Foregene DNA Identification System Y Plus (Tsis-Extraction)
Kit Description:
ForegeneDNA identification system Y Plus siv 6-xim fluorescent labeling tshuab rau amplify 20 Y chromosome core loci, 15 nyiam loci, 6 lwm loci thiab 3 Y indel loci nyob rau hauv ib lub sij hawm.
Kev piav qhia
ForegeneDNA identification system Y Plus siv 6-xim fluorescent labeling tshuab rau amplify 20 Y chromosome core loci, 15 nyiam loci, 6 lwm loci thiab 3 Y indel loci nyob rau hauv ib lub sij hawm.
Cov ntsiab lus
Amplification system npaj
Covkev npaj muaj pes tsawg leegntawm standard(tsis muaj extraction)amplification system
| Ccov omponents | 25 ua m lqhov system (ua m l) | 10μl system (μl) | |
| Master Mix | 4 × Master MixVII | 6.25 ib | 2.5 |
| 5 × Y Plus Primer Mix | 5.0 | 2.0 | |
| Deionized dej | 13.75 Nws | 5.5 | |
| Blood stain | Txoj kab uas hla 1.2mm | Txoj kab uas hla 1.2mm | |
| Total cov tshuaj tiv thaiv ntim | 25 ua m l | 10 ua m l | |
Kev txheeb xyuas cov ntaub ntawv
Annexed Graph 1:Allelic Ladder Typing Map
Annexed Graph 2:DNA typing standard 9948 typingdaim ntawv qhia
Reagent Cia
1. Thov khaws cia hauv qab -20 ° C (Yog tias tsis siv ib ntus), tom qab tau txais cov khoom siv khov rau hauv cov dej khov qhuav lossis gel ice packs.
2. Thov khaws cov tshuaj tiv thaiv ua ntej 4 × premix VII ntawm -20 ° C, tom qab cov khoom siv raug tshem tawm, thiab lwm cov tshuaj tiv thaiv ua ntej yuav raug muab khaws cia ntawm 4 ° C kom tsis txhob rov khov thiab thawing.Yog tias qhov ntau npaum li cas me me, nws raug pom zoo kom khaws cia ntawm -20 ° C tom qab aliquoting.
3. Khaws cov khoom tom qab cov tshuaj tiv thaiv ntawm 4 ° C, tsis txhob rov khov thiab thawing, thiab tsis txhob kov cov reagents ua ntej cov tshuaj tiv thaiv kom tsis txhob muaj kab mob.
