Viral RNA Isolation Kit rau Viral RNA Purification los ntawm Plasma, Serum, thiab Lwm Cov Qauv
Kit Description:
No.RE-02011/02014
Rau kev ua kom huv ntawm tus kab mob RNA los ntawm cov ntshav ntshav, ntshav, cell-free lub cev kua, cell-culture supernatants.
Kev cais tawm sai sai thiab ntxuav cov kab mob RNA los ntawm cov qauv xws li ntshav, ntshav, cell-free lub cev kua thiab cell culture supernatants.
-Tsis tas yuav txhawj txog RNA degradation.Tag nrho cov khoom siv yog RNase-Free
- Yooj yim - txhua qhov haujlwm ua tiav ntawm chav tsev kub
-Fast-kev ua haujlwm tuaj yeem ua tiav hauv 20 feeb
-High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv RNA zoo
-Safe-tsis muaj cov organic reagent siv
-Cov qauv ua qauv loj - txog li 200μl cov qauv tuaj yeem ua tiav txhua lub sijhawm.
-High zoo - lub purified RNA yog heev ntshiab, tsis muaj protein ntau thiab lwm yam impurities, thiab muaj peev xwm ua tau raws li ntau yam downstream kev siv.
Cov lus piav qhia
Cov khoom siv siv cov kab rov tav thiab cov qauv tsim los ntawm Foregene, uas tuaj yeem tshem tawm cov purity siab thiab cov kab mob zoo RNA los ntawm cov qauv xws li ntshav, ntshav, cell-free lub cev kua, thiab cell culture supernatant.Cov khoom siv tshwj xeeb ntxiv Linear Acrylamide, uas tuaj yeem ntes tau me me ntawm RNA los ntawm cov qauv.RNA-Tsuas Kem tuaj yeem khi RNA zoo.Cov khoom siv tuaj yeem ua cov qauv ntau ntawm tib lub sijhawm.
Tag nrho cov khoom siv tsis muaj RNase, yog li RNA purified yuav tsis degraded.Buffer viRW1 thiab Buffer viRW2 tuaj yeem ua kom ntseeg tau tias cov kab mob kis tau los ntawm nucleic acid tsis muaj protein ntau, nuclease lossis lwm yam impurities, uas tuaj yeem siv ncaj qha rau kev sim hauv dej molecular biology.
Specifications
50 Preps, 200 Preps
Cov khoom siv
| Linear Acrylamide |
| Buffer viRL |
| Buffer viRW1, Tsis muaj viRW2 |
| RNase-Dawb ddH2O |
| RNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
-Tsis tas yuav txhawj txog RNA degradation.Tag nrho cov khoom siv yog RNase-Free
- Yooj yim - txhua qhov haujlwm ua tiav ntawm chav tsev kub
-Fast-kev ua haujlwm tuaj yeem ua tiav hauv 20 feeb
-High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv RNA zoo
-Safe-tsis muaj cov organic reagent siv
-Cov qauv ua qauv loj - txog li 200μl cov qauv tuaj yeem ua tiav txhua lub sijhawm.
-High zoo - lub purified RNA yog heev ntshiab, tsis muaj protein ntau thiab lwm yam impurities, thiab muaj peev xwm ua tau raws li ntau yam downstream kev siv.
Cov khoom siv parameter
Khoom siv:
Nws yog qhov tsim nyog rau kev rho tawm thiab ntxuav cov kab mob RNA hauv cov qauv xws li ntshav, ntshav, cell-free lub cev kua thiab cell culture supernatant.
Kev khiav haujlwm
Cia tej yam kev mob
- Cov khoom siv tuaj yeem khaws cia rau 24 lub hlis ntawm chav tsev kub (15-25 ℃), lossis 2-8 ℃ rau lub sijhawm ntev.
-Linear Acrylamide kua tuaj yeem khaws cia hauv chav sov li 7 hnub.Tom qab tau txais cov khoom siv, thov muab cov tshuaj Linear Acrylamide tawm thiab khaws cia ntawm -20 ℃.
-Tom qab ntxiv Linear Acrylamide rau Buffer viRL, nws tuaj yeem khaws cia ntawm 2-8 ℃ txog li 48 teev.Thov siv cov tshuaj npaj tshiab.
Cov lus qhia rau kev txheeb xyuas qhov teeb meem
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Tsis muaj RNA tuaj yeem muab rho tawm lossis cov txiaj ntsig ntawm nucleic acid tsawg
Feem ntau muaj ntau yam uas cuam tshuam rau kev rov ua haujlwm zoo, xws li: qauv RNA cov ntsiab lus, txoj kev ua haujlwm, qhov ntim elution, thiab lwm yam.
Analysis ntawm common ua:
1.Ice da dej los yog qhov kub thiab txias (4 ° C) centrifugation thaum ua haujlwm.
Tswv yim: Chav ntsuas kub (15-25 ° C) ua haujlwm, tsis txhob da dej da dej thiab kub centrifuge.
2. Cov qauv khaws cia tsis raug lossis cov qauv khaws cia ntev dhau.
Tswv yim: Khaws cov qauv ntawm -80 ° C los yog khov hauv cov kua nitrogen, thiab tsis txhob rov siv dua khov-thaw;sim siv cov qauv sau tshiab rau RNA rho tawm.
3.Cov qauv lysis tsis txaus
Cov lus pom zoo: Thov xyuas kom meej tias cov qauv thiab cov kev daws teeb meem ua haujlwm (Linear Acrylamide) tau muab sib xyaw kom zoo thiab incubated rau 10 min ntawm chav tsev kub (15-25 ° C)
4.Qhov eluent tau ntxiv tsis raug
Kev pom zoo: Nco ntsoov tias RNase-Free ddH2O ntxiv rau hauv nruab nrab ntawm daim nyias nyias ntawm kab purification
5.Improper ntim ntawm anhydrous ethanol hauv Buffer viRW2
Tswv yim: Thov ua raws li cov lus qhia, ntxiv qhov tseeb ntim ntawm anhydrous ethanol rau Buffer viRW2 thiab sib tov zoo ua ntej siv cov khoom siv.
6. Cov qauv siv tsis raug.
Tswv yim: 200µl ntawm cov qauv ib 500μl ntawm Buffer viRL.Cov qauv ntim ntau dhau yuav ua rau txo RNA rho tawm tus nqi.
7.Improper elution ntim lossis tsis tiav elution.
Tswv yim: Lub ntim eluent ntawm purification kem yog 30-50μl;Yog tias cov nyhuv elution tsis txaus siab, nws raug nquahu kom ntxiv pre-rhuab RNase-Free ddH2O thiab ncua sij hawm tso rau hauv chav tsev kub, xws li 5-10min
8.Purification kem muaj ethanol residue tom qab yaug hauv Buffer viRW2.
Cov lus pom zoo: Yog tias ethanol tseem tseem nyob tom qab yaug tawm hauv chav nyob hauv chav tsev rau 5min, colation-raj centrifugation rau tag nrho cov ethanol.
Lub degradation ntawm purified RNA molecules
Qhov zoo ntawm RNA purified muaj feem xyuam nrog cov khoom xws li cov qauv cia, RNase paug, thiab kev ua haujlwm.
Analysis ntawm common ua:
1.Cov qauv sau tseg tsis tau txais kev cawmdim raws sijhawm.
Cov lus qhia: Yog tias tus qauv tsis siv sijhawm tom qab sau, thov khaws cia ntawm -80 ℃ lossis kua nitrogen tam sim.Rau kev rho tawm ntawm RNA molecules, sim siv cov qauv sau tshiab thaum twg los tau.
2.Cov qauv sau tau khov thiab thawing ntau zaus.
Tswv yim: Tsis txhob rov ua kom khov thiab thawing (tsis pub ntau tshaj ib zaug) thaum lub sij hawm coj mus kuaj thiab cia, txwv tsis pub cov tawm los ntawm nucleic acid yuav txo.
3.RNase tau qhia hauv chav ua haujlwm lossis tsis muaj hnab looj tes pov tseg, qhov ncauj qhov ntswg, thiab lwm yam.
Tswv yim: Kev rho tawm ntawm RNA molecules kev sim ua tau zoo tshaj plaws hauv chav ua haujlwm RNA cais, thiab cov lus sim raug ntxuav ua ntej kev sim.Hnav hnab looj tes thiab lub qhov ncauj qhov ntswg pov tseg thaum lub sij hawm sim kom tsis txhob RNA degradation los ntawm RNase qhia.
4.Cov reagent muaj paug los ntawm RNase thaum siv.
Tswv yim: Hloov tshiab Viral RNA Isolation Kit rau lwm yam kev sim.
5.The RNase paug ntawm cov raj centrifuge, cov lus qhia pipette, thiab lwm yam. Cov lus qhia: Xyuas kom tseeb tias cov raj centrifuge, cov lus qhia pipette, thiab pipettes yog tag nrho RNase-Free.
Cov purified RNA molecules cuam tshuam cov kev sim hauv qab
RNA molecules purified los ntawm kab purification yuav cuam tshuam cov kev sim downstream yog tias muaj ntsev ions ntau dhau los yog cov proteins, xws li: thim rov qab, Northern Blot, thiab lwm yam.
1.Muaj cov ntsev ions ntxiv hauv cov eluted RNA molecules.
Kev pom zoo: Xyuas kom tseeb tias qhov ntim ntawm cov dej tsis muaj dej ethanol tau muab ntxiv rau Buffer viRW2, thiab ntxuav cov purification kem ob zaug raws li qhov tseeb centrifugation ceev ntawm cov lus qhia ua haujlwm; Yog tias tseem muaj ntsev ions ntxiv, koj tuaj yeem ntxiv Buffer viRW2 rau hauv kem purification, thiab tawm hauv chav sov li 5 feeb.Tom qab ntawd ua centrifugation kom tshem tawm cov ntsev ions paug rau qhov loj tshaj plaws
2.Muaj ethanol nyob rau hauv eluted RNA molecules
Cov lus qhia: ib zaug lees paub tias cov kab huv huv tau muab yaug los ntawm Buffer viRW2, ua cov raj khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob.Yog tias tseem muaj ethanol tseem tshuav, nws tuaj yeem tso tseg rau 5 feeb ntawm chav tsev kub tom qab lub raj khoob khoob khoob kom tshem tawm cov ethanol seem mus rau qhov loj tshaj plaws.
Phau ntawv qhia:
Viral RNA Isolation Kit Instruction Manual
