Ntshav DNA Mini Kit
Kit Description:
Ceev ceev purify high-zoo genomic DNA los ntawm anticoagulated ntshav (<1ml).
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Cov lus piav qhia
Cov khoom siv no tau txais cov tshiab Foregene Protease Plus thiab tshwj xeeb BL1 thiab BL2 tsis kab ke, uas tuaj yeem zom cov tshuaj tiv thaiv cov ntshav hauv lub sijhawm luv luv, yog li zam kom tsis txhob muaj DNA degradation mus rau qhov siab tshaj plaws thiab tau txais qhov siab tshaj plaws ntawm genomic DNA.Kev ua cov ntshav sai sai thiab cov DNA yooj yim-Tsuas yog kem ua haujlwm yooj yim heev rau kev tshem tawm cov ntshav genome, ua rau cov genomic DNA tuaj yeem tau txais cov khoom zoo thiab siab dawb huv hauv 40 feeb.
DNA-tsuas yog silica gel membrane siv nyob rau hauv centrifuge kem ntawm cov khoom siv no yog ib yam tshiab ntawm cov khoom tshwj xeeb rau peb lub tuam txhab, uas tuaj yeem khi DNA tau zoo thiab tshwj xeeb, thiab tuaj yeem tshem tawm RNA, impurities, proteins, ions thiab lwm yam organic tebchaw hauv hlwb mus rau qhov siab tshaj plaws.Cov DNA uas tau txais yog loj, siab purity, ruaj khov thiab txhim khu kev qha zoo.Lub peev xwm nqa tau siab tshaj plaws ntawm kab DNA-Tsuas yog 80 μg DNA.Cov DNA tau tuaj yeem siv rau hauv kev sim tshuaj molecular biology xws li enzyme zom, PCR, Southern hybridization thiab tsev qiv ntawv tsim.
Cov khoom siv tuaj yeem ua tiav txog li 1ml ntawm cov ntshav ntawm ib lub sijhawm kom tau txais cov nyiaj txaus ntawm cov purity genomic DNA.
Specifications
| Ntshav DNA Mini Kit | |||
| Cov khoom siv | TIAB SA-05111 | TIAB SA-05112 | TIAB SA-05113 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 15 ml ib | 30 ml | 75ml ib |
| Tsis muaj BL2 * | 15 ml ib | 30 ml | 75ml ib |
| Buffer DC | 100 mL | 200 ml | 500 ml |
| Buffer PW * | 25 ml ib | 50 ml ib | 125 ml ib |
| Buffer WB 1 | 15 ml ib | 30 ml | 75ml ib |
| Buffer IB | 10 ml ib | 20 ml ib | 50 ml ib |
| Foregene Protease Plus | 1.25 ml yog ' | 2.5 ml ib | 6.5ml ib |
| DNA-Tsuas Kem | 50 | 100 | 250 |
| Phau ntawv | 1 | 1 | 1 |
*: Buffer BL2 thiab Buffer PW muaj cov kua ntsev ua kom khaus.Thov hnav hnab looj tes thiab siv cov kev tiv thaiv tseem ceeb thaum ua haujlwm.
Nta & qhov zoo
-Tsis muaj RNase kab mob: Cov DNA-Tsuas Kab uas muab los ntawm cov khoom siv ua rau nws muaj peev xwm tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, zam lub chaw kuaj los ntawm exogenous RNase.
- Ceev ceev: Foregene Protease Plus muaj kev ua haujlwm siab dua li cov proteases zoo sib xws, thiab zom cov ntaub so ntswg sai dua;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 40 feeb.
-Kev yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, 4 ° C centrifugation los yog ethanol nag lossis daus ntawm DNA tsis tas yuav tsum tau.
-Kev nyab xeeb: Tsis siv cov organic reagent extraction.
-High zoo: Cov genomic DNA tawg tawg yog loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Nws yog tsim rau extraction thiab purification ntawm genomic DNA los ntawm tshiab los yog khov anticoagulated tag nrho cov ntshav.
Kev khiav haujlwm
Cia thiab txee lub neej
Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis hauv qhov chaw qhuav ntawm chav sov (15-25 ° C);Yog tias nws xav tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
Cov tshuaj Foregene Protease Plus muaj cov mis tshwj xeeb, uas ua haujlwm thaum khaws cia hauv chav sov ntev (3 lub hlis);Nws yuav muaj zog dua thiab ruaj khov thaum khaws cia ntawm 4 ° C, yog li nws pom zoo kom khaws cia ntawm 4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20 ° C.
