RT Easyᵀᴹ II Master Premix rau thawj-strand cDNA synthesis rau Real Time PCR
Kit Description:
- Kev ua haujlwm rov qab ua haujlwm tau zoo, nws tsuas yog siv sijhawm 20 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
- High-rhiab heev rov qab transcription system, pg-theem templates kuj tau txais cDNA zoo.
- Cov kev hloov pauv rov qab muaj cov thermal stability siab, cov tshuaj tiv thaiv kub ntawm qhov system tuaj yeem siab li 50 ℃, thiab nws muaj qhov zoo rov qab ua haujlwm.
- Txawm tias cov qauv RNA tsis huv (cawv txog li 60%, guanidine ntsev txog 750mM) tuaj yeem raug rov qab cov tshuaj tiv thaiv.
- 2 × RT LOS YOG-EasyTM Mix tau ntxiv qhov kev hloov pauv hloov pauv hloov pauv tau zoo (Random Primer, Oligo(dT)18 Primer).
Cov lus piav qhia
Foregene's 2 × RT LOS YOG-EasyTMKev sib xyaw ua ke tuaj yeem tsim cov thawj-strand cDNA ntawm qhov kub txog li 50 ° C, uas yog qhov tsim nyog rau qhov rov qab hloov cov tshuaj tiv thaiv ntawm cov txheej txheem theem nrab RNA cov qauv.
RNA purified nyob rau hauv lub chaw soj nstuam feem ntau muaj cawv thiab guanidine ntsev residues, uas muaj zog inhibitory zog ntawm feem ntau reverse transcriptases, uas ua rau tsis zoo reverse transcription los yog tsis tshua muaj reverse transcription efficiency.Foregene 2 × RT Mix qhia tau hais tias ua siab ntev rau cawv thiab guanidine ntsev.Qhov siab tshaj plaws kam rau dej cawv hauv cov qauv RNA yog 60%, thiab siab siab siab siab rau guanidine ntsev yog 750mM.Txawm tias tsis huv RNA tuaj yeem siv rau kev rov qab cov tshuaj tiv thaiv nrog Foregene 2 × RT OR-EasyTMSib tov.
2 × RT LOS YOG-EasyTMMix yog ib qho kev hloov pauv sai sai uas tsim tshwj xeeb rau Real Time PCR.Cov txheej txheem no muaj kev rov qab rov qab ua haujlwm tau zoo thiab tsis tas yuav ntxiv cov primers, thiab tuaj yeem ua tau zoo rov qab cov lus pom zoo ntawm tus nqi me me ntawm RNA templates.Cov tshuaj tiv thaiv tshwj xeeb ua rau cov tshuaj tiv thaiv RT yooj yim dua, sai dua thiab ua haujlwm tau zoo dua.Thawj strand cDNA synthesis tuaj yeem ua tiav hauv 20 feeb.Cov khoom no tuaj yeem siv ua ke nrog Foregene's fluorescent quantitative khoom Real Time PCR EasyTMkom tau txais cov txiaj ntsig kev sim siab zoo.
Specifications
| RT Ib qho yooj yimTMII Master Premix rau thawj-strand cDNA synthesis rau Real Time PCR | |||
| Cov khoom siv | Qauv: RT-01021 | Qauv: RT-01022 | Qauv: RT-01023 |
| 50T (10μl system) | 200T (10μl system) | 800T (10μl system) | |
| 2 × RT LOS YOG-EasyTMSib tov | 0.25 ml yog ' | 1ml ib | 1ml x 4 |
| RNase-Dawb ddH2O | 1.7ml ib | 1.7ml ib | 1.7ml x 3 |
| Phau ntawv qhia | 1 qob | 1 qob | 1 qob |
Nta & qhov zoo
- Kev ua haujlwm rov qab ua haujlwm tau zoo, nws tsuas yog siv sijhawm 20 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
- High-rhiab heev rov qab transcription system, pg-theem templates kuj tau txais cDNA zoo.
- Cov kev hloov pauv rov qab muaj cov thermal stability siab, cov tshuaj tiv thaiv kub ntawm qhov system tuaj yeem siab li 50 ℃, thiab nws muaj qhov zoo rov qab ua haujlwm.
- Txawm tias cov qauv RNA tsis huv (cawv txog li 60%, guanidine ntsev txog 750mM) tuaj yeem raug rov qab cov tshuaj tiv thaiv.
- 2 × RT LOS YOG-EasyTMMix tau ntxiv qhov kev hloov pauv hloov pauv hloov pauv tau zoo (Random Primer, Oligo(dT)18Primer).
Daim ntawv thov Kit
- Siv ncaj qha hauv Lub Sijhawm PCR txhawm rau txhawm rau txheeb xyuas cov noob qhia
- Nws tuaj yeem txheeb xyuas qhov me me ntawm RNA xws li RNA cov kab mob
- Rov qab hloov pauv ntawm RNA cov qauv nrog cov ntsiab lus GC siab lossis cov qauv theem nrab nyuaj
Kev khiav haujlwm
Cia thiab txee lub neej
1. Kev thauj mus los
Tag nrho cov txheej txheem ntawm kev thauj mus los ntawm qhov kub thiab txias, kom ntseeg tau tias cov khoom siv nyob rau hauv lub xeev ntawm <4 ° C.
2. Cia tej yam kev mob
RT Ib qho yooj yimTMII khaws cia ntawm -20 ° C.Muab cov khoom tso rau hauv lub tub yees qhov kub tsis tu ncua ntawm -20 ° C tam sim tom qab tau txais.Yog tias qhov chaw cia khoom tsim nyog, cov khoom yuav tsis txo qis kev ua tau zoo thaum lub sijhawm siv tau 1 xyoos.
