QuickEasyᵀᴹ Real Time PCR Kit-Taqman
Kit Description:
◮Qhov tshwj xeeb PCR optimization system ua rau 2 × QuickEasyTMTiag tiag PCR Mix-Taqman tau tshaj.
◮Kub-pib Foregene HS Taq Polymerase muaj kev ua kom zoo dua qub, ua kom muaj zog ntau dua, thiab muaj qhov ua kom muaj zog ntau dua.
◮2 × QuickEasyTMTiag PCR Mix -Taqman siv lub cim tshwj xeeb optimized los ntawm Foregene los txhim kho qhov rhiab heev thiab qhov tshwj xeeb ntawm kev sojntsuam tshwj xeeb, uas tuaj yeem siv rau genotyping thiab luam cov lej hloov pauv thiab tau txais cov txiaj ntsig raug.
◮Cov khoom no los nrog ROX sab hauv siv dye, uas tuaj yeem siv los tshem tawm cov teeb liab keeb kwm yav dhau thiab teeb liab yuam kev ntawm qhov dej, uas yooj yim rau cov neeg siv khoom siv ntau hom PCR cov cuab yeej.
Cov lus piav qhia
2 × QuickEasyTMTiag tiag PCR Mix-Taqman muab los ntawm QuickEasyTMLub Sijhawm PCR Cov Khoom Siv-Taqman yog cov khoom siv tshiab tiam tshiab tsim los rau Real Time PCR amplification cov tshuaj tiv thaiv siv cov tshuaj fluorescent tshwj xeeb.Nws cov tub ntxhais tseem ceeb Foregene HS Taq DNA Polymerase yog raws li kev hloov kho Antibody, siv nrog Foregene ntxiv kev ua kom zoo dua PCR system, muaj ntau dua qhov tshwj xeeb thiab kev ua kom zoo dua qub.Piv nrog rau PCR sib xyaw zoo tib yam, nws muaj peev xwm ua kom muaj zog ntau dua thiab qis dua qhov tsis sib xws.Nws tuaj yeem siv rau hauv fluorescence quantitative PCR cov tshuaj tiv thaiv kom txo tau qhov tsis yog qhov tshwj xeeb amplification thiab txhim kho qhov tseeb ntawm PCR.Nws tuaj yeem txhim kho cov khoom tshwj xeeb thiab cov tshuaj tiv thaiv rhiab heev.Nyob rau tib lub sijhawm, ROX tau muab los ua cov tshuaj siv sab hauv.
Lub QuickEasyTMLub Sijhawm PCR Cov Khoom Siv-Taqman muaj qhov dav dav ntawm daim ntawv thov rau tus qauv qauv, thiab tuaj yeem tau txais tus qauv nkhaus zoo hauv ntau thaj tsam sib txawv, thiab tuaj yeem txheeb xyuas qhov tseeb thiab txheeb xyuas cov hom phiaj nrog kev rov ua tau zoo thiab kev ntseeg siab.
Specifications
200 Preps,500 Preps,1000 Preps,2000 Preps
Cov khoom siv
| QuickEasyTMReal Time PCR Kit-Taqman(20ua m lqhov system) | ||||
| Cov khoom siv | QP-01121 | QP-01122 | QP-01123 | QP-01124 |
| 200 Preps | 500 Preps | 1000 Preps | 2000 Preps | |
| 2 ×CeevYooj yimTM PCR tiag Sib tov-Taqman | 1 ml ib×2 | 1.7ml ib×3 | 1.7ml ib×6 | 1.7ml ib×12 |
| 20×ROX Reference Dye | 200μL | 0.5 ml yog ' | 1 ml ib | 1 ml ib×2 |
| DNase-Dawb ddH2O | 1.7ml ib | 1.7ml ib×2 | 10 ml ib | 20 ml ib |
| IFU | 1ib daim | 1ib daim | 1ib daim | 1ib daim |
Nta & qhov zoo
■ Qhov tshwj xeeb PCR optimization system ua rau 2 × QuickEasyTMTiag tiag PCR Mix-Taqman tau tshaj.
■ Kub-pib Foregene HS Taq Polymerase muaj kev ua kom muaj zog ntau dua, ua kom muaj zog ntau dua, thiab muaj qhov ua kom muaj zog ntau dua.
■ 2× QuickEasyTM Tiag PCR Mix -Taqman siv lub cim tshwj xeeb optimized los ntawm Foregene los txhim kho qhov rhiab heev thiab qhov tshwj xeeb ntawm kev sojntsuam tshwj xeeb, uas tuaj yeem siv rau genotyping thiab luam cov lej hloov pauv thiab tau txais cov txiaj ntsig raug.
■ Cov khoom no los nrog ROX sab hauv siv dye, uas tuaj yeem siv los tshem tawm cov teeb liab keeb kwm yav dhau thiab cov teeb liab yuam kev ntawm qhov dej, uas yooj yim rau cov neeg siv khoom siv ntau hom PCR cov cuab yeej.
Daim ntawv thov Kit
■ Fluorescence quantitative PCR rau template quantitative analysis;
■ Ckev nthuav dav PCR;
■ Yuav siv tau rau kev kuaj pom allele.
Cia thiab Txee lub neej
Cov khoom siv yuav tsum khaws cia ntawm -20 ° C hauv qhov tsaus ntuj;Yog tias siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ° C rau lub sijhawm luv luv (siv li ntawm 10 hnub).
Real Time PCR primer tsim qauv
Forward Primer thiab Reverse Primer
Rau Real Time PCR, primer tsim yog ib qho tseem ceeb heev.Primers muaj feem xyuam rau qhov tshwj xeeb thiab kev ua haujlwm ntawm PCR amplification, thiab tuaj yeem tsim nrog siv rau cov hauv qab no:
Primer ntev: 18-30bp.
GC cov ntsiab lus: 40-60%.
Tus nqi Tm: Primer tsim software, xws li Primer 5, tuaj yeem muab tus nqi Tm ntawm primer.Tm qhov tseem ceeb ntawm cov dej ntws thiab cov dej hauv qab yuav tsum nyob ze li qhov ua tau.Cov mis laij lej Tm kuj tseem siv tau: Tm = 4 °C (G + C) + 2 ° C (A + T).Thaum ua PCR, qhov kub thiab txias hauv qab tus nqi primer Tm ntawm 5 ° C feem ntau yog xaiv raws li qhov kub thiab txias (qhov sib piv ntawm qhov kub ntawm qhov annealing tuaj yeem nce qhov tshwj xeeb ntawm cov tshuaj tiv thaiv PCR).
Primers thiab PCR khoom:
Tsim primer PCR amplification khoom ntev yog nyiam dua 100-150bp.
Tsim primers nyob rau hauv lub thib ob structural cheeb tsam ntawm lub template yuav tsum tau zam kom ntau li ntau tau.
Zam kev tsim ntawm 2 lossis ntau qhov sib ntxiv ntawm lub hauv paus ntawm 3' kawg ntawm cov dej ntws thiab cov dej hauv qab.
Primer 3′ davhlau ya nyob twg puag tsis tuaj yeem nrog 3 sib law liag G lossis C.
Cov primers lawv tus kheej tsis tuaj yeem muaj cov qauv sib xyaw ua ke, txwv tsis pub tus qauv hairpin yuav raug tsim, cuam tshuam rau PCR amplification.
ATCG yuav tsum tau muab faib raws li qhov sib npaug li qhov ua tau hauv cov txheej txheem primer, thiab 3 'terminal puag yuav tsum raug zam raws li T.
Daim Ntawv Ntxiv 1: Cell Direct RT-qPCR Cov Khoom Siv Khoom Siv Ntxiv Pob
1.Cell Lysis Solution
|
| |||
| Cov khoom siv (24-zoo lysis system / zoo) | DRT-01011-A1 | DRT-01011-A2 | |
| 100 T | 500 T | ||
| Ib feemKuv | Buffer CL | 20 ml ib | 100 ml |
| Foregene Protease Plus II | 400l ua | 1ml x 2 | |
| Buffer ST | 1ml x 2 | 10 ml | |
| Ib feemII | DNA Eraser | 400l ua | 1ml x 2 |
2. RT Sib
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| |
| Cov khoom siv (20 μl cov tshuaj tiv thaiv kab mob) | DRT-01011-B1 |
| 200 T | |
| 5 × Direct RT Mix | 800l ua |
| RNase-Dawb ddH2O | 1.7ml x 2 |
|
| ||
| Cov khoom siv (20 μl cov tshuaj tiv thaiv kab mob) | DRT-01011-C1 | DRT-01011-C2 |
| 200 T | 1000 T | |
| 2 × Direct qPCR Mix-SYBR | 1ml x 2 | 1.7ml x 6 |
| 50 × ROX Reference Dye | 40l ua | 200 mL |
| RNase-Dawb ddH2O | 1.7ml ib | 10 ml |
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