Bacterial DNA Isolation Kit Cov kab mob Genomic DNA Extraction Purification Kits
Kit Description:
Ceev ceev purify high-zoo genomic DNA los ntawm cov kab mob nyob rau hauv lub logarithmic loj hlob theem.
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Kev piav qhia
Cov khoom siv no muab txoj hauv kev sai thiab yooj yim ntawm kev rho tawm genomic DNA los ntawm kab lis kev cai ntawm cov kab mob (Gram-negative thiab Gram-positive) los ntawm ntau qhov chaw;nws tuaj yeem ua cov kab mob tsawg dua 3ml ntawm cov kab mob hauv lub logarithmic loj hlob theem (1 × 109 kab mob).Cov khoom siv tau sib xyaw nrog kev ua haujlwm siab Foregene Protease, uas tso cai rau 15-50μg ntawm cov DNA zoo genomic kom muab rho tawm hauv 1 teev.Tsis tas li ntawd, cov khoom siv kuj tseem tuaj yeem tshem tawm cov khoom siv caj ces uas tsis yog cov genome, xws li plasmids, Cosmid, BAC, thiab lwm yam.
Cov khoom siv DNA nkaus xwb silica gel matrix siv nyob rau hauv kab rov tav yog cov khoom siv tshiab tshwj xeeb ntawm lub tuam txhab, uas tuaj yeem ua tau zoo thiab tshwj xeeb adsorb DNA.Qhov siab tshaj plaws adsorption muaj peev xwm rau DNA yog 80μg.Qhov tshwj xeeb tsis pub thiab elution system tuaj yeem ua kom tshem tawm RNA, Impurity proteins, ions thiab lwm yam organic tebchaw hauv hlwb.Cov extracted genomic DNA fragment yog loj, siab purity, ruaj khov thiab txhim khu kev qha zoo, thiab qhov loj ntawm DNA tawg yog ruaj khov ntawm txog 23kb.
Specifications
50 Preps, 100 Preps, 250 Preps
Cov khoom siv
| Buffer ML1 |
| Buffer ML2 |
| Buffer PW |
| Buffer WB |
| Buffer IB |
| Foregene Protease |
| Lysozyme |
| Buffer TE |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase kab mob: Tsis tas yuav ntxiv RNase kom tshem tawm RNA hauv DNA genomic, zam lub chaw kuaj mob los ntawm exogenous RNase.
- Ceev ceev: Foregene Protease muaj kev ua haujlwm siab dua li cov proteases zoo sib xws thiab tuaj yeem zom cov kab mob tawg hauv lub sijhawm luv luv.
-Txoj kev yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: tsis muaj cov organic reagent extraction yuav tsum tau.
-High zoo: Cov genomic DNA tawg tawg yog loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Cov khoom siv no yog tsim rau purifying genomic DNA ntawm cov qauv hauv qab no: Gram-negative bacteria thiab Gram-positive bacteria in the logarithmic growth phase.
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ° C);Yog tias nws xav tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease tov muaj ib tug tshwj xeeb mis, uas yog active thaum khaws cia nyob rau hauv chav tsev kub ntev (3 lub hlis);Thaum khaws cia ntawm 4 ° C, nws cov haujlwm thiab kev ruaj ntseg yuav zoo dua, yog li nws pom zoo kom khaws cia ntawm 4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20 ° C.
-Lysozyme yog cov hmoov qhuav, uas ua haujlwm thaum khaws cia ntawm 2-8 ° C rau lub sijhawm ntev (3 lub hlis).Txhawm rau khaws cia ntev dua, thov khaws cia ntawm -20 ° C.
