Foregene DNA Identification System 30A (Non-Extraction)
Kit Description:
FOREGENE DNA identification system 30A siv 6-xim fluorescent labeling tshuab rauua 29autosomal uaSTR loci, ibAmel locus thiab ib Yindel locus nyob rau hauv ib lub sij hawm, thiab muaj peev xwm ncaj qha amplify lim ntawv, FTAdaim npav, paj rwb swabs, qaub ncaug cards,thiab mouth swb.
Kev piav qhia
FOREGENE DNA identification system 30A siv 6-xim fluorescent labeling tshuab rauua 29autosomal uaSTR loci, ibAmel locus thiab ib Yindel locus nyob rau hauv ib lub sij hawm, thiab muaj peev xwm ncaj qha amplify lim ntawv, FTAdaim npav, paj rwb swabs, qaub ncaug cards,thiab mouth swb.
Cov ntsiab lus
Amplification System Npaj
Table 1: Kev npaj ua ke ntawm Standard (Extraction-free) amplification cov tshuaj tiv thaiv
|
| Ccov omponents | 25 Qhov system (μl) | 10 Qhov system (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 ib | 2.5 |
| 5 x 30 APrimer Mix | 5.0 | 2 | |
| Deionized dej | 13.75 Nws | 5.5 | |
| Blood stain | Txoj kab uas hla 1.2mm | Txoj kab uas hla 1.2mm | |
| Total cov tshuaj tiv thaiv ntim | 25 ua m l | 10 ua m l | |
Kev txheeb xyuas cov ntaub ntawv
Annexed Graph 1:Allelic Ladder Typing Map
Annexed Graph 2:DNA typing standard 9948 typingdaim ntawv qhia
Reagent Cia
1. Thov khaws cia hauv qab -20 ° C, tom qab tau txais cov khoom siv khov rau hauv cov dej khov qhuav lossis gel ice packs.
2. Thov khaws cov tshuaj tiv thaiv ua ntej 4 × premix VII ntawm -20 ° C, tom qab cov khoom siv tawm los siv, thiab khaws lwm cov tshuaj tiv thaiv ua ntej ua ntej ntawm 4 ° C kom tsis txhob rov khov thiab thawing.Yog tias qhov ntau npaum li cas me me, nws raug pom zoo kom khaws cia ntawm -20 ° C tom qab aliquoting,
3. Khaws cov khoom tom qab cov tshuaj tiv thaiv ntawm 4 ° C, tsis txhob rov khov thiab thawing, thiab tsis txhob kov cov reagents ua ntej cov tshuaj tiv thaiv kom tsis txhob muaj kab mob.
