Tuam Tshoj Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Ntaus Tail) (rau Genotyping) Chaw tsim tshuaj paus thiab tsum |Foregene

Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Ntaus Tail) (rau Genotyping)

Kit Description:

Tus nas tail lysate yog siv ncaj qha los ua tus qauv rau PCR amplification yam tsis muaj cais tawm ntawm DNA, uas yog ceev thiab rhiab heev.Ntxiv UNG los tiv thaiv kev ua qias tuaj kom tshem tawm cov khoom cuav.

◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.

◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.

◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.

◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.

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Product Detail

Khoom cim npe

Cov lus piav qhia

Cov khoom siv no siv cov txheej txheem tshwj xeeb lysis tsis tuaj yeem tso tawm genomic DNA sai sai los ntawm nas tails, pob ntseg, cov leeg thiab lwm yam ntaub so ntswg kuaj rau PCR cov tshuaj tiv thaiv, yog li nws yog qhov tshwj xeeb tshaj yog rau kev kuaj kab mob loj.

Cov txheej txheem ntawm kev tso genomic DNA los ntawm lysis tsis yog ua tiav hauv 10-30 feeb ntawm 65 ° C.Tsis muaj lwm yam txheej txheem xws li protein thiab RNA tshem tawm, thiab cov kab DNA tso tawm tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.

2 × M1-PCR Ib qho yooj yim^TMMix (UNG) siv dUTP hloov dTTP raws li 2 × M1-PCR Yooj Yim^TMSib tov (UNG), thiab ntxiv UNG enzyme (Uracil-N-glycosylase) uas tuaj yeem degrade cov qauv uas muaj dUTP tib lub sijhawm.Ua ntej cov tshuaj tiv thaiv PCR, UNG enzyme yog siv los degrade cov khoom PCR uas muaj uracil.Lub UNG enzyme yuav tsis muaj kev cuam tshuam rau tus qauv uas tsis muaj uracil, yog li ua kom muaj qhov tshwj xeeb thiab qhov tseeb ntawm kev nthuav dav thiab tiv thaiv qhov muaj peev xwm ntawm kev kuaj caj ces loj.Kev kis kab mob ntawm cov khoom PCR tau tshwm sim.

D-Taq DNA polymerase yog DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau cov tshuaj PCR ncaj qha.D-Taq DNA polymerase muaj kev ua siab ntev rau ntau yam PCR cov tshuaj tiv thaiv, thiab tuaj yeem ua kom muaj txiaj ntsig zoo ntawm DNA hauv ntau cov tshuaj tiv thaiv nyuaj, thiab qhov kev nthuav dav tuaj yeem ncav cuag 2Kb / min, uas yog tshwj xeeb tshaj yog haum rau Direct PCR cov tshuaj tiv thaiv.

Specifications

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Cov khoom siv

Ntu I	Buffer MP
	Foregene Protease Plus
	6 × DNA Loading Buffer
Ntu II	2 × M1 PCR Ib qho yooj yim^TMMix (UA)
Cov lus qhia

Nta & qhov zoo

■Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.

■ Cov qauv xav tau me me, tsawg li 5mg nas cov ntaub so ntswg lossis 2-5mm nas tus Tsov tus tw tuaj yeem kuaj tau.

■ Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab kev sib tsoo, thiab kev ua haujlwm yooj yim.

■ Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv.

■ Anti-pollution PCR system 2×M1-PCR yooj yim^TMMix (UNG), uas zoo tshem tawm cov pa phem los ntawm PCR cov khoom thiab xyuas kom meej qhov tseeb thiab qhov tseeb ntawm amplification.