FFPE DNA Isolation Kit DNA Extraction Purification Kit los ntawm FFPE Cov Qauv
Kit Description:
Ceev ceev purify high-zoo genomic DNA los ntawm paran-embedded ntaub so ntswg xws li paraffin seem thiab paraffin blocks.
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Kev piav qhia
Cov khoom siv no tau mob siab rau kom rho tawm thiab ntxuav cov DNA zoo ntawm cov ntaub so ntswg formaldehyde lossis paraffin-embedded ntaub so ntswg.
Cov genomic DNA ntawm cov qauv paraffin-embedded yuav degraded vim lub sij hawm cia los yog ib puag ncig, ua rau nws nyuaj rau rho tawm cov genomic DNA.Cov khoom siv no tau txais cov qauv tsim tshwj xeeb, uas tsim nyog rau kev rho tawm genomic DNA los ntawm cov qauv paraffin-embedded nrog rau kev ua tau zoo ntawm kev ua tiav.Tag nrho cov txheej txheem tsis tas yuav siv tshuaj lom phenol, chloroform lossis isopropanol nag lossis daus.
Cov khoom siv no siv cov kab sib tw uas tuaj yeem khi DNA tshwj xeeb, cov khoom lag luam tshiab Foregene Protease Plus, thiab cov txheej txheem tsis sib xws, uas tuaj yeem tshem tawm cov khoom zoo paraffin-embedded cov ntaub so ntswg genomic DNA hauv 2-5 teev.
Specifications
50 Preps, 100 Preps, 250 Preps
Cov khoom siv
| Buffer FL1 |
| Buffer FL2* |
| Buffer PW* |
| Buffer WB |
| Buffer IB |
| Foregene Protease Plus |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase kab mob: Cov DNA-Tsuas Kab uas muab los ntawm cov khoom siv ua rau nws muaj peev xwm tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, zam lub chaw kuaj los ntawm exogenous RNase.
- Ceev ceev: Foregene Protease Plus muaj kev ua haujlwm siab dua li cov proteases zoo sib xws, thiab nws zom cov ntaub so ntswg sai dua;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 2-5 teev.
-Kev yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau 4°C low-temperature centrifugation lossis ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: tsis muaj cov organic reagent extraction yuav tsum tau.
-High zoo: Cov genomic DNA tawg tawg yog loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Nws yog tsim rau extraction thiab purification ntawm genomic DNA los ntawm formaldehyde-taw kuaj thiab paraffin-embedded ntaub so ntswg kuaj.
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ° C);Yog tias nws xav tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease Plus cov tshuaj muaj cov mis tshwj xeeb uas ua haujlwm thaum khaws cia hauv chav sov ntev (3 lub hlis);Nws muaj zog dua thiab ruaj khov thaum khaws cia ntawm 4 ° C, yog li nws pom zoo kom khaws cia ntawm 4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20 ° C.
