Ntshav RNA Isolation Kit
Kit Description:
Cat. No.RE-04011/04013
Rau tag nrho RNA purification los ntawm tag nrho cov ntshav 104 ≤ Cov Ntshav Dawb ≤ 107
Kev cais tawm sai sai thiab ntxuav cov ntshav RNA los ntawm cov qe ntshav dawb.
-Tsis tas yuav txhawj txog RNA degradation.Tag nrho cov khoom siv yog RNase-Free
- Yooj yim - txhua qhov haujlwm ua tiav ntawm chav tsev kub
-Fast-kev ua haujlwm tuaj yeem ua tiav hauv 20 feeb
-High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv RNA zoo
-Safe-tsis muaj cov organic reagent siv
-Cov qauv ua qauv loj - txog li 200μl cov qauv tuaj yeem ua tiav txhua lub sijhawm.
-High zoo - lub purified RNA yog heev ntshiab, tsis muaj protein ntau thiab lwm yam impurities, thiab muaj peev xwm ua tau raws li ntau yam downstream kev siv.
Cov lus piav qhia
Cov khoom siv tau txais cov kab ke thiab cov qauv tsim los ntawm peb lub tuam txhab, uas tuaj yeem tshem tawm cov purity siab thiab zoo tag nrho RNA los ntawm cov ntshav tag nrho.Cov khoom siv muab cov qe ntshav liab lysate (Buffer RCL), uas tuaj yeem ceev thiab ua tau zoo lyse cov qe ntshav liab thiab khaws cov qe ntshav dawb.Qhov zoo DNA-Tshuaj Colunm tau yooj yim cais cov supernatant thiab cell lysates thiab adsorb thiab tshem tawm genomic DNA.Kev ua haujlwm yog yooj yim thiab txuag lub sijhawm;RNA-tsuas yog kab tuaj yeem khi RNA zoo, thiab nrog cov qauv tshwj xeeb, nws tuaj yeem ua cov qauv ntau tib lub sijhawm.
Tag nrho cov kab ke RNase-Free ua rau rho tawm RNA tsis-degradable;Buffer RW1 thiab Buffer RW2 tsis siv lub tshuab ntxhua khaub ncaws ua rau RNA tau txais tsis muaj protein ntau, DNA, ions thiab organic compound pollution.
Cov khoom siv
| Blood Total RNA Isolation Kit | ||
| Cov khoom siv muaj pes tsawg leeg | TSI-04011 | TSI-04013 |
| 50x ua | 200 x | |
| Tsis Siv Neeg RCL (10 ×) | 52.5ml ib | 210 ml yog ' |
| Tsis muaj BRL1 * | 30 ml | 120 ml |
| Tsis muaj BRL2 | 18ml ib | 66ml ib |
| Buffer RW1* | 25ml ib | 100 ml |
| Buffer RW2 | 24ml ib | 96ml ib |
| RNase-Dawb ddH2 O | 10 ml | 40 ml |
| RNA nkaus xwb | 50 sets | 200 sets |
| DNA-Tswj Kem | 50 sets | 200 sets |
| phau ntawv | 1 luam | 1 luam |
Nta & qhov zoo
-Tsis tas yuav txhawj txog RNA degradation.Tag nrho cov khoom siv yog RNase-Free.
- Yooj yim - txhua qhov haujlwm ua tiav ntawm chav tsev kub.
-Fast-kev ua haujlwm tuaj yeem ua tiav hauv 20 feeb.
-High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv RNA zoo.
-Safe - tsis muaj cov organic reagent siv.
-Cov qauv ua qauv loj - txog li 200μl cov qauv tuaj yeem ua tiav txhua lub sijhawm.
-High zoo - lub purified RNA yog heev ntshiab, tsis muaj protein ntau thiab lwm yam impurities, thiab muaj peev xwm ua tau raws li ntau yam downstream kev siv.
Cov khoom siv parameter
Khoom siv:
Nws yog tsim nyog rau kev rho tawm thiab ua kom huv ntawm tag nrho RNA los ntawm mammalian tag nrho cov ntshav.
Kev khiav haujlwm
Cia tej yam kev mob
Buffer RCL (10 ×) yuav tsum khaws cia ntawm 2-8 ℃;Lwm yam khoom siv ntawm cov khoom siv tuaj yeem khaws cia hauv chav tsev kub (15-25 ℃) hauv qhov chaw qhuav, thiab tuaj yeem khaws cia rau 12 lub hlis.Buffer BRL1 tuaj yeem khaws cia ntawm 4 ℃ rau 1 lub hlis tom qab ntxiv β-mercaptoethanol (optional).
Lus Cim: Yog tias khaws cia ntawm qhov kub thiab txias, qhov kev daws teeb meem yuav ua rau nag lossis daus.Nco ntsoov muab cov tshuaj tso rau hauv cov khoom siv ntawm chav tsev kub rau lub sijhawm ua ntej siv.Yog tias tsim nyog, preheat nws hauv 37 ° C dej da dej rau 10 feeb kom yaj cov dej nag, thiab sib tov zoo ua ntej siv.
Cov lus qhia rau kev txheeb xyuas qhov teeb meem
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Tsis muaj RNA tuaj yeem muab rho tawm lossis cov txiaj ntsig ntawm nucleic acid tsawg
Feem ntau muaj ntau yam uas cuam tshuam rau kev rov ua haujlwm zoo, xws li: qauv RNA cov ntsiab lus, txoj kev ua haujlwm, qhov ntim elution, thiab lwm yam.
Analysis ntawm common ua:
1.Ice da dej los yog qhov kub thiab txias (4 ° C) centrifugation thaum ua haujlwm.
Tswv yim: Chav ntsuas kub (15-25 ° C) ua haujlwm, tsis txhob da dej da dej thiab kub centrifuge.
2. Cov qauv khaws cia tsis raug lossis cov qauv khaws cia ntev dhau.
Tswv yim: Khaws cov qauv ntawm -80 ° C los yog khov hauv cov kua nitrogen, thiab tsis txhob rov siv dua khov-thaw;sim siv cov qauv sau tshiab rau RNA rho tawm.
3.Cov qauv lysis tsis txaus
Cov lus pom zoo: Thov xyuas kom meej tias cov qauv thiab cov kev daws teeb meem ua haujlwm (Linear Acrylamide) tau muab sib xyaw kom zoo thiab incubated rau 10 min ntawm chav tsev kub (15-25 ° C)
4.Qhov eluent tau ntxiv tsis raug
Kev pom zoo: Nco ntsoov tias RNase-Free ddH2O ntxiv rau hauv nruab nrab ntawm daim nyias nyias ntawm kab purification
5.Improper ntim ntawm anhydrous ethanol hauv Buffer viRW2
Tswv yim: Thov ua raws li cov lus qhia, ntxiv qhov tseeb ntim ntawm anhydrous ethanol rau Buffer viRW2 thiab sib tov zoo ua ntej siv cov khoom siv.
6. Cov qauv siv tsis raug.
Tswv yim: 200µl ntawm cov qauv ib 500μl ntawm Buffer viRL.Cov qauv ntim ntau dhau yuav ua rau txo RNA rho tawm tus nqi.
7.Improper elution ntim lossis tsis tiav elution.
Tswv yim: Lub ntim eluent ntawm purification kem yog 30-50μl;Yog tias cov nyhuv elution tsis txaus siab, nws raug nquahu kom ntxiv pre-rhuab RNase-Free ddH2O thiab ncua sij hawm tso rau hauv chav tsev kub, xws li 5-10min
8.Purification kem muaj ethanol residue tom qab yaug hauv Buffer viRW2.
Cov lus pom zoo: Yog tias ethanol tseem tseem nyob tom qab yaug tawm hauv chav nyob hauv chav tsev rau 5min, colation-raj centrifugation rau tag nrho cov ethanol.
Lub degradation ntawm purified RNA molecules
Qhov zoo ntawm RNA purified muaj feem xyuam nrog cov khoom xws li cov qauv cia, RNase paug, thiab kev ua haujlwm.
Analysis ntawm common ua:
1.Cov qauv sau tseg tsis tau txais kev cawmdim raws sijhawm.
Cov lus qhia: Yog tias tus qauv tsis siv sijhawm tom qab sau, thov khaws cia ntawm -80 ℃ lossis kua nitrogen tam sim.Rau kev rho tawm ntawm RNA molecules, sim siv cov qauv sau tshiab thaum twg los tau.
2.Cov qauv sau tau khov thiab thawing ntau zaus.
Tswv yim: Tsis txhob rov ua kom khov thiab thawing (tsis pub ntau tshaj ib zaug) thaum lub sij hawm coj mus kuaj thiab cia, txwv tsis pub cov tawm los ntawm nucleic acid yuav txo.
3.RNase tau qhia hauv chav ua haujlwm lossis tsis muaj hnab looj tes pov tseg, qhov ncauj qhov ntswg, thiab lwm yam.
Tswv yim: Kev rho tawm ntawm RNA molecules kev sim ua tau zoo tshaj plaws hauv chav ua haujlwm RNA cais, thiab cov lus sim raug ntxuav ua ntej kev sim.Hnav hnab looj tes thiab lub qhov ncauj qhov ntswg pov tseg thaum lub sij hawm sim kom tsis txhob RNA degradation los ntawm RNase qhia.
4.Cov reagent muaj paug los ntawm RNase thaum siv.
Tswv yim: Hloov tshiab Viral RNA Isolation Kit rau lwm yam kev sim.
5.The RNase paug ntawm cov raj centrifuge, cov lus qhia pipette, thiab lwm yam. Cov lus qhia: Xyuas kom tseeb tias cov raj centrifuge, cov lus qhia pipette, thiab pipettes yog tag nrho RNase-Free.
Cov purified RNA molecules cuam tshuam cov kev sim hauv qab
RNA molecules purified los ntawm kab purification yuav cuam tshuam cov kev sim downstream yog tias muaj ntsev ions ntau dhau los yog cov proteins, xws li: thim rov qab, Northern Blot, thiab lwm yam.
1.Muaj cov ntsev ions ntxiv hauv cov eluted RNA molecules.
Kev pom zoo: Xyuas kom tseeb tias qhov ntim ntawm cov dej tsis muaj dej ethanol tau muab ntxiv rau Buffer viRW2, thiab ntxuav cov purification kem ob zaug raws li qhov tseeb centrifugation ceev ntawm cov lus qhia ua haujlwm; Yog tias tseem muaj ntsev ions ntxiv, koj tuaj yeem ntxiv Buffer viRW2 rau hauv kem purification, thiab tawm hauv chav sov li 5 feeb.Tom qab ntawd ua centrifugation kom tshem tawm cov ntsev ions paug rau qhov loj tshaj plaws
2.Muaj ethanol nyob rau hauv eluted RNA molecules
Cov lus qhia: ib zaug lees paub tias cov kab huv huv tau muab yaug los ntawm Buffer viRW2, ua cov raj khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob khoob.Yog tias tseem muaj ethanol tseem tshuav, nws tuaj yeem tso tseg rau 5 feeb ntawm chav tsev kub tom qab lub raj khoob khoob khoob kom tshem tawm cov ethanol seem mus rau qhov loj tshaj plaws.
Phau ntawv qhia:
Viral RNA Isolation Kit Instruction Manual
