Stool DNA Isolation Kit DNA Extraction Purification Kits los ntawm quav
Kit Description:
Ua kom huv sai sai thiab tau txais cov txiaj ntsig zoo ntawm DNA genomic los ntawm ntau cov qauv quav.
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Kev piav qhia
Cov khoom siv no muab txoj hauv kev sai thiab yooj yim rau kev rho tawm genomic DNA los ntawm ntau qhov chaw ntawm av.Muaj ntau tus inhibitors xws li humic acid thiab hlau ions hauv cov qauv hauv av.Txawm hais tias cov tshuaj no muaj nyob rau hauv me me nyob rau hauv lub purified DNA, lawv yuav muaj kev cuam tshuam rau downstream tshua, xws li PCR thiab txwv enzyme digestion.Yog li ntawd, tus yuam sij rau purifying av DNA yog yuav ua li cas kom tshem tawm inhibitors nyob rau hauv cov av.
Cov khoom siv no siv DNA-Tsuas Kem uas tuaj yeem khi rau DNA, lub npe tshiab Foregene Protease thiab lub cev tsis sib xws, uas tuaj yeem tshem tawm ntau yam inhibitors los ntawm cov av yam tsis muaj cov organic hnyav rho tawm lossis ethanol thiab isopropanol los nag.Kev rho tawm DNA los ntawm cov qauv hauv av tuaj yeem ua tiav hauv 90 feeb.
Specifications
25 Preps, 50 Preps, 100 Preps, 250 Preps
Cov khoom siv
| Buffer SL1 |
| Buffer SL2 |
| Buffer SL3 |
| Buffer PW |
| Buffer WB |
| Buffer IB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase paug: DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sijhawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
- Ceev ceev: Kev khiav hauj lwm yog yooj yim, thiab fecal genomic DNA extraction lag luam tuaj yeem ua tiav hauv 40 feeb.
- Yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C qis-kub centrifugation los yog ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: Tsis tas yuav muaj cov organic reagent extraction.
-High zoo: Cov genomic DNA rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Nws yog qhov tsim nyog rau kev ua kom huv ntawm genomic DNA los ntawm cov qauv hauv qab no: cov tib neeg tshiab lossis khov, nyuj, nqaij qaib, nas thiab lwm yam fecal kuaj.
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ° C);Yog tias nws xav tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease tov muaj ib tug tshwj xeeb formula, uas yog active thaum khaws cia nyob rau hauv chav tsev kub rau lub sij hawm ntev (3 lub hlis), nws cov dej num thiab stability yuav zoo dua thaum khaws cia ntawm 4 ° C, yog li nws pom zoo kom khaws cia ntawm 4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20 ° C.
-Dry Lysozyme hmoov yog khaws cia ntawm -20 ° C, cov tshuaj Lysozyme npaj tau muab faib ua feem me me thiab khaws cia ntawm -20 ° C.
