Lnc-RT Heroᵀᴹ I(Nrog gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)
Specifications
25×20μl xws, 50×20μl r xws
Lnc-RT HeroTM I (Nrog gDNase) yog qhov kev hloov pauv rov qab tshwj xeeb tsim rau lncRNA kom tshem tawm cov kab mob DNA sai sai.5 × gDNase Mix tuaj yeem tshem tawm cov genome ntxiv hauv RNA ntawm 42 ° C rau 2 feeb, kom tsis txhob cuam tshuam ntawm genome ntawm qPCR cov txiaj ntsig.
5 × L-RT HeroTM Mix muaj Foregene LncRNA Reverse Transcriptase tshwj xeeb uas tsim los ntawm Foregene, uas yog ib hom tshiab ntawm kev rov qab transcriptase tshwj xeeb rau lncRNA thiab lwm yam ntev RNA cov qauv, nrog rau RNA affinity muaj zog thiab ntau dua reversal kaw efficiency.Lub kaw lus optimized ua rau tus nqi rov qab tau sai dua thiab tuaj yeem sau cov qauv RNA tau yooj yim nrog cov ntsiab lus GC siab thiab cov qauv theem nrab.Thawj strand cDNA synthesis tuaj yeem ua tiav ntawm 42 ° C hauv 15 feeb.
Cov khoom siv
| 5× gDNase Mix |
| 5× L-RT HeroTM Mix |
| RNase-Dawb ddH2O |
| Cov lus qhia |
Nta & qhov zoo
■Muaj peev xwm tshem tawm gDNA, uas tuaj yeem tshem gDNA hauv cov qauv hauv 2 feeb.
■ Nws tuaj yeem thim rov qab ntawm lncRNA tau zoo.Thaum cov khoom siv rov qab rov qab tau raug rau qPCR, tus nqi Ct qis dua li cov txheej txheem thim rov qab.
■ Kev hloov pauv hloov pauv tau zoo, nws tsuas yog siv sijhawm 15 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
■ Cov qauv tsis yooj yim: cov qauv uas muaj GC cov ntsiab lus siab thiab cov qauv txheej txheem nyuaj kuj tuaj yeem thim rov qab nrog kev ua haujlwm siab.
■ High-rhiab heev reverse transcription system, pg-theem templates kuj tau txais cDNA zoo.
■ Cov kev hloov pauv rov qab muaj cov thermal stability siab, qhov zoo tshaj plaws tiv thaiv kub yog 42℃, thiab nws tseem muaj qhov zoo rov qab ua haujlwm ntawm 50℃.
Daim ntawv thov Kit
LncRNA txheeb ze txheeb xyuas ntau yam.
LncRNA absolute quantitative analysis.
Nws tuaj yeem tshawb xyuas cov kab mob RNA sai thiab raug raws li tus kab mob RNA.
Rov qab hloov pauv ntawm RNA templates nrog siab GC cov ntsiab lus lossis cov txheej txheem thib ob nyuaj.
Kev khiav haujlwm
Cia thiab Txee lub neej
Cov khoom siv yuav tsum khaws cia ntawm -20°C. Khaws cov khoom hauv lub tub yees qhov kub tsis tu ncua ntawm -20°C tam sim ntawd tom qab tau txais.Yog tias qhov chaw cia khoom tsim nyog, cov khoom yuav tsis txo qis kev ua tau zoo thaum lub sijhawm siv tau 1 xyoos.
Phau ntawv qhia txog teeb meem
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-tshwj xeeb amplification
1. Tsis tsim nyog primer tsim
Kev pom zoo: Tsim primers raws li primer tsim qauv.
2. Genome residues.
Cov lus qhia: Nco ntsoov tias thawj kauj ruam ntawm gDNA tshem tawm cov tshuaj tiv thaiv kub yog 42 ° C, thiab lub sijhawm tshuaj tiv thaiv kuj tseem tuaj yeem txuas ntxiv mus rau 5min.
Tsis muaj amplification teeb liab los ntawm RT-qPCR
1. RNA yog degraded.
Kev pom zoo: Cov khoom siv rau RNA rho tawm yuav tsum yog qhov tshiab li sai tau, thiab cov khoom siv RNA zoo thiab siab huv yuav tsum tau siv.
2. RNA muaj cov tshuaj inhibitors.
Cov lus pom zoo: Cov tshuaj thim rov qab feem ntau suav nrog SDS, guanidine ntsev, EDTA, thiab lwm yam. Nws raug nquahu kom ntxuav RNA precipitate nrog 70% ethanol kom tshem tawm cov inhibitors.
3. Primer tsim teeb meem.
Kev pom zoo: Raws li cov qauv tsim primer, rov tsim cov primers rau kev tshuaj xyuas.
Lub hom phiaj band tshwm nyob rau hauv lub blank control
1. Kev sib kis ntawm cov cuab yeej khiav hauj lwm lossis cov tshuaj reagents.
Kev pom zoo: Tag nrho cov reagents los yog cov khoom siv rau qhov kev sim yuav tsum yog autoclaved.Ceev faj thiab maj mam thaum tuav kom tiv thaiv DNA cov qauv los ntawm kev nqus mus rau hauv lub pipette los yog nchuav tawm ntawm lub raj centrifuge.
2. Kev kis kab mob tshwm sim thaum lub sij hawm npaj ntawm PCR cov tshuaj tiv thaiv kab mob.
Kev pom zoo: Ua tib zoo ceev faj thaum tuav, xws li: hnav hnab looj tes latex, siv cov lus qhia lim.Siv Lub Sijhawm PCR Mix hauv lub kaw lus tsis muaj pov thawj.
3. Cov primers yog degraded.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov nrog primers tshiab rau fluorescence thwmsim.
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