Cov Khoom Siv Cell Ncauj RT qPCR

Cov Khoom Siv Cell Ncauj RT qPCR

Cov Khoom Siv Qhia:

Cov khoom siv no siv cov cim lysis tsis zoo uas tuaj yeem tso sai sai RNA los ntawm kev kuaj kab mob ntawm tes rau RT-qPCR kev cuam tshuam, yog li tshem tawm lub sijhawm ua haujlwm thiab kev siv zog ua haujlwm RNA kev ua kom huv. RNA template tuaj yeem tau txais hauv 7 feeb xwb. Lub 5 × Ncaj Qha RT Mix thiab 2 × Ncaj QPCR Kev Sib Tw-SYBR reagents muab los ntawm cov khoom siv tau sai thiab muaj peev xwm tau txais cov txiaj ntsig tiag tiag ntawm lub sijhawm PCR.

5 RT Direct RT Mix thiab 2 × Ncaj QPCR Mix-SYBR muaj cov tshuaj tiv thaiv zoo, thiab lysate ntawm cov qauv tuaj yeem siv los ua tus qauv rau RT-qPCR ncaj qha. Cov khoom siv no muaj qhov tshwj xeeb RNA siab-kev sib raug zoo Foregene thim rov qab, thiab Kub D-Taq DNA polymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis haum, PCR optimizer thiab stabilizer.


Khoom Nthuav dav

Cov khoom lag luam

Cov Lus Qhia Tshwj Xeeb

200×20μl Rxns, 1000×20μl Rxns

Cov khoom siv Cheebtsam

Nqe I

Tsis CL

Foregene Protease Ntxiv II

Tsis ST

Ntu II

DNA Lwv

5 × Ncaj qha RT Mix

2 × Qhia ncaj qha qPCR Sib-SYBR

50 × ROX Reye Dye

RNase-DdH2O Dawb

Cov Lus Qhia

Nta & qhov zoo

■  Yooj yim thiab ua tau zoo: nrog Cell Direct RT thev naus laus zis, RNA cov qauv tuaj yeem tau txais hauv 7 feeb xwb.

■ Cov qauv xav tau yog qhov tsawg, tsawg li 10 cells tuaj yeem kuaj tau.

Through Muaj cov ntsiab lus siab: nws tuaj yeem tshawb pom sai RNA hauv cov hlwb tsim hauv 384, 96, 24, 12, 6-zoo daim hlau.

E DNA Eraser tuaj yeem tshem tawm cov genomes sai sai, txo qis cuam tshuam rau kev soj ntsuam tom ntej.

RT Qhov zoo tshaj ntawm RT thiab qPCR lub cev ua rau ob-kauj ruam RT-PCR thim rov qab ntau dua thiab PCR ntau qhov tshwj xeeb, thiab ntau dua tiv taus RT-qPCR cov tshuaj tiv thaiv tsis haum.

Daim ntawv thov cov khoom siv

Cov tau ntawm daim ntawv thov: kuaj hlwb.

- RNA tso tawm los ntawm cov qauv lysis: tsuas yog siv rau RT-qPCR tus qauv ntawm cov khoom siv no.

- Cov khoom siv tuaj yeem siv rau cov laj thawj nram qab no: kev tshuaj xyuas kev tshawb fawb ntawm cov noob, kev soj ntsuam ntawm siRNA-mediated gene silencing effect, tshuaj tshuaj, thiab lwm yam

Kev kos duab

Cell Direct RT qPCR diagram

Cia thiab txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum muab ceev cia li 4 ℃; Ntu II yuav tsum khaws cia ntawm -20 ℃.

 Foregene Protease Plus II yuav tsum khaws cia ntawm 4, Tsis txhob khov thaum -20 ℃.

 Ntshaw 2×Ncaj QPCR Mix-SYBR yuav tsum muab cia rau ntawm-20nyob hauv qhov tsaus ntuj; yog tias nquag siv, nws tseem tuaj yeem khaws cia ntawm 4℃ rau cov sijhawm khaws cia ntev (siv li 10 hnub).


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa rau peb

    Khoom pawg