Genomic DNA Micro Kit Micro Genomic DNA Mini Cov khoom siv
Kit Description:
Ua kom huv sai sai rau cov qauv me me kom tau txais txiaj ntsig zoo genomic DNA.
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Kev piav qhia
Cov khoom siv no siv DNA-Tsuas Kab uas tuaj yeem khi DNA, ib qho tshiab Foregene Protease thiab lub cev tsis zoo, uas tuaj yeem tshem tawm ntau yam kab mob lom neeg, xws li kab mob ntshav, hlwb, thiab tsiaj txhu hauv 20-80 feeb.Cov ntaub so ntswg, cov hlab ntsha qhuav, cov chaw kho mob paj rwb swabs, cov hauv paus plaub hau, thiab lwm yam, yog purified kom tau txais high-concentration, high-zoo genomic DNA.
Specifications
50 Preps
Cov khoom siv
| Buffer ST1 |
| Tsis muaj ST2 * |
| Buffer PW* |
| Buffer WB |
| Buffer IB |
| Foregene Protease |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase paug: DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sijhawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
- Ceev ceev: Foregene Protease muaj kev ua haujlwm siab dua li cov proteases zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
- Yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C qis-kub centrifugation los yog ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: Tsis tas yuav muaj cov organic reagent extraction.
-High zoo: Cov genomic DNA rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
-Micro-elution system: Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Daim ntawv thov Kit
Nws yog qhov tsim nyog rau kev ua kom huv ntawm cov qauv hauv qab no ntawm genomic DNA: cov ntaub so ntswg tshiab lossis khov tsiaj, tshiab lossis anticoagulated cryopreserved kab ntawm cov ntshav tag nrho, cov ntshav qhuav, cov hlwb, cov ntaub so ntswg microsection, cov hauv paus plaub hau, cov tshuaj yaug qhov ncauj, thiab lwm yam.
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ° C);Yog tias nws xav tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8 ° C.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease tov muaj ib tug tshwj xeeb mis, uas yog active thaum khaws cia nyob rau hauv chav tsev kub ntev (3 lub hlis);nws cov dej num thiab kev ruaj ntseg yuav zoo dua thaum khaws cia ntawm 4°C, yog li nws pom zoo kom khaws cia ntawm4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20 ° C.
