Foregene DNA Identification System 25A-C (Case)
Kit Description:
Foregene DNA Identification System 25A-C siv 6-xim fluorescence labeling thev naus laus zis los nthuav tawm 23 autosomal STR loci, ib qho Amel loci, ib Yindel loci thiab ob qho kev tswj xyuas sab hauv zoo nyob rau ib lub sijhawm rau kev nthuav dav ntawm DNA qauv muab rho tawm thiab huv los ntawm cov ntaub ntawv kuaj.
Kev piav qhia
Foregene DNA Identification System 25A-C siv 6-xim fluorescence labeling thev naus laus zis los nthuav tawm 23 autosomal STR loci, ib qho Amel loci, ib Yindel loci thiab ob qho kev tswj xyuas sab hauv zoo nyob rau ib lub sijhawm rau kev nthuav dav ntawm DNA qauv muab rho tawm thiab huv los ntawm cov ntaub ntawv kuaj.
Cov ntsiab lus
Amplification system npaj
Kev Npaj Ua Haujlwm ntawm Cov Txheej Txheem Extraction thiab Amplification System
| Cheebtsam | 10μl system (μl) | |
| Master Mix | 2.5 × PCR Premix C Ⅰ | 4.0 |
| 10 × 25A-Cprimer Mix | 1.0 | |
| DNA template | 1—5 | |
| Deionized dej | Ua kom muaj cov tshuaj tiv thaiv ntim ntawm 10μl |
Kev txheeb xyuas cov ntaub ntawv
Annexed Graph 1:Ladder genotyping ntawm allele
Annexed Graph 2:DNA typing standard 9948 typingdaim ntawv qhia
Reagent Cia
1. Tom qab tau txais cov khoom siv, thov khaws cia hauv qab -20 ℃ yog tias koj tsis siv rau lub sijhawm.
2. Tom qab cov khoom siv tawm thiab siv, cov tshuaj tiv thaiv ua ntej PCR premixed tov yog khaws cia ntawm -20 ℃, thiab cov khoom siv ua ntej cov tshuaj tiv thaiv yog khaws cia ntawm 4 ℃ kom tsis txhob rov khov-thaw.Yog tias qhov ntau npaum li cas me me, nws raug pom zoo kom khaws cia ntawm -20 ° C tom qab ntim.
3. Tom qab cov tshuaj tiv thaiv, cov khoom raug khaws cia ntawm 4 ℃ kom tsis txhob rov khov-thaw.Tsis txhob kov cov tshuaj tiv thaiv ua ntej kom tsis txhob muaj kuab paug.
