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Cell-free DNA Isolation Kit

Kit Description:

 

Kev rho tawm ntawm cfDNA los ntawm cov qauv ntshav plasma
Catalog No. TQ01BT0050, TQ01BT0100

foregene zog


Product Detail

Khoom cim npe

Kev piav qhia

Cell-free DNA (cfDNA) yog ib feem ntawm cfDNA tso tawm ntawm cov hlwb mus rau hauv cov hlab ntsha uas tuaj yeem nqa cov ntaub ntawv lom neeg los ntawmqog, kab mob thiab fetuses.Kev soj ntsuam ntawm cfDNA muab cov cim tseem ceeb rau kev kuaj mob, kuaj pom thiab saib xyuas cov kab mob kev loj hlobsion.Tam sim no, cov thev naus laus zis no tau siv dav hauv kev tshawb nrhiav qog nqaij hlav, kev taw qhia tshuaj thiab kev kwv yees,Kev kuaj pom tus kab mob thiab tsis muaj kev cuam tshuam ua ntej yug menyuam.

Zoo nucleic acid yog qhov lav rau kev tshawb fawb genomics.Foregene siv superparamagnetic hlaws purification technologymuab cov neeg siv nrog cov tshuaj rhiab heev nucleic acid extraction uas yooj yim ua tiav cov kab nucleic acid extraction rau qisconcentration.Raws li kev txheeb xyuas los ntawm ntau txhiab tus qauv kuaj mob, cov khoom no tuaj yeem tshem tawm cfDNA zoo los ntawm lub cev tsis muaj cell.xws li cov plasma tshiab los yog khov, ntshav, kua pleural, ascites thiab cerebrospinal kua rau kev tshawb fawb thiab kev kuaj mob hauv vitro diagnosticsiv.

Lub Foregene Cell-free DNA Isolation Kit yog tsim los rau kev cais cov cell-dawb DNA (cfDNA) los ntawm tib neeg cov ntshav, ntshav, kua dej hauv lub cev, lossis kuaj zis.Cov khoom siv tau sai thiab yooj yim cais cfDNA hauv peb kauj ruam: lysis / khi, ntxuav thiab elution.Nws siv tshwj xeeb tsim silica-coated superparamagnetic hlaws dai nrog lub cim lysis/binding system kom rov qab circulating nucleic acids los ntawm hydrogen bonding thiab electrostatic rog, tsis muaj adsorbing proteins thiab lwm yam impurities.Nws yog tsim rau high- throughput automated extraction chaw ua hauj lwm nyob rau hauv kev npaj rau tej downstream tsom xam siv PCR los yog NGS.

Cov khoom siv cov ntsiab lus thiab Cia

Table 1 Foregene Cell-dawb DNA Isolation Kit

Ckev xav Rxav Tus nqi(25 T) Tus nqi(50 T) Tus nqi(100 T) Storage
  Box1 Buffer AL 42mlx 1 89mx1 qw 1 89ml x 10-30 ° C
Buffer AW 1 38mlx 1 83mx1 qw 165ml x 1 fwj 10-30 ° C
Buffer AW 2 15ml x 1 fwj 33mlx 1 66mlx 1 10-30 ° C
Elution 3mlx1 ib 6mlx1 qw 12ml x 1 fwj 10-30 ° C
 Lub thawv 2 Magnetic Beads A 3.2mlx 1 6.5mlx 1 13mlx 1 2 - 8 ° C
Proteinase K 5mlx1 ib 10.5 mL x 1 fwj 21mlx1 qw 2 - 8 ° C

Nco tseg:

[1] Tsis txhob sib xyaw cov khoom sib txawv ntawm cov khoom siv sib txawv.

[2] Buffer AL tuaj yeem tsim precipitates, uas tsis cuam tshuam rau nws txoj haujlwm.Yog tias pom nag lossis daus, thov tso lub raj mis reagent rau hauv 56 ° C dej da dej rau 10 - 20 min kom txog thaum cov dej nag yaj.Tom qab ntawd sib tov kom huv si ua ntej siv.

[3] Tsis txhob khov lub Magnetic Beads A.

Txheej txheem

Nta & qhov zoo

-Kev Extraction zoo      High DNA extraction tawm los, zoo purity, zoo tshem tawm impurities thiab downstream PCR inhibitors;

Flexible Sample IncorporationIb qho yooj yim rho tawm ntawm cfDNA los ntawm 0.5mL mus rau 4mL kuaj nrog elution ntim tsawg li 20uL

Kev siv ntau yamValidated los ntawm cov qauv kuaj, tsim los rau qog nqaij hlav, kis kab mob thiab ua ntej yug menyuam nrog kua biopsy

Kev txhawb nqa dav ntawm Automated Platform  Tau tshaj phau ntawv ans automated extraction txheej txheem, txhawb Tecan, Hamilton, PE thiab lwm yam nucleic acid extraction workstations.


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa tuaj rau peb