RT-qPCR Easyᵀᴹ (Ib kauj ruam)-Taqman
Kit Description:
- Ib-Cov khoom siv kauj ruam ua kom rov qab hloov pauv thiab PCR yuav tsum tau nqa tawm hauv tib lub raj, tsuas yog xav tau ntxiv cov qauv RNA, tshwj xeeb PCR primers thiab RNase-Free ddH2O.
- Kev txheeb xyuas ntau npaum li lub sijhawm ntawm tus kab mob RNA lossis kab RNA tuaj yeem ua tiav sai thiab raug.
- Cov khoom siv siv qhov tshwj xeeb Foregene thim rov qab reagent thiab Foregene HotStar Taq DNA Polymerase ua ke nrog cov tshuaj tiv thaiv tshwj xeeb txhawm rau txhim kho kev ua kom zoo dua qub thiab qhov tshwj xeeb ntawm cov tshuaj tiv thaiv.
- Cov tshuaj tiv thaiv zoo tshaj plaws ua rau cov tshuaj tiv thaiv muaj kev paub ntau dua, muaj zog thermal stability, thiab kam rau siab dua.
- RT-qPCR Ib qho yooj yimTM(Ib kauj ruam)-Taqman cov khoom siv los nrog ROX sab hauv siv dye, uas tuaj yeem siv los tshem tawm cov teeb liab keeb kwm yav dhau thiab cov teeb liab tsis raug ntawm qhov dej, uas yooj yim rau cov neeg siv kawg siv hauv cov qauv sib txawv ntawm cov khoom siv PCR ntau.
Cov lus piav qhia
Foregene One-Step RT-PCR Ib qho yooj yim series cov khoom lag luam paub txog kev sib koom ua ke los ntawm RNA rau ob-stranded DNA, uas yog, thim rov qab thiab PCR amplification tau ua tiav nyob rau hauv tib cov tshuaj tiv thaiv centrifuge raj thiab tib cov tshuaj tiv thaiv, yog li cov kauj ruam sim ua kom yooj yim, kev sim ua kom zoo, thiab kev sim ua kom zoo dua qub.
RT-qPCR Ib qho yooj yimTM (IbKauj ruam)-Taqmansiv Foregene HotStar Taq DNA Polymerase, qhov ua kom zoo dua Taqman qPCR system tuaj yeem ua tau sai thiab tshwj xeeb ua lub sijhawm tiag tiag RT-qPCR nrhiav kom pom cov kab RNA cov qauv.
Specifications
100 Rxns (20μl system)
500 Rxns (20μl system)
Cov khoom siv
| RT-qPCR EasyTM (Ib kauj ruam)-Taqman | ||
| Cov khoom siv | Qauv: RT-02131 | Qauv: RT-02132 |
| 100Rxns (20μl system) | 500 Rxns (20μl system) | |
| 2 × RT-qPCR Ib qho yooj yimTMMix-Taqman | 1ml ib | 1.7ml x 3 |
| 20 × ROX Siv Dye | 100 mL | 0.5 ml yog ' |
| RNase-Dawb ddH2O | 1.7ml ib | 1.7ml x 3 |
| Kev qhia siv | 1 | 1 |
Nta & qhov zoo
- Cov khoom siv ib kauj ruam ua rau rov qab sau thiab PCR yuav tsum tau ua nyob rau hauv tib lub raj, tsuas yog yuav tsum tau ntxiv cov qauv RNA, tshwj xeeb PCR primers thiab RNase-Free ddH2O.
- Kev txheeb xyuas ntau npaum li lub sijhawm ntawm tus kab mob RNA lossis kab RNA tuaj yeem ua tiav sai thiab raug.
- Cov khoom siv siv qhov tshwj xeeb Foregene thim rov qab reagent thiab Foregene HotStar Taq DNA Polymerase ua ke nrog cov tshuaj tiv thaiv tshwj xeeb txhawm rau txhim kho kev ua kom zoo dua qub thiab qhov tshwj xeeb ntawm cov tshuaj tiv thaiv.
- Cov tshuaj tiv thaiv zoo tshaj plaws ua rau cov tshuaj tiv thaiv muaj kev paub ntau dua, muaj zog thermal stability, thiab kam rau siab dua.
- RT-qPCR Ib qho yooj yimTM(Ib kauj ruam)-Taqman cov khoom siv los nrog ROX sab hauv siv dye, uas tuaj yeem siv los tshem tawm cov teeb liab keeb kwm yav dhau thiab cov teeb liab tsis raug ntawm qhov dej, uas yooj yim rau cov neeg siv kawg siv hauv cov qauv sib txawv ntawm cov khoom siv PCR ntau.
Cov khoom siv parameter
Daim ntawv thov Kit
- Cov khoom siv no yog siv rau kev tshawb nrhiav sai thiab raug ntawm cov kab mob RNA xws li RNA kab mob.
- Nws yog qhov tshwj xeeb tshaj yog tsim rau kev tshawb nrhiav sai ntawm RNA templates nrog cov ntsiab lus GC siab lossis cov qauv theem nrab.
Kev ua haujlwm
Cia tej yam kev mob
1.Kev thauj mus lostej yam kev mob
Tag nrho cov txheej txheem ntawm kev thauj mus los ntawm lub thawv txias txias, kom ntseeg tau tias cov khoom siv nyob rau hauv lub xeev ntawm <4 ℃.
2.Cia tej yam kev mob
Cov khoom siv yog khaws cia ntawm -20 ℃.Khaws cov khoom hauv lub tub yees qhov kub tsis tu ncua ntawm-20 ℃ tam sim tom qab tau txais.Yog tias qhov kev cia khoom tsim nyog, cov khoomyuav tsis degrade ib qho kev ua tau zoo thaum lub sijhawm siv tau 1 xyoos.
