EndoFree Maxi Plasmid Cov Khoom Siv (Spin Kem)
Kit Description:
Tag nrho cov txheej txheem rho tawm tsuas yog siv sijhawm 1 teev xwb, kom ntseeg tau tias kev ua haujlwm yooj yim thiab ceev.
Kev piav qhia
EndoFree Maxi Plasmid Cov Khoom Siv tau txais cov txheej txheem tshwj xeeb silica membrane adsorption kom ua tau zoo thiab tshwj xeeb khi plasmid DNA.Los ntawm kev sib koom ua ke tshwj xeeb Endotoxin Tshem Tawm Tsis Muaj P4 thiab EndoFree Maxi Lim Kab, endotoxin, cov protein thiab lwm yam impurities tuaj yeem raug tshem tawm zoo.Tag nrho cov txheej txheem rho tawm tsuas yog siv sijhawm 1 teev xwb, kom ntseeg tau tias kev ua haujlwm yooj yim thiab ceev.
Pom zoo cov kab mob ntau npaum li cas rau txhua qhov kev coj ua: Rau cov ntawv luam plasmid siab, nws raug nquahu kom siv 100 ml ntawm cov kab mob kab lis kev cai, thiab cov txiaj ntsig los ntawm qhov feem ntau yog nyob ib puag ncig 500-1500 μg.Rau cov plasmid qis, 200 ml kab mob kab lis kev cai xov xwm yog pom zoo kom tsim ib ncig ntawm 200-600 μg plasmids.
Cov khoom siv
| Khoom Cheebtsam | D909-E(10 kev npaj) |
| Buffer BL | 30 ml |
| Tsis P1 | 100 ml |
| Tsis P2 | 100 ml |
| Tsis P4 | 100 ml |
| Buffer DW2 | 44ml ib |
| Buffer IB | 30 ml |
| RNase A (10 mg/ml) | 1 ml ib |
| DNAPure Maxi Spin Kab | 10 pcs |
| FinePure Maxi Filter Syringe | 10 pcs |
| 50ml Collection Tubes | 10 pcs |
Kev kuaj pom ntawm Plasmid DNA Concentration thiab Purity
Lub purity thiab concentration ntawm cov extracted plasmid DNA tuaj yeem kuaj tau los ntawm agarose gel electrophoresis thiab ultraviolet spectrophotometer.Ib qho OD260 tus nqi ntawm 1 sib raug rau kwv yees li 50 μg / ml ntawm ob txoj hlua DNA.OD260 / OD280 tus nqi ntawm purified plasmid DNA feem ntau nyob rau hauv thaj tsam ntawm 1.7-1.9.Lub purified plasmid DNA tuaj yeem siv ncaj qha rau kev sim nrog cov kev xav tau siab huv, xws li kev hloov pauv ntawm tes lossis hauv vivo kev sim.
Daim ntawv thov Kit
Lub plasmid DNA muab rho tawm los ntawm cov khoom siv no tuaj yeem siv rau ntau yam haujlwm niaj hnub, suav nrog kev zom zaub mov, PCR, sib txuas, ligation, hloov pauv thiab hloov pauv rau ntau hom cell.
Cia thiab Txee lub neej
EndoFree Maxi Plasmid Cov Khoom Siv tuaj yeem khaws cia ntawm chav tsev kub (15-25 ° C) nyob rau hauv qhuav qhuav mus txog 12 lub hlis.Ntxiv RNase A rau Buffer P1 ua ntej siv thawj zaug, sib tov zoo thiab khaws cia ntawm 2-8 ° C.Qhov sib tov tuaj yeem khaws cia ruaj khov rau ntau tshaj 6 lub hlis.
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