotometer ua rau qis OD260 / OD280 qhov tseem ceeb.Peb pom zoo kom siv 10 mM Tris-HCl, pH 7.5 (tsis yog RNase-Free ddH)2O rau elute RNA) kom tau txais qhov tseeb OD260/OD280 qhov tseem ceeb, saib "RNA Concentration thiab Purification Assays" ntawm nplooj 19.

    Lub purified RNA yog degraded

    Qhov zoo ntawm purified RNA muaj feem xyuam rau yam xws li cov qauv khaws cia, RNase contamination, thiab manipulation.

    Kev tshuaj xyuas ntawm cov laj thawj uas tshwm sim:

    1.Cov ntaub so ntswg tsis tau muab khaws cia rau hauv lub sijhawm tom qab sau.

        Kev pom zoo: Yog tias cov ntaub so ntswg tsis siv rau lub sijhawm tom qab sau, thov khaws cia rau hauv cov kua nitrogen ntawm qhov kub thiab txias tam sim ntawd lossis hloov mus rau -80 ° C rau lub sijhawm ntev cia tom qab ceev ceev hauv cov kua nitrogen, lossis tam sim ntawd tso cov qauv hauv RNA stabilizer RNAlater tov (cov qauv tsiaj txhu).Rau RNA rho tawm, sim siv cov ntaub so ntswg tshiab tshiab.

    2.Repeated khov thiab thawing ntawm cov ntaub so ntswg kuaj.

       Cov lus qhia: Thaum khaws cov ntaub so ntswg, nws yog qhov zoo tshaj plaws los txiav rau hauv me me rau kev khaws cia, thiab tshem tawm ib feem ntawm lawv thaum siv lawv kom tsis txhob degradation ntawm RNA los ntawm kev rov ua kom khov thiab thawing ntawm cov qauv.

    3.RNase tau qhia hauv chav ua haujlwm lossis tsis hnav cov hnab looj tes, qhov ncauj qhov ntswg, thiab lwm yam.

       Tswv yim: RNA extraction thwmsim yog ua tau zoo tshaj plaws nyob rau hauv cais RNA kev khiav hauj lwm, thiab lub chav kuaj lub rooj yuav tsum tau ntxuav ua ntej qhov kev sim, thiab pov tseg hnab looj tes thiab lub qhov ncauj qhov ntswg yuav tsum tau hnav thaum lub sij hawm sim kom tsis txhob RNA degradation los ntawm kev qhia RNase mus rau qhov loj tshaj plaws.

    4.Cov reagent muaj paug los ntawm RNase thaum siv.

       Tswv yim: Hloov nrog ib tug tshiab series ntawm cov nroj tsuag tag nrho RNA extraction kits rau lwm yam thwmsim.

    5.Cov centrifuge raj thiab pipette cov lus qhia siv rau RNA manipulation yog kis nrog RNase.

    Cov lus qhia: Xyuas kom tseeb tias cov raj centrifuge, cov lus qhia pipette, pipettes, thiab lwm yam siv hauv RNA extraction yog txhua yam RNase-Free.

    Phau ntawv qhia:

    Plant Total RNA Isolation Kit Instruction Manual

    Sau koj cov lus ntawm no thiab xa tuaj rau peb