Foreasy Taq DNA Polymerase
Kit Description:
◮Siab tshwj xeeb: Cov enzyme muaj qee yam kev ua haujlwm kub pib.
◮Ceev Amplification: 10 sec / kb.
◮Cov qauv hloov tau yooj yim: tuaj yeem siv tau los ua kom muaj txiaj ntsig zoo GC Tus nqi siab, ntau yam nyuaj-rau-amplify DNA qauv.
◮Muaj zog fidelity: zoo tib yam Taq Enzyme 6 zaug.
◮Muaj zog thermal stability: Nws tuaj yeem muab tso rau ntawm 37 ° C rau ib lub lim tiam thiab tuav ntau tshaj 90% kev ua haujlwm.
Kev piav qhia
Foreasy Taq DNA Polymerase yog ib tug tshiab Taq enzyme qhia nyob rau hauv Escherichia coli engineering kab mob los ntawm noob recombination technology.Lub enzyme nws tus kheej muaj qee yam kev ua haujlwm kub pib thiab tuaj yeem siv rau cov pa PCR thiab qPCR;Nws muaj 5'→ 3' DNA polymerase kev ua haujlwm thiab 5'→ 3' exonuclease kev ua, tab sis tsis muaj 3' → 5' exonuclease kev ua.
Cov khoom siv
| Cheebtsam | IB-01011 | IB-01012 | IB-01013 |
| Foreasy Taq DNA Polymerase(5 U / L) | 5000 U (1 ml) | 50 KUB (10 mL) | 500 KUB (100 mL) |
| 2 × Taq Reaction Buffer | 25ml x 5 | 250ml x 5 | 500ml x 25 |
Nta & qhov zoo
- Siab tshwj xeeb: Cov enzyme muaj qee yam kev ua haujlwm kub pib.
- Ceev Amplification: 10 sec / kb.
- Cov qauv hloov tau yooj yim: tuaj yeem siv tau los ua kom muaj txiaj ntsig zoo GC Cov nqi siab, ntau yam nyuaj-rau-amplify DNA qauv.
- Muaj zog fidelity: zoo tib yam Taq Enzyme 6 zaug.
- Muaj zog thermal stability: Nws tuaj yeem muab tso rau ntawm 37 ° C rau ib lub lim tiam thiab tswj tau ntau dua 90% kev ua haujlwm
Daim ntawv thov Kit
Ntau yam PCR / qPCR systems thiab ncaj qha PCR systems
PCR amplification ntawm DNA fragments
DNA labeling
DNA sequencing
PCR A-tailed
U txhais
1U: Tus nqi ntawm cov enzyme xav tau los sib xyaw 10 nmol ntawm deoxynucleotides rau hauv cov kua qaub-insoluble teeb meem siv activated salmon phev DNA ua qauv / primer rau 30 feeb ntawm 74 ° C.
Kev mob tshwm sim
| Kub | Sijhawm | Lub voj voog |
| 37 ° C | 5 feeb | 1 |
| 94 ° C | 5 feeb | 1 |
| 94 ° C | 10 sec | 35 |
| 60 ° C | 10 sec | |
| 72 ° C | 20 sec/kb | |
| 72 ° C | 2 feeb | 1 |
Cia
-20 ± 5 ° C rau 2 xyoos lossis ntawm -80 ° C rau kev khaws cia ntev.
Tsis muaj amplification signals
1.Cov Taq DNA Polymerase nyob rau hauv cov khoom siv poob nws cov kev ua si vim yog qhov khoom siv tsis raug los yog tas sij hawm ntawm cov khoom siv.
Kev pom zoo: Paub meej tias qhov chaw cia khoom ntawm cov khoom siv;rov ntxiv qhov tsim nyog ntawm Taq DNA Polymerase rau PCR system lossis yuav ib lub Sijhawm PCR tshiab rau cov kev sim cuam tshuam.
2.Muaj ntau cov inhibitors ntawm Taq DNA Polymerase hauv DNA template.
Tswv yim: Rov kho tus qauv los yog txo tus nqi ntawm cov qauv siv.
3.Qhov Mg2+ concentration tsis haum.
Kev pom zoo: Mg2 + concentration ntawm 2 × Tiag PCR Mix peb muab yog 3.5mM.Txawm li cas los xij, rau qee qhov tshwj xeeb primers thiab cov qauv, Mg2 + concentration yuav siab dua.Yog li ntawd, koj tuaj yeem ncaj qha ntxiv MgCl2 kom ua kom zoo dua Mg2 + concentration.Nws raug pom zoo kom nce Mg2 + 0.5mM txhua lub sijhawm rau kev ua kom zoo.
4.Lub PCR amplification tej yam kev mob tsis haum, thiab cov primer sib lawv liag los yog concentration yog tsis tsim nyog.
Cov lus qhia: paub meej tias qhov tseeb ntawm cov txheej txheem primer thiab cov primer tsis tau degraded;Yog hais tias lub teeb liab amplification tsis zoo, sim txo qhov kub thiab txias thiab kho cov primer concentration kom tsim nyog.
5.Tus qauv ntawm tus qauv yog tsawg lossis ntau dhau.
Kev pom zoo: Ua cov qauv linearization gradient dilution, thiab xaiv cov template concentration nrog qhov zoo tshaj plaws PCR nyhuv rau Real Time PCR sim.
NTC muaj tus nqi fluorescence siab dhau lawm
1.Reagent paug tshwm sim thaum lub sijhawm ua haujlwm.
Kev pom zoo: Hloov nrog cov tshuaj reagents tshiab rau kev sim PCR tiag tiag.
2.Contamination tshwm sim thaum lub sij hawm npaj ntawm cov tshuaj tiv thaiv PCR.
Kev pom zoo: Siv cov kev tiv thaiv tsim nyog thaum lub sijhawm ua haujlwm, xws li: hnav cov hnab looj tes latex, siv lub taub hau nrog lub lim dej, thiab lwm yam.
3.Cov primers yog degraded, thiab qhov degradation ntawm cov primers yuav ua rau tsis yog tshwj xeeb amplification.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov lawv nrog primers tshiab rau Real Time PCR thwmsim.
Primer dimer lossis tsis tshwj xeeb amplification
1.Qhov Mg2+ concentration tsis haum.
Kev pom zoo: Mg2 + concentration ntawm 2 × Tiag PCR EasyTM Mix peb muab yog 3.5 mM.Txawm li cas los xij, rau qee qhov tshwj xeeb primers thiab cov qauv, Mg2 + concentration yuav siab dua.Yog li ntawd, koj tuaj yeem ncaj qha ntxiv MgCl2 kom ua kom zoo dua Mg2 + concentration.Nws raug pom zoo kom nce Mg2 + 0.5mM txhua lub sijhawm rau kev ua kom zoo.
2.Lub PCR annealing kub yog tsawg dhau.
Tswv yim: Ua kom lub PCR annealing kub los ntawm 1 ℃ lossis 2 ℃ txhua zaus.
3.Cov khoom PCR ntev dhau lawm.
Kev pom zoo: Qhov ntev ntawm cov khoom PCR tiag tiag yuav tsum nyob nruab nrab ntawm 100-150bp, tsis ntau tshaj 500bp.
4.Cov primers yog degraded, thiab degradation ntawm cov primers yuav ua rau cov tsos ntawm tej yam amplification.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov lawv nrog primers tshiab rau Real Time PCR thwmsim.
5.Qhov system PCR tsis tsim nyog, lossis lub kaw lus me dhau.
Tswv yim: PCR cov tshuaj tiv thaiv me me dhau lawm yuav ua rau qhov tseeb ntawm kev kuaj pom yuav txo qis.Nws yog qhov zoo tshaj plaws los siv cov tshuaj tiv thaiv uas tau pom zoo los ntawm cov cuab yeej PCR ntau kom rov ua dua qhov kev sim PCR tiag tiag.
Tsis zoo repeatability ntawm quantitative values
1.Cov cuab yeej ua haujlwm tsis zoo.
Cov lus qhia: Tej zaum yuav muaj qhov yuam kev ntawm txhua lub PCR qhov ntawm lub ntsuas, uas ua rau tsis zoo rov tsim dua thaum tswj qhov kub thiab txias.Thov xyuas raws li cov lus qhia ntawm cov cuab yeej sib txuas.
2.Cov qauv purity tsis zoo.
Kev pom zoo: Cov qauv tsis huv yuav ua rau qhov tsis zoo ntawm qhov kev sim, uas suav nrog cov purity ntawm cov qauv thiab cov primers.Nws yog qhov zoo tshaj plaws los ntxuav cov qauv, thiab cov primers yog qhov zoo tshaj plaws purified los ntawm SDS-PAGE.
3.Qhov kev npaj PCR thiab lub sijhawm khaws cia ntev dhau lawm.
Cov lus qhia: Siv lub sijhawm tiag tiag PCR system rau PCR sim tam sim tom qab kev npaj, thiab tsis txhob tso nws tseg ntev dhau.
4.Lub PCR amplification tej yam kev mob tsis haum, thiab cov primer sib lawv liag los yog concentration yog tsis tsim nyog.
Cov lus qhia: paub meej tias qhov tseeb ntawm cov txheej txheem primer thiab cov primer tsis tau degraded;Yog hais tias lub teeb liab amplification tsis zoo, sim txo qhov kub thiab txias thiab kho cov primer concentration kom tsim nyog.
5.Qhov system PCR tsis tsim nyog, lossis lub kaw lus me dhau.
Tswv yim: PCR cov tshuaj tiv thaiv me me dhau lawm yuav ua rau qhov tseeb ntawm kev kuaj pom yuav txo qis.Nws yog qhov zoo tshaj plaws los siv cov tshuaj tiv thaiv uas tau pom zoo los ntawm cov cuab yeej PCR ntau kom rov ua dua qhov kev sim PCR tiag tiag.
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