Tsiaj Tag RNA Rho Tawm Cov Khoom

Tsiaj Tag RNA Rho Tawm Cov Khoom

Cov Khoom Qhia:

Cov Khoom Siv:

Tsis RL1, Tsis RL2 

Tsis RW1, Tsis RW2

RNase-Dawb ddH2O, DNA-Tu Kab

RNA-Tsuas Kab

Kev qhia tshwj xeeb:

50 Preps, 200 Preps


Khoom nthuav dav

Khoom cim npe

Cov Khoom Qhia

Cov khoom siv no siv cov kab rov ua dua thiab cov qauv tsim los ntawm peb lub tuam txhab, uas tuaj yeem tshem tawm qhov ua kom huv thiab zoo tag nrho RNA los ntawm ntau cov nqaij nqaij nrog kev ua haujlwm siab. los ntawm supernatant thiab cov ntaub so ntswg lysate, yooj yim thiab txuag sijhawm; RNA-tsuas yog Kab tuaj yeem ua haujlwm zoo RNA thiab tuaj yeem ua tiav ib txhij nrog cov qauv tshwj xeeb Ntau qhov piv txwv.

Tag nrho cov txheej txheem yog RNase-Free, yog li ntawd RNA muab rho tawm tsis tau puas; Tsis RW1, Tsis RW2 tsis siv lub tshuab ntxhua khaub ncaws, kom cov RNA tau txais yog tsis muaj cov protein, DNA, ion, thiab cov organic ua pa phem.

Nta & qhov zoo

■ Tsis tas yuav txhawj xeeb txog RNA kev puas tsuaj; tag nrho cov kab ke yog RNase-Free
■ Tshem tawm DNA-siv DNA-Tu Kab
■ Tshem DNA yam tsis ntxiv DNase
■ Yooj yim-txhua txoj haujlwm tau ua tiav ntawm chav sov
■ Kev ua haujlwm nrawm tuaj yeem ua tiav hauv 30 feeb
■ Muaj kev nyab xeeb-tsis xav tau cov tshuaj reagent organic
Pur Siab dawb huv -OD260/280≈1.8-2.1

123

Daim ntawv thov khoom siv

Nws yog qhov tsim nyog rau kev rho tawm thiab ua kom huv ntawm tag nrho RNA los ntawm ntau yam tshiab ntawm cov nqaij mos los yog khov tsiaj los yog cov kab lis kev cai.

Khoom tsis

Applications Daim ntawv thov nqes hav: thawj-kab cDNA synthesis, RT-PCR, cov qauv molecular, Sab qaum teb Blot, thiab lwm yam.
■ Cov piv txwv: cov nqaij nqaij, cov kab lis kev cai
Os Dosage: Tissues 10-20mg, Cells (2-5) × 106
■ Cov peev txheej DNA siab tshaj plaws ntawm kev ua kom huv: 80 μg
Kev tshem tawm ntim: 50-200 μl

Kev khiav haujlwm

animal total RNA-simple workflow

Daim duab

3 Animal Total RNA Isolation Kit7

Tsiaj Tag Nrho RNA Rho Tawm Cov Khoom Kho 20mg
Kev kuaj nas tshiab, noj 5% tag nrho RNA 1% agar

Glycogel electrophoresis yog qhov ua tau
1: Spleen 2: Lub raum
3: Siab 4: Lub plawv

Cia thiab txee lub neej

Cov khoom tuaj yeem khaws cia rau 24 lub hlis ntawm chav sov (15-25 ℃) lossis 2–8 ℃ kom ntev dua. Tsis RL1 tuaj yeem khaws cia ntawm 4 ℃ rau 1 lub hlis tom qab ntxiv β- mercaptoethanol (xaiv tau).


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa tuaj rau peb

    Khoom pawg