RT Easy II (nrog gDNase) Master Premix rau thawj-strand cDNA synthesis rau Real Time PCR nrog gDNase
Kit Description:
-Kev muaj peev xwm tshem tawm gDNA, uas tuaj yeem tshem gDNA hauv cov qauv hauv 2 feeb.
-Txoj kev rov qab ua haujlwm tau zoo, nws tsuas yog siv sijhawm 15 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
-Cov qauv tsis yooj yim: cov qauv nrog cov ntsiab lus GC siab thiab cov qauv theem nrab kuj tuaj yeem thim rov qab nrog kev ua haujlwm siab.
-High-rhiab heev rov qab transcription system, pg-theem templates kuj tau txais high-zoo cDNA.
-Qhov kev hloov pauv rov qab muaj cov thermal stability siab, qhov ntsuas kub zoo tshaj plaws yog 42℃, thiab nws tseem muaj qhov zoo rov qab ua haujlwm ntawm 50℃.
Specifications
25 × 20µl rxns, 100 × 20µl rxns
RT Ib qho yooj yimTMII (Nrog gDNase) yog qhov kev hloov pauv rov qab tshwj xeeb uas tsim los rau Real Time PCR kom tshem tawm cov kab mob DNA sai sai.5 × gDNase Mix tuaj yeem tshem tawm cov genome ntxiv hauv RNA ntawm 42 ° C rau 2 feeb, kom tsis txhob cuam tshuam ntawm genome ntawm qPCR cov txiaj ntsig.
5 × RO-EasyTMMix muaj Foregene Reverse Transcriptase tshwj xeeb uas tsim los ntawm Foregene, uas yog ib hom tshiab ntawm kev rov qab transcriptase nrog muaj zog RNA affinity.Lub kaw lus optimized ua rau qhov rov qab hloov pauv sai dua, thiab thawj strand cDNA synthesis tuaj yeem ua tiav ntawm 42 ° C hauv 15 feeb.
Cov kev hloov pauv rov qab ntawm cov khoom siv no kuj tseem tuaj yeem ua haujlwm zoo ntawm 50 ° C.Yog li ntawd, nws yog qhov zoo dua rau cov qauv RNA nyuaj thiab tuaj yeem sau cov qauv RNA tau yooj yim nrog cov ntsiab lus GC siab thiab cov qauv theem nrab.
Cov khoom siv
| 5 × gDNase Mix |
| 5 × RO-EasyTMSib tov |
| RNase-Dawb ddH2O |
| Cov lus qhia |
Nta & qhov zoo
-Kev muaj peev xwm tshem tawm gDNA, uas tuaj yeem tshem gDNA hauv cov qauv hauv 2 feeb.
-Txoj kev rov qab ua haujlwm tau zoo, nws tsuas yog siv sijhawm 15 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
-Cov qauv tsis yooj yim: cov qauv nrog cov ntsiab lus GC siab thiab cov qauv theem nrab kuj tuaj yeem thim rov qab nrog kev ua haujlwm siab.
-High-rhiab heev rov qab transcription system, pg-theem templates kuj tau txais high-zoo cDNA.
-Qhov kev hloov pauv rov qab muaj cov thermal stability siab, qhov ntsuas kub zoo tshaj plaws yog 42℃, thiab nws tseem muaj qhov zoo rov qab ua haujlwm ntawm 50℃.
Daim ntawv thov Kit
Siv ncaj qha rau hauv Lub Sijhawm PCR txhawm rau txheeb xyuas cov noob qhia ntau.
Nws tuaj yeem tshawb xyuas cov kab mob RNA sai thiab raug raws li tus kab mob RNA.
Rov qab hloov pauv ntawm RNA templates nrog siab GC cov ntsiab lus lossis cov txheej txheem thib ob nyuaj.
Kev ua haujlwm
Cia thiab Txee lub neej
Cov khoom siv yuav tsum khaws cia ntawm -20°C.Muab cov khoom tso rau hauv lub tub yees qhov kub tsis tu ncua ntawm -20 ° C tam sim tom qab tau txais.Yog tias qhov chaw cia khoom tsim nyog, cov khoom yuav tsis txo qis kev ua tau zoo thaum lub sijhawm siv tau 1 xyoos.
