Foregene DNA Identification System 30 AC (Case)
Kit Description:
Foregene DNA identification system30A-C siv 6-xim fluorescent labeling tshuab rauua 29autosomal uaSTR loci ib, 1 Amel Locus, 1 Yindellocus, thiab1 tib neeg hom-specific Sexual sites, 2internal zoo tswj chaw, siv los amplify lubDNAtemplate muab rho tawm thiab purified los ntawmCcov ntaub ntawv uas.
Kev piav qhia
Foregene DNA identification system30A-C siv 6-xim fluorescent labeling tshuab rauua 29autosomal uaSTR loci ib, 1 Amel Locus, 1 Yindellocus, thiab1 tib neeg hom-specific Sexual sites, 2internal zoo tswj chaw, siv los amplify lubDNAtemplate muab rho tawm thiab purified los ntawmCcov ntaub ntawv uas.
Cov ntsiab lus
Amplification system npaj
Table 2: Kev Npaj Cov Txheej Txheem ntawm kev rho tawm thiab kev ua kom nrov nrov
|
| Ccov omponents | 25 ul system (µl) | 10μl system (μl) |
| Master Mix | 2.5 × PremixCⅡ | 10.0 | 4.0 |
| 5 x 30 AC Primer Mix | 5.0 | 2.0 | |
| DNA template | 2.5—10 | 1—4 | |
| Deionized dej | Ua kom muaj cov tshuaj tiv thaiv ntim ntawm 25ua m l | Ua kom muaj cov tshuaj tiv thaiv ntim ntawm10 l m |
Kev txheeb xyuas cov ntaub ntawv
Annexed Graph 1:Allelic Ladder Typing Map
Annexed Graph 2:DNA typing standard 9948 typingdaim ntawv qhia
Reagent Cia
1. Thov khaws cia hauv qab -20 ° C, tom qab tau txais cov khoom siv khov rau hauv cov dej khov qhuav lossis gel ice packs.
2. Thov khaws cov tshuaj tiv thaiv ua ntej 2.5 × premix CII ntawm -20 ° C tom qab cov khoom siv raug tshem tawm, thiab cov khoom siv ua ntej cov tshuaj tiv thaiv yog khaws cia ntawm 4 ° C kom tsis txhob rov khov thiab thawing.Yog tias qhov ntau npaum li cas me me, nws raug pom zoo kom khaws cia ntawm -20 ° C tom qab aliquoting.
3. Khaws cov khoom tom qab cov tshuaj tiv thaiv ntawm 4 ° C, tsis txhob rov khov thiab thawing, thiab tsis txhob kov cov reagents ua ntej cov tshuaj tiv thaiv kom tsis txhob muaj kab mob.
