Cell-free DNA Isolation Kit
Kev piav qhia
Cell-free DNA (cfDNA) yog ib feem ntawm cfDNA tso tawm ntawm cov hlwb mus rau hauv cov hlab ntsha uas tuaj yeem nqa cov ntaub ntawv lom neeg los ntawmqog, kab mob thiab fetuses.Kev soj ntsuam ntawm cfDNA muab cov cim tseem ceeb rau kev kuaj mob, kuaj pom thiab saib xyuas cov kab mob kev loj hlobsion.Tam sim no, cov thev naus laus zis no tau siv dav hauv kev tshawb nrhiav qog nqaij hlav, kev taw qhia tshuaj thiab kev kwv yees,Kev kuaj pom tus kab mob thiab tsis muaj kev cuam tshuam ua ntej yug menyuam.
Lub Foregene Cell-free DNA Isolation Kit yog tsim los rau kev cais cov cell-dawb DNA (cfDNA) los ntawm tib neeg cov ntshav, ntshav, kua dej hauv lub cev, lossis kuaj zis.Cov khoom siv tau sai thiab yooj yim cais cfDNA hauv peb kauj ruam: lysis / khi, ntxuav thiab elution.Nws siv tshwj xeeb tsim silica-coated superparamagnetic hlaws dai nrog lub cim lysis/binding system kom rov qab circulating nucleic acids los ntawm hydrogen bonding thiab electrostatic rog, tsis muaj adsorbing proteins thiab lwm yam impurities.Nws yog tsim rau high- throughput automated extraction chaw ua hauj lwm nyob rau hauv kev npaj rau tej downstream tsom xam siv PCR los yog NGS.
Cov khoom siv cov ntsiab lus thiab Cia
Table 1 Foregene Cell-dawb DNA Isolation Kit
Ckev xav | Rxav | Tus nqi(25 T) | Tus nqi(50 T) | Tus nqi(100 T) | Storage |
Box1 | Buffer AL | 42mlx 1 | 89mx1 qw | 1 89ml x | 10-30 ° C |
Buffer AW 1 | 38mlx 1 | 83mx1 qw | 165ml x 1 fwj | 10-30 ° C | |
Buffer AW 2 | 15ml x 1 fwj | 33mlx 1 | 66mlx 1 | 10-30 ° C | |
Elution | 3mlx1 ib | 6mlx1 qw | 12ml x 1 fwj | 10-30 ° C | |
Lub thawv 2 | Magnetic Beads A | 3.2mlx 1 | 6.5mlx 1 | 13mlx 1 | 2 - 8 ° C |
Proteinase K | 5mlx1 ib | 10.5 mL x 1 fwj | 21mlx1 qw | 2 - 8 ° C |
Nco tseg:
[1] Tsis txhob sib xyaw cov khoom sib txawv ntawm cov khoom siv sib txawv.
[2] Buffer AL tuaj yeem tsim precipitates, uas tsis cuam tshuam rau nws txoj haujlwm.Yog tias pom nag lossis daus, thov tso lub raj mis reagent rau hauv 56 ° C dej da dej rau 10 - 20 min kom txog thaum cov dej nag yaj.Tom qab ntawd sib tov kom huv si ua ntej siv.
[3] Tsis txhob khov lub Magnetic Beads A.
Txheej txheem
Nta & qhov zoo
-Kev Extraction zoo High DNA extraction tawm los, zoo purity, zoo tshem tawm impurities thiab downstream PCR inhibitors;
Flexible Sample IncorporationIb qho yooj yim rho tawm ntawm cfDNA los ntawm 0.5mL mus rau 4mL kuaj nrog elution ntim tsawg li 20uL
Kev siv ntau yamValidated los ntawm cov qauv kuaj, tsim los rau qog nqaij hlav, kis kab mob thiab ua ntej yug menyuam nrog kua biopsy
Kev txhawb nqa dav ntawm Automated Platform Tau tshaj phau ntawv ans automated extraction txheej txheem, txhawb Tecan, Hamilton, PE thiab lwm yam nucleic acid extraction workstations.