Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits los ntawm nas Tail
Kit Description:
Lub ntiaj teb cov khoom siv ceev tshaj plaws rau kev rho tawm nas tus Tsov tus tw genomic DNA, uas tuaj yeem rho tawm cov DNA zoo ntawm tus nas tail hauv 1 teev.
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Kev piav qhia
Vim yog cov qauv thiab cov khoom ntawm tus nas tail nws tus kheej, kev rho tawm ntawm nas tail genomic DNA ib txwm muaj teeb meem rau cov kws tshawb fawb: ib qho yog tias lub sijhawm ua haujlwm ntawm tus nas tail ntev thiab cov genomic DNA tau yooj yim degraded, lwm qhov yog qhov nyuaj rau kev lis kev cai thiab purity ntawm cov extracted genomic DNA yog tsawg.
Cov khoom siv no siv DNA-Tsuas Kab uas tuaj yeem khi DNA tshwj xeeb, lub npe tshiab Foregene Protease thiab cov txheej txheem tsis sib xws, uas tuaj yeem zom cov qauv nas tus Tsov tus tw hauv 45 feeb, yog li txo qis qhov degradation ntawm genomic DNA thiab txo cov sijhawm ua piv txwv.Cov khoom siv tuaj yeem rho tawm 10-20μg ntawm cov khoom zoo, siab dawb huv genomic DNA los ntawm 0.5-1cm menyuam yaus lossis neeg laus nas tails hauv 60-90min.
Cov khoom siv DNA nkaus xwb silica gel matrix siv nyob rau hauv kab rov tav yog cov khoom siv tshiab tshwj xeeb ntawm lub tuam txhab, uas tuaj yeem ua tau zoo thiab tshwj xeeb adsorb DNA.Qhov siab tshaj plaws adsorption muaj peev xwm rau DNA yog 80μg.Qhov tshwj xeeb tsis thiab elution system tuaj yeem ua kom tshem tawm RNA, Impurity proteins, ions thiab lwm yam organic tebchaw hauv lub cell.Cov genomic DNA tawg tau yog loj, siab purity, ruaj khov thiab txhim khu kev qha zoo, thiab qhov loj ntawm DNA tawg yog ruaj khov ntawm txog 23kb.
Cov khoom siv kuj tseem tuaj yeem siv los rho tawm lwm cov ntaub so ntswg tawm sab nraud nas tus Tsov tus tw, suav nrog nas pob ntseg, nas nqaij, nas daim siab thiab lwm yam ntaub so ntswg, kom ib qho khoom siv tuaj yeem siv rau ntau lub hom phiaj thiab ua tau raws li cov kev xav tau ntawm cov neeg siv rau kev sim sib txawv.
Specifications
50 Preps, 100 Preps, 250 Preps
Cov khoom siv
| Buffer TL1 |
| Tsis pub dhau TL2 * |
| Buffer PW* |
| Buffer WB |
| Buffer IB |
| Foregene Protease Plus |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase kab mob: Cov DNA-Tsuas Kab uas muab los ntawm cov khoom siv ua rau nws muaj peev xwm tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, zam lub chaw kuaj los ntawm exogenous RNase.
- Ceev ceev: Foregene Protease Plus muaj kev ua haujlwm siab dua li cov proteases zoo sib xws, thiab nws zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 40 feeb.
- Yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C qis-kub centrifugation los yog ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: Tsis tas yuav muaj cov organic reagent extraction.
-High zoo: Cov genomic DNA rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Nws yog qhov tsim nyog rau kev tshem tawm thiab ua kom huv ntawm genomic DNA los ntawm cov tshiab lossis khov loj thiab nas tails (tseem siv los ntxuav cov nas lossis nas nqaij thiab nas pob ntseg genomic DNA).
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis hauv qhov chaw qhuav ntawm chav sov (15-25 ° C);Yog tias nws xav tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease Plus cov tshuaj muaj cov mis tshwj xeeb, nws muaj zog thaum khaws cia hauv chav sov ntev (3 lub hlis);khaws cia ntawm 4 ° C, kev ua haujlwm thiab kev ruaj ntseg yuav zoo dua, yog li nws pom zoo kom khaws cia ntawm 4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20 ° C.
