RT Easyᵀᴹ Kuv Master Premix rau thawj-strand cDNA synthesis
Kit Description:
- Kev ua haujlwm rov qab ua haujlwm tau zoo, nws tsuas yog siv sijhawm 25 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
- High-rhiab heev rov qab transcription system, pg-theem templates kuj tau txais cDNA zoo.
- Cov kev hloov pauv rov qab muaj cov thermal stability siab, cov tshuaj tiv thaiv kub ntawm qhov system tuaj yeem siab li 50 ℃,thiab nws muaj qhov zoo rov qab transcription kev ua tau zoo.
- Txawm tias cov qauv RNA tsis huv (cawv txog li 45%, guanidine ntsev txog 750mM) tuaj yeem raug cov tshuaj tiv thaiv rov qab.
Cov lus piav qhia
FOREGENE's 2 × RT Yooj YimTMKev sib xyaw ua ke tuaj yeem tsim cov thawj-strand cDNA ntawm qhov kub txog li 50 ° C, uas yog qhov tsim nyog rau qhov rov qab hloov cov tshuaj tiv thaiv ntawm cov txheej txheem theem nrab RNA cov qauv.
RNA purified nyob rau hauv lub chaw soj nstuam feem ntau muaj cawv thiab guanidine ntsev residues, uas muaj zog inhibitory zog ntawm feem ntau reverse transcriptases, uas ua rau tsis zoo reverse transcription los yog tsis tshua muaj reverse transcription efficiency.FOREGENE 2 × RT Yooj yimTMKev sib xyaw ua ke qhia tau hais tias muaj kev siab ntev heev rau cawv thiab guanidine ntsev, qhov siab tshaj plaws kam rau dej cawv hauv cov qauv RNA yog 45%, thiab siab siab siab siab rau guanidine ntsev yog 750mM.Txawm hais tias RNA tsis yog dawb huv, cov tshuaj tiv thaiv rov qab tuaj yeem ua tau nrog FORREGENE 2 × RT Mix.Cov tshuaj tiv thaiv tshwj xeeb ua rau cov tshuaj tiv thaiv RT yooj yim dua, sai dua thiab ua haujlwm tau zoo, thiab kev sib txuas ntawm thawj strand cDNA tuaj yeem ua tiav hauv 25 feeb.
Cov khoom no tuaj yeem siv ua ke nrog Foregene's fluorescent quantitative khoom Real Time PCR EasyTMkom tau txais cov txiaj ntsig kev sim siab zoo.
Specifications
| RT Ib qho yooj yimTMKuv Master Premix rau thawj-strand cDNA synthesis | ||
| Cov khoom siv | Qauv: RT-01011 | Qauv: RT-01012 |
| 25 zaug (20μl system) | 100 zaug (20μl system) | |
| 2 × RT EasyTM Mix | 0.25 ml yog ' | 1ml ib |
| Random Primer (50μM) | 50l ua | 200 mL |
| Oligo (dT) 18 Primer (50μM) | 50l ua | 200 mL |
| RNase-Dawb ddH2O | 1.7ml ib | 1.7ml ib |
| Phau ntawv qhia | 1 qob | 1 qob |
Nta & qhov zoo
- Kev ua haujlwm rov qab ua haujlwm tau zoo, nws tsuas yog siv sijhawm 25 feeb los ua kom tiav cov synthesis ntawm thawj strand cDNA.
- High-rhiab heev rov qab transcription system, pg-theem templates kuj tau txais cDNA zoo.
- Cov kev hloov pauv rov qab muaj cov thermal stability siab, cov tshuaj tiv thaiv kub ntawm qhov system tuaj yeem siab li 50 ℃, thiab nws muaj qhov zoo rov qab ua haujlwm.
- Txawm tias cov qauv RNA tsis huv (cawv txog li 45%, guanidine ntsev txog 750mM) tuaj yeem raug cov tshuaj tiv thaiv rov qab.
Daim ntawv thov Kit
- Cov lus qhia RT-PCR
- Synthesis ntawm cDNA rau cloning thiab qhia kev tshawb fawb
- Kev tshuaj xyuas ntawm cov txheej txheem primer txuas ntxiv ntawm qhov chaw pib transcription
- RACE (kev ua kom nrawm nrawm ntawm cDNA xaus)
- Linear RNA amplification
- Chip cim
Kev khiav haujlwm
Cia thiab txee lub neej
1.Kev thauj mus los
Tag nrho cov txheej txheem ntawm kev thauj mus los ntawm lub thawv txias txias, kom ntseeg tau tias cov khoom siv nyob rau hauv lub xeev ntawm <4 ℃.
2. Cia tej yam kev mob
RT Ib qho yooj yimTMKuv khaws cia ntawm -20 ° C.Muab cov khoom tso rau hauv lub tub yees qhov kub tsis tu ncua ntawm -20 ° C tam sim tom qab tau txais.Yog tias qhov chaw cia khoom tsim nyog, cov khoom yuav tsis txo qis kev ua tau zoo thaum lub sijhawm siv tau 1 xyoos.
