Ntshav DNA Midi Kit (1-5ml) Ntshav Isolation Midi Cov Khoom Siv rau DNA los ntawm Ntshav
Kit Description:
Ceev ceev purify high-zoo genomic DNA los ntawm anticoagulated ntshav (1-5ml).
◮Tsis muaj RNase contamination:DNA-Tsuas Kem muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
◮Ceev ceev:Foregene Protease muaj kev ua haujlwm siab dua li cov protease zoo sib xws, thiab zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 20-80 feeb.
◮Yooj yim:Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau rau 4 ° C low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA.
◮Kev nyab xeeb:Tsis muaj cov organic reagent extraction yog yuav tsum tau.
◮High quality:Cov genomic DNA uas tau muab rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
◮Micro-elution system:Nws tuaj yeem ua kom cov concentration ntawm genomic DNA, uas yooj yim rau kev tshawb nrhiav los yog kev sim.
Kev piav qhia
Cov ntshav kuaj muaj ntau cov qe ntshav liab thiab platelet hlwb, uas ua rau cov ntshav ua teeb meem ntau dua.Txawm li cas los xij, cov ntshav genomic DNA rho tawm los ntawm txoj kev dav dav tsis yog siab purity thiab cov ntsiab lus tsawg dhau.Cov khoom siv no siv cov khoom lag luam tshiab Foregene Protease Plus thiab tshwj xeeb BL1 thiab BL2 tsis siv lub tshuab, uas tuaj yeem zom tag nrho cov ntshav anticoagulated thiabcoagulationkuaj nyob rau hauv lub sij hawm luv tshaj, yog li zam DNA degradation mus rau qhov loj tshaj plaws thiab tau txais qhov loj tshaj plaws ntawm genomic DNA.Cov ntshav ua haujlwm ceev ceev thiab kev ua haujlwm yooj yim spin kem ua kom yooj yim rau kev tshem tawm cov ntshav genome, kom cov DNA zoo, siab dawb huv genomic tuaj yeem tau txais hauv 40 feeb.
DNA-tsuas yog silica gel membrane siv nyob rau hauv kab kem ntawm cov khoom siv no yog cov khoom siv tshiab tshwj xeeb ntawm lub tuam txhab, uas muaj txiaj ntsig zoo thiab tshwj xeeb adsorbs DNA, thiab tuaj yeem tshem tawm RNA, impurity proteins, ions thiab lwm yam organic tebchaw hauv hlwb mus rau qhov loj tshaj.Cov DNA uas tau txais yog loj, nrog rau siab purity, ruaj khov thiab txhim khu kev qha zoo, thiab lub siab tshaj plaws nqa peev xwm ntawm ib tug spin kem yog 80 μg DNA.Cov DNA tau txais tuaj yeem siv rau kev sim molecular biology xws li kev txwv kev zom zaub mov, PCR, Southern hybridization, kev tsim tsev qiv ntawv thiab lwm yam.Cov khoom siv tuaj yeem ua tiav txog li 5ml ntawm cov ntshav ntawm ib lub sijhawm kom tau txais cov nyiaj txaus ntawm cov purity genomic DNA.
Specifications
50 Preps, 100 Preps, 250 Preps
Cov khoom siv
| Buffer BL1 |
| Tsis muaj BL2 * |
| Buffer DC |
| Buffer PW* |
| Buffer WB 1 |
| Buffer IB |
| Foregene Protease Plus |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis muaj RNase kab mob: Cov DNA-Tsuas Kab uas muab los ntawm cov khoom siv ua rau nws muaj peev xwm tshem RNA los ntawm genomic DNA yam tsis tau ntxiv RNase thaum lub sij hawm sim, zam lub chaw kuaj los ntawm exogenous RNase.
- Ceev ceev: Foregene Protease Plus muaj kev ua haujlwm siab dua li cov proteases zoo sib xws, thiab nws zom cov ntaub so ntswg sai;kev ua haujlwm yog yooj yim, thiab cov genomic DNA rho tawm haujlwm tuaj yeem ua tiav hauv 40 feeb.
- Yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, thiab tsis tas yuav tsum tau 4°C low-temperature centrifugation lossis ethanol nag lossis daus ntawm DNA.
-Kev nyab xeeb: Tsis tas yuav muaj cov organic reagent extraction.
-High zoo: Cov genomic DNA rho tawm muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, uas tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Nws yog tsim rau extraction thiab purification ntawm genomic DNA los ntawm tshiab los yog khov anticoagulated tag nrho cov ntshav.
Kev ua haujlwm
Cia thiab Txee lub neej
-Cov khoom siv no tuaj yeem khaws cia rau 12 lub hlis hauv qhov chaw qhuav ntawm chav sov (15-25℃);yog tias nws yuav tsum tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia ntawm 2-8℃.
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Ua ntej siv, nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau ib lub sijhawm.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov ua ntej siv.
-Foregene Protease Plus cov tshuaj muaj ib tug tshwj xeeb mis, uas yog active thaum khaws cia nyob rau hauv chav tsev kub rau lub sij hawm ntev (3 lub hlis), nws cov dej num thiab kev ruaj ntseg yuav zoo dua thaum khaws cia ntawm 4 ° C, yog li nws pom zoo kom khaws cia ntawm 4 ° C, nco ntsoov tsis txhob khaws cia ntawm -20.℃.
