Dej DNA Isolation Kit DNA Extraction thiab Purification Kit rau Dej
Kit Description:
Ceev ceev purify high-zoo genomic DNA los ntawm cov kab mob nyob rau hauv ntau yam qauv dej.
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Specifications
50 Preps
-Lysozyme: enzymatically hydrolyze cell phab ntsa ntawm cov kab mob zoo.
-Buffer TE: siv los npaj 100mg / ml Lysozyme tov thiab muab lysozyme enzymatic hydrolysis ib puag ncig.
-Buffer SG1 & Buffer SG2: Muab cov qauv protease digestion ib puag ncig.
-Foregene Protease: Enzymatically hydrolyze cov qauv hauv ib puag ncig protease enzymatic tso tawm genomic DNA.
-Buffer SG3: Inactivate Foregene Protease thiab muab qhov chaw thauj khoom DNA.
-Buffer SG4: Ntxiv los muab DNA chaw thau khoom ib puag ncig.
-Buffer PW: Tshem tawm impurities xws li protein thiab RNA hauv DNA.
-Buffer WB: Tshem tawm cov ntsev seem hauv DNA.
-Buffer EB: elute DNA ntawm purification kem membrane.
-DNA nkaus xwb: tshwj xeeb tshaj yog adsorb DNA genomic hauv lysate.
Cov khoom siv
| Buffer SG1 |
| Buffer SG2 |
| Buffer SG3 |
| Buffer SG4 |
| Buffer PW |
| Buffer WB |
| Buffer IB |
| Buffer TE |
| Foregene Protease |
| Lysozyme |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase kev kis kab mob: DNA-Tsuas Kab uas muab los ntawm cov khoom siv ua rau nws muaj peev xwm tshem RNA ntawm genomic DNA yam tsis muaj RNase ntxiv thaum lub sijhawm sim, zam lub chaw sim los ntawm cov kab mob exogenous RNase.
- Ceev ceev: Kev ua haujlwm yog yooj yim, thiab cov dej genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 40 feeb.
- Yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C qis-kub centrifugation los yog ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: tsis xav tau cov organic reagent extraction.
-High zoo: Cov genomic DNA tawg tawg yog loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Nws yog qhov tsim nyog rau kev ua kom huv ntawm genomic DNA ntawm cov qauv hauv qab no: aquaculture pas dej, dej pas dej, kais dej, pas dej, dej dej, dej pas dej thiab lwm yam qauv.
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ° C), yog tias nws yuav tsum tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37°C da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease tov muaj ib tug tshwj xeeb mis, uas yog active thaum khaws cia nyob rau hauv chav tsev kub ntev (3 lub hlis);Thaum khaws cia ntawm 4 ℃, nws cov haujlwm thiab kev ruaj ntseg yuav zoo dua, yog li nws pom zoo kom khaws cia ntawm 4 ℃, nco ntsoov tsis txhob khaws cia ntawm -20 ℃.
-Cov hmoov qhuav Lysozyme khaws cia ntawm -20 ° C;Cov tshuaj Lysozyme npaj tau muab faib ua feem me me thiab khaws cia ntawm -20 ° C.
