Foregene DNA Identification System 38Y-C (Case)
Kit Description:
Foregene DNA identification system 38Y-C siv 6-xim fluorescent labeling tshuab los ua kom nrov 38 Y chromosome STR loci, 3 Y-Indel qhov chawthiabob sab hauv kev tswj xyuas qhov zoo ntawm ib lub sijhawmrau amplification Case cov qauv muab rho tawm los ntawm purified DNA templates.
Kev piav qhia
Foregene DNA identification system 38Y-C siv 6-xim fluorescent labeling tshuab los ua kom nrov 38 Y chromosome STR loci, 3 Y-Indel qhov chawthiabob sab hauv kev tswj xyuas qhov zoo ntawm ib lub sijhawmrau amplification Case cov qauv muab rho tawm los ntawm purified DNA templates.
Cov ntsiab lus
Amplification system npaj
Covkev npaj composition of Txujrho tawmthiab amplification system
| Master Mix | Ccov omponents | 10μl system (μl) |
| 2.5 × PCR Master Mix C I | 4.0 | |
| 5 x 38Y-C Primer Mix | 2.0 | |
| DNA template | 1- 4 | |
| Deionized dej | Sau mus txog qhov ntim ntawm cov tshuaj tiv thaiv10 l m |
Kev txheeb xyuas cov ntaub ntawv
Annexed Graph 1:Allelic Ladder Typing Map
Annexed Graph 2:DNA typing standard 9948 typingdaim ntawv qhia
Reagent Cia
1. Thov khaws cia hauv qab -20 ° C (yog tias tsis siv ib ntus), tom qab tau txais cov khoom siv khov rau hauv cov dej khov qhuav lossis cov gel ice packs.
2. Thov sotre cov tshuaj tiv thaiv ua ntej PCR master mix yog ntawm -20 ° C, tom qab cov khoom siv tau raug tshem tawm rau kev siv thiab cov khoom siv ua ntej cov tshuaj tiv thaiv yog khaws cia ntawm 4 ° C kom tsis txhob rov khov thiab thawing.Yog tias qhov ntau npaum li cas me me, nws raug pom zoo kom khaws cia ntawm -20 ° C tom qab aliquoting.
3. Khaws cov khoom tom qab cov tshuaj tiv thaiv ntawm 4 ° C, tsis txhob rov khov thiab thawing, thiab tsis txhob kov cov reagents ua ntej cov tshuaj tiv thaiv kom tsis txhob muaj kab mob.
