Foregene DNA Identification System 27Y
Kev piav qhia
Foregene DNA Identification System 27Y siv 6-xim fluorescent labeling tshuab los ua kom nrov 27 Y-STR loci ib zaug.Xws li: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS3189, DYS38 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.
Cov khoom siv muaj peev xwm tiv thaiv kev tiv thaiv zoo, thiab tuaj yeem txuas ncaj qha rau cov ntawv lim, FTA card, paj rwb swab, daim ntawv qaub ncaug, qhov ncauj qhov ncauj, thiab tseem tuaj yeem siv los rho tawm thiab ntxuav cov qauv DNA los ntawm cov ntaub ntawv.
Cov ntsiab lus
Cov khoom siv tshuaj reagent | Khoom npe | Ntim (μl / tube) | Quantity (tube) |
PCR cov khoom siv ua ntej cov tshuaj tiv thaiv | 2 × PCR Reaction Master Mix | 1250 | 2 |
5 x 27Y Primer Mix | 500 | 2 | |
DNA Typing Standard 9948 | 20 | 2 | |
Deionized dej | 1700 | 2 | |
Tom qab PCR Reaction Kit | 27Y allele mix ntaiv | 20 | 2 |
Txiv kab ntxwv molecular hnyav sab hauv tus qauv ORG500 | 150 | 2 |
Kev txheeb xyuas cov ntaub ntawv
1. Qhib Gene Mapper ID software.Thaum siv cov khoom siv no thawj zaug, koj yuav tsum tau ntshuam panels, bin set, coj txoj kev tsom xam, thiab tus qauv loj (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 350, 30, 207, 0, 450, 490, 500).
2. Ntshuam cov ntaub ntawv electrophoresis, xaiv cov kev txheeb xyuas tsis sib xws xws li vaj huam sib luag, kev soj ntsuam txoj kev, thiab cov qauv loj, thiab hloov cov qauv ntaiv rau "Allelic Ladder" hauv kab "Sample Type" kem;pib txheeb xyuas cov ntaub ntawv.
Reagent Cia
1. Thov khaws cia hauv qab -20 ° C (yog tias tsis siv ib ntus), tom qab tau txais cov khoom siv khov rau hauv cov dej khov qhuav lossis cov gel ice packs.
2. Thov khaws cov khoom siv ntawm 4 ° C ua ntej cov tshuaj tiv thaiv kom tsis txhob rov khov thiab thawing, tom qab muab cov khoom siv tawm, ;enzyme yuav tsum tau khaws cia ntawm -20 ° C.
2. Tom qab cov tshuaj tiv thaiv, cov khoom siv (xws li molecular hnyav nyob rau hauv tus qauv thiab ntaiv) yuav tsum tau muab cia rau hauv "electrophoresis nrhiav kom tau chav" ntawm 4 ° C, tsis txhob rov qab khov thiab thawing, thiab tsis txhob sib cuag nrog cov khoom siv ua ntej cov tshuaj tiv thaiv kom tsis txhob muaj kab mob.