Foregene DNA Identification System 20A (Dawb DNA Extraction)
Kit Description:
Foregene DNA identification system 20A siv tsib-xim fluorescent labeling tshuab rau ib txhij amplify 19 STR loci thiab 1 sex loci nyob rau hauv ib lub raj.Thaum npog 13 CODIS core loci, 20 core loci ua tau raws li qhov xav tau ntawm Ministry of Public Security rau autosomal STR cov khoom siv.Nws yog tus txiv neej sab xis ntawm forensic DNA database kev tsim kho thiab kev txiav txim plaub ntug kev txheeb xyuas.
Kev piav qhia
Foregene DNA identification system 20A siv tsib-xim fluorescent labeling tshuab rau ib txhij amplify 19 STR loci thiab 1 sex loci nyob rau hauv ib lub raj.Thaum npog 13 CODIS core loci, 20 core loci ua tau raws li qhov xav tau ntawm Ministry of Public Security rau autosomal STR cov khoom siv.Nws yog tus txiv neej sab xis ntawm forensic DNA database kev tsim kho thiab kev txiav txim plaub ntug kev txheeb xyuas.
Cov ntsiab lus
| Kit Componet | Componet npe | Volume (μl / tube) | Quantity (tube) |
| PCR Pre-reaction reagent | 2.5 × PCR cov tshuaj tiv thaiv tsis zoo Ⅲ | 1000 | 2 |
| 5 × 20A primer mix | 500 | 2 | |
| Tswj DNA 9947A (1ng / μl) | 25 | 1 | |
| Deionized dej | 1700 | 2 | |
| Cov Polymerase | Polymerase Ⅲ | 40 | 2 |
| PCR Rear-reaction reagent | Allelic ntaiv 20A | 40 | 1 |
| Molecular hnyav Internal standardORG 500 | 150 | 2 | |
| Nco tseg | |||
Kev txheeb xyuas cov ntaub ntawv
1. Qhib Genemapper ID software.Thaum siv cov khoom no thawj zaug, koj yuav tsum tau import cov panels, bin set, coj txoj kev tsom xam, thiab tus qauv loj (ORG500: 65, 70, 80, 100, 120, 140, 160, 180, 200, 225, 250, 30, 20, 30, 200, , 450, 490, 500)
2. Ntshuam cov ntaub ntawv electrophoresis, xaiv cov kev txheeb xyuas tsis sib xws xws li vaj huam sib luag, kev soj ntsuam txoj kev, thiab cov qauv loj, thiab hloov cov qauv ntaiv rau "Allelic Ladder" hauv kab "Sample Type" kem;pib txheeb xyuas cov ntaub ntawv.
Reagent Cia
1. Tom qab tau txais cov khoom siv khov rau hauv cov dej khov qhuav lossis cov pob yas yas, yog tias nws tsis siv ib ntus, thov khaws cia hauv qab -20 ° C rau lub sijhawm ntev.
2. Tom qab cov khoom siv tawm thiab siv, cov khoom siv yuav tsum muab khaws cia ntawm 4 ° C ua ntej cov tshuaj tiv thaiv kom tsis txhob rov khov thiab thawing;Taq enzyme yuav tsum khaws cia ntawm -20 ° C.
3. Tom qab cov tshuaj tiv thaiv, cov khoom siv (xws li molecular hnyav nyob rau hauv tus qauv thiab ntaiv) yuav tsum tau muab cia rau hauv "electrophoresis nrhiav kom tau chav" ntawm 4 ° C, tsis txhob rov qab khov thiab thawing, thiab tsis txhob sib cuag nrog cov khoom siv ua ntej cov tshuaj tiv thaiv kom tsis txhob muaj kab mob.
