Tsiaj Tag Nrho RNA Isolation Cov Khoom Siv Tag Nrho RNA Extraction thiab Purification Cov Khoom Siv rau Tsiaj Tsiaj & Cell
Kit Description:
Ceev ceev thiab ua tau zoo extract high-purity thiab high-zoo tag nrho RNA los ntawm ntau yam tsiaj cov ntaub so ntswg.
Tsis tas yuav txhawj txog RNA degradation.Tag nrho cov kab ke yog RNase-Free
Kev tshem tawm DNA zoo siv DNA-Tshuaj Kem
Tshem tawm DNA yam tsis tau ntxiv DNase
Yooj yim-tag nrho cov haujlwm ua tiav ntawm chav tsev kub
Fast-kev ua haujlwm tuaj yeem ua tiav hauv 30 feeb
Kev nyab xeeb - tsis muaj cov organic reagent siv
Siab purity-OD260/280≈1.8-2.1
Cov khoom piav qhia
50 Preps, 200 Preps
Cov khoom siv no siv lubspin kem thiab formulatsim los ntawm peb lub tuam txhab, uas muaj peev xwm extract high-purity thiab high-zoo tag nrho RNA los ntawm ntau yam tsiaj cov ntaub so ntswg nrog high efficiency.It muab ib tug npaum DNA-Tshuaj Kem, uas tau yooj yim cais thiab adsorb genomic DNA los ntawm supernatant thiab cov ntaub so ntswg lysate, yooj yim thiab txuag sij hawm;RNA nkaus xwb Kem tuaj yeem khi RNA zoo thiab tuaj yeem ua tiav ib txhij nrog cov qauv tshwj xeeb Ntau cov qauv.
Tag nrho cov kab ke yog RNase-Free, kom cov RNA rho tawm tsis degraded;Buffer RW1, Buffer RW2 tsis siv lub tshuab ntxhua khaub ncaws, kom cov RNA tau txais tsis muaj protein ntau, DNA, ion, thiab organic compound pollution.
Cov khoom siv
| Tsiaj Tag Nrho RNA Isolation Kit | ||
| Cov khoom siv | TSI-03011 | TSI-03014 |
| 50 T | 200 T | |
| Tsis muaj RL1 * | 25ml ib | 100 ml |
| Tsis muaj RL2 | 15ml ib | 60ml ib |
| Buffer RW1* | 25ml ib | 100 ml |
| Buffer RW2 | 24ml ib | ib 96ml |
| RNase-Dawb ddH2O | 10 ml | 40 ml |
| RNA nkaus xwb | 50 | 200 |
| DNA-Tswj Kem | 50 | 200 |
| Phau ntawv qhia | 1 qob | 1 qob |
Cov ntaub ntawv khoom
| Hom ntawv | Khiav kab | Purification tivthaiv | Foregene kem, reagent |
| Flux | 1-24 qauv | Sijhawm rau kev npaj | ~ 30 feeb (24 kuaj) |
| Centrifuge | Lub rooj centrifuge | Pyrolysis sib cais | Kev sib cais centrifugal |
| Qauv | Tsiaj cov ntaub so ntswg;cell | Cov qauv nqi | Cov ntaub so ntswg: 10-20 mg;Cell: (1-5) × 106 |
| Elution ntim | 50-200 hli | Max loading ntim | 850l ua |
Nta & qhov zoo
■ Tsis tas yuav txhawj txog RNA degradation;tag nrho cov kab ke yog RNase-Free
■ Tshem tawm DNA-siv DNA-Tshuaj Kem
■ Tshem tawm DNA yam tsis tau ntxiv DNase
■ Kev ua haujlwm yooj yim-txhua yam ua tiav ntawm chav tsev kub
■ Ceev -kev ua haujlwm tuaj yeem ua tiav hauv 30 feeb
■ Kev nyab xeeb-tsis muaj cov organic reagent yuav tsum tau
■ High purity -OD260/280≈1.8-2.1
Daim ntawv thov Kit
Nws yog qhov tsim nyog rau kev rho tawm thiab ua kom huv ntawm tag nrho RNA los ntawm ntau yam tshiab lossis khov tsiaj cov ntaub so ntswg lossis kab lis kev cai.
Khoom parameters
■ Cov ntawv thov hauv qab: thawj-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, thiab lwm yam.
■ Cov qauv: cov ntaub so ntswg tsiaj, cov kab lis kev cai
■ Siv: Cov ntaub so ntswg 10-20mg, Cells (2-5) × 106
■ Qhov siab tshaj plaws DNA khi muaj peev xwm ntawm purification kem: 80 μg
■ Elution ntim: 50-200 μl
