Foreasy HS Taq DNA Polymerase
Kit Description:
◮High Specification: Lub enzyme nrog siab kub pib ua haujlwm.
◮Ceev Amplification: 10 sec / kb.
◮High template adaptability: tuaj yeem siv los ua kom muaj txiaj ntsig zooGCtus nqithiabntau yam nyuaj-rau-amplify DNA template.
◮Strong fidelity: Lub fidelity yog 6 lub sij hawmof normal Taq Enzyme.
Kev piav qhia
Foreasy HS Taq DNA Polymerase yog ib tug tshiab Taq enzyme qhia nyob rau hauv Escherichia coli engineering kab mob los ntawm noob recombination technology.Tom qab cov enzyme tau kho nrog cov txheej txheem tshwj xeeb, nws's kev ua haujlwm yog inhibited ua ntej thermal activation, yog li inhibiting qhov tsis-specific amplification tshwm sim los ntawm cov non-specific annealing ntawm primers los yog primer dimers nyob rau hauv qis kub tej yam kev mob.Cov khoom no tsim nyog rau PCR React tshwj xeebion, M siab x PCR, siab GC cov ntsiab lus (> 60%),nrogtheem nrab qauvlos yog lwm yammuaj zog tom qab genomcov icsamplification thiab loj-scale genomcov icsamplification nrhiav tau.Lub enzyme muaj 5' → 3' DNA polymerase kev ua haujlwm thiab 5' → 3' exonuclease kev ua, tab sis tsis muaj 3' → 5' exonuclease kev ua.
Cov khoom siv
| Cheebtsam | TSI-01021 | TSI-01022 | TSI-01023 |
| Foreasy HS Taq DNA Polymerase (5 U / μL) | 5000 U (1 ml) | 50 KUB (10 mL) | 500 KUB (100 mL) |
| 2 × Taq Reaction Buffer | 25ml x 5 | 250ml x 5 | 500ml x 25 |
Nta & qhov zoo
- Siab tshwj xeeb: Cov enzyme nrog siab kub pib ua haujlwm.
- Ceev Amplification: 10 sec / kb.
- High template adaptability: tuaj yeem siv los ua kom muaj txiaj ntsig zooGCtus nqithiabntau yam nyuaj-rau-amplify DNA template.
- Kev ntseeg ruaj khov: Kev ncaj ncees yog 6 zaugof normal Taq Enzyme.
Daim ntawv thov Kit
- Ntau yam PCR / qPCR System thiab ncaj qha PCR system
- PCR Amplified DNA Fragment
- DNA cim
- DNA Sequence
- PCR plus A Tail
Kev Ua Haujlwm txhais
1U: Tus nqi ntawm enzyme xav tau los sib xyaw 10 nmol ntawmDNArau hauv acid-insoluble teeb meem siv activated salmon phev DNA li template / primer, 74 ° C, 30 feeb.
Kev mob tshwm sim
| Kub | Lub Sijhawm Tawm Tsam | Lub sijhawm voj voog |
| 37 ° C | 5 feeb | 1 |
| 94 ° C | 5 feeb | 1 |
| 94 ° C | 10 sec | 40 |
| 60 ° C | 10 sec |
Nco tseg:Rau 10 µL thiab 20 µL tshuab, ntxiv qhov sib npaug ntawm cov roj ntxhia yog tias lub thermal cycler tsis muaj lub hau kub.
PCR cov kev mob tshwm sim txawv nyob ntawm seb cov qauv ntawm cov qauv, primers, thiab lwm yam.Hauv kev ua haujlwm tshwj xeeb, nws yog qhov tsim nyog los tsim cov kev pom zoo ntawm cov tshuaj tiv thaiv, suav nrog qhov kub thiab txias, ncua sij hawm, thiab lwm yam, raws li cov xwm txheej tshwj xeeb xws li hom qauv, qhov loj ntawm lub hom phiaj fragment, lub hauv paus sib lawv liag ntawm cov khoom tawg, thiab GC cov ntsiab lus thiab ntev ntawm primer.
Cia
-20 ± 5 ° C rau 2 xyoos lossis ntawm -80 ° C rau kev khaws cia ntev.
Tsis muaj amplification signals
1.Cov Taq DNA Polymerase nyob rau hauv cov khoom siv poob nws cov kev ua si vim yog qhov khoom siv tsis raug los yog tas sij hawm ntawm cov khoom siv.
Kev pom zoo: Paub meej tias qhov chaw cia khoom ntawm cov khoom siv;rov ntxiv qhov tsim nyog ntawm Taq DNA Polymerase rau PCR system lossis yuav ib lub Sijhawm PCR tshiab rau cov kev sim cuam tshuam.
2.Muaj ntau cov inhibitors ntawm Taq DNA Polymerase hauv DNA template.
Tswv yim: Rov kho tus qauv los yog txo tus nqi ntawm cov qauv siv.
3.Qhov Mg2+ concentration tsis haum.
Kev pom zoo: Mg2 + concentration ntawm 2 × Tiag PCR Mix peb muab yog 3.5mM.Txawm li cas los xij, rau qee qhov tshwj xeeb primers thiab cov qauv, Mg2 + concentration yuav siab dua.Yog li ntawd, koj tuaj yeem ncaj qha ntxiv MgCl2 kom ua kom zoo dua Mg2 + concentration.Nws raug pom zoo kom nce Mg2 + 0.5mM txhua lub sijhawm rau kev ua kom zoo.
4.Lub PCR amplification tej yam kev mob tsis haum, thiab cov primer sib lawv liag los yog concentration yog tsis tsim nyog.
Cov lus qhia: paub meej tias qhov tseeb ntawm cov txheej txheem primer thiab cov primer tsis tau degraded;Yog hais tias lub teeb liab amplification tsis zoo, sim txo qhov kub thiab txias thiab kho cov primer concentration kom tsim nyog.
5.Tus qauv ntawm tus qauv yog tsawg lossis ntau dhau.
Kev pom zoo: Ua cov qauv linearization gradient dilution, thiab xaiv cov template concentration nrog qhov zoo tshaj plaws PCR nyhuv rau Real Time PCR sim.
NTC muaj tus nqi fluorescence siab dhau lawm
1.Reagent paug tshwm sim thaum lub sijhawm ua haujlwm.
Kev pom zoo: Hloov nrog cov tshuaj reagents tshiab rau kev sim PCR tiag tiag.
2.Contamination tshwm sim thaum lub sij hawm npaj ntawm cov tshuaj tiv thaiv PCR.
Kev pom zoo: Siv cov kev tiv thaiv tsim nyog thaum lub sijhawm ua haujlwm, xws li: hnav cov hnab looj tes latex, siv lub taub hau nrog lub lim dej, thiab lwm yam.
3.Cov primers yog degraded, thiab qhov degradation ntawm cov primers yuav ua rau tsis yog tshwj xeeb amplification.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov lawv nrog primers tshiab rau Real Time PCR thwmsim.
Primer dimer lossis tsis tshwj xeeb amplification
1.Qhov Mg2+ concentration tsis haum.
Kev pom zoo: Mg2 + concentration ntawm 2 × Tiag PCR EasyTM Mix peb muab yog 3.5 mM.Txawm li cas los xij, rau qee qhov tshwj xeeb primers thiab cov qauv, Mg2 + concentration yuav siab dua.Yog li ntawd, koj tuaj yeem ncaj qha ntxiv MgCl2 kom ua kom zoo dua Mg2 + concentration.Nws raug pom zoo kom nce Mg2 + 0.5mM txhua lub sijhawm rau kev ua kom zoo.
2.Lub PCR annealing kub yog tsawg dhau.
Tswv yim: Ua kom lub PCR annealing kub los ntawm 1 ℃ lossis 2 ℃ txhua zaus.
3.Cov khoom PCR ntev dhau lawm.
Kev pom zoo: Qhov ntev ntawm cov khoom PCR tiag tiag yuav tsum nyob nruab nrab ntawm 100-150bp, tsis ntau tshaj 500bp.
4.Cov primers yog degraded, thiab degradation ntawm cov primers yuav ua rau cov tsos ntawm tej yam amplification.
Tswv yim: Siv SDS-PAGE electrophoresis los xyuas seb cov primers puas tau degraded, thiab hloov lawv nrog primers tshiab rau Real Time PCR thwmsim.
5.Qhov system PCR tsis tsim nyog, lossis lub kaw lus me dhau.
Tswv yim: PCR cov tshuaj tiv thaiv me me dhau lawm yuav ua rau qhov tseeb ntawm kev kuaj pom yuav txo qis.Nws yog qhov zoo tshaj plaws los siv cov tshuaj tiv thaiv uas tau pom zoo los ntawm cov cuab yeej PCR ntau kom rov ua dua qhov kev sim PCR tiag tiag.
Tsis zoo repeatability ntawm quantitative values
1.Cov cuab yeej ua haujlwm tsis zoo.
Cov lus qhia: Tej zaum yuav muaj qhov yuam kev ntawm txhua lub PCR qhov ntawm lub ntsuas, uas ua rau tsis zoo rov tsim dua thaum tswj qhov kub thiab txias.Thov xyuas raws li cov lus qhia ntawm cov cuab yeej sib txuas.
2.Cov qauv purity tsis zoo.
Kev pom zoo: Cov qauv tsis huv yuav ua rau qhov tsis zoo ntawm qhov kev sim, uas suav nrog cov purity ntawm cov qauv thiab cov primers.Nws yog qhov zoo tshaj plaws los ntxuav cov qauv, thiab cov primers yog qhov zoo tshaj plaws purified los ntawm SDS-PAGE.
3.Qhov kev npaj PCR thiab lub sijhawm khaws cia ntev dhau lawm.
Cov lus qhia: Siv lub sijhawm tiag tiag PCR system rau PCR sim tam sim tom qab kev npaj, thiab tsis txhob tso nws tseg ntev dhau.
4.Lub PCR amplification tej yam kev mob tsis haum, thiab cov primer sib lawv liag los yog concentration yog tsis tsim nyog.
Cov lus qhia: paub meej tias qhov tseeb ntawm cov txheej txheem primer thiab cov primer tsis tau degraded;Yog hais tias lub teeb liab amplification tsis zoo, sim txo qhov kub thiab txias thiab kho cov primer concentration kom tsim nyog.
5.Qhov system PCR tsis tsim nyog, lossis lub kaw lus me dhau.
Tswv yim: PCR cov tshuaj tiv thaiv me me dhau lawm yuav ua rau qhov tseeb ntawm kev kuaj pom yuav txo qis.Nws yog qhov zoo tshaj plaws los siv cov tshuaj tiv thaiv uas tau pom zoo los ntawm cov cuab yeej PCR ntau kom rov ua dua qhov kev sim PCR tiag tiag.
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