Tsiaj Daim ntaub ncaj qha PCR cov khoom siv

Tsiaj Daim ntaub ncaj qha PCR cov khoom siv

Cov Khoom Siv Qhia:

Cov khoom siv no siv cov cim lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm cov tsiaj cov nqaij mos rau PCR kev cuam tshuam, yog li nws tshwj xeeb tshaj yog rau kev ntsuas caj ces loj.

Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65°C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.

2× PCR Yooj YimTM Sib tov muaj qhov muaj peev xwm tiv taus zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj los kuaj raws li tus qauv tsim rau kev ua kom tau zoo thiab meej. Qhov kev lees paub no muaj cov Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis, PCR optimizer thiab stabilizer.

D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.


Khoom Nthuav dav

Cov khoom lag luam

Cov Lus Qhia Tshwj Xeeb

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Cov khoom siv Cheebtsam

Nqe I

Tsis AL

Foregene Protease

6 × DNA Chaw Tsim Khoom Tsis Muaj

Ntu II

2 × PCR Yooj YimTM Sib tov

Nta & qhov zoo

■  Tsis muaj sijhawm siv thiab kim DNA ua kom huv

■ Cov qauv xav tau yog me me, tsawg li 5mg tsiaj cov ntaub so ntswg tuaj yeem kuaj.

Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab kev sib tsoo yog qhov yuav tsum tau ua, thiab kev ua haujlwm yog yooj yim.

■ Qhov zoo ntawm PCR ua rau PCR muaj qhov ntau dua qhov siab dua thiab qhov muaj peev xwm siab dua rau PCR cov tshuaj tiv thaiv kev tiv thaiv.

Khoom siv tsis

Daim ntawv thov: ntau yam tsiaj ntawm cov leeg.

DNA tso tawm los ntawm cov qauv lysis: tsuas yog siv ua PCR qauv.

Cov khoom siv tuaj yeem siv rau cov laj thawj nram qab no: kev txheeb xyuas tus mob transgenosis, kev tua tsiaj ntawm tsiaj, thiab lwm yam.

Ua haujlwm ntws

wk flw-animal tissue direct PCR

Cia thiab txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum ceev cia thaum 2-8.

Lub reagent Buffer AL tuaj yeem khaws cia rau 12 lub hlis hauv kev mob qhuav; nws tuaj yeem khaws cia ntawm 2-8℃  rau ntev cia.

 Reagent Foregene Protease muaj cov qauv tsis sib xws. Txhawm rau kom nws cov haujlwm thiab ruaj khov, thov khaws ntawm 4tau 12 lub hlis.

 Reagent 6×DNA Chaw thau khoom tsis tuaj yeem khaws cia ntawm 4lossis -20tau ntev.

Ntu II ntawm cov khoom siv no yuav tsum ceev cia ntawm-20.

 Ntshaw 2×PCR Yooj YimTM Sib tov tuaj yeem khaws cia rau 12 lub hlis ntawm -20; yog tias nws nquag siv, nws kuj tseem tuaj yeem khaws cia ntawm 4rau cov sijhawm cia luv (siv sijhawm li 10 hnub).


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  • Sau koj cov lus ntawm no thiab xa rau peb

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