Plant DNA Isolation Kit Genomic Plant DNA Purification Kits Reagents Protocol
Specifications
50 Preps, 100 Preps, 250 Preps
Cov khoom siv no siv cov kab DNA nkaus xwb uas tuaj yeem khi DNA, Foregene protease thiab cov kab ke tsis sib xws, uas ua kom yooj yim rau kev ua kom huv ntawm cov nroj tsuag genomic DNA.Cov genomic DNA zoo tuaj yeem tau txais tsis pub dhau 30 feeb, uas zam kev degradation ntawm genomic DNA.
Cov DNA-tsuas yog silica gel membrane siv nyob rau hauv kem spin yog Foregene cov khoom tshiab tshwj xeeb, uas tuaj yeem ua tau zoo thiab tshwj xeeb khi rau DNA, thiab ua kom tshem tawm RNA, impurity proteins, ions, polysaccharides, polyphenols thiab lwm yam organic compounds.
Cov khoom xyaw
| Buffer PL1, Tsis muaj PL2 |
| Buffer PW, Buffer WB, Buffer EB |
| Foregene Protease |
| DNA-Tsuas Kem |
| Cov lus qhia |
Nta & qhov zoo
■ Tsis muaj RNase cov kab mob: Cov DNA-Tsuas Kab uas muab los ntawm cov khoom siv ua rau nws tuaj yeem tshem RNA ntawm genomic DNA yam tsis muaj RNase ntxiv thaum lub sij hawm sim, tiv thaiv lub chaw soj nstuam los ntawm exogenous RNase.
■ Ceev ceev: Foregene Protease muaj kev ua haujlwm siab dua li cov proteases zoo sib xws thiab zom cov ntaub so ntswg sai dua.
■ Yooj yim: genomic DNA extraction ua haujlwm tuaj yeem ua tiav hauv 30 feeb.
■ Yooj yim: Lub centrifugation yog ua nyob rau hauv chav tsev kub, tsis muaj 4 ℃ low-temperature centrifugation los yog ethanol nag lossis daus ntawm DNA yuav tsum tau.
■ Kev nyab xeeb: tsis tas yuav muaj cov organic reagent.
■ Zoo: Cov purified genomic DNA muaj cov tawg loj, tsis muaj RNA, tsis muaj RNase, thiab cov ntsiab lus tsis tshua muaj ion, tuaj yeem ua tau raws li qhov yuav tsum tau muaj ntawm ntau yam kev sim.
Daim ntawv thov Kit
Haum rau kev rho tawm thiab ua kom huv ntawm genomic DNA los ntawm cov ntaub so ntswg tshiab lossis khov.
Kev khiav haujlwm
Daim duab
Cia thiab Txee lub neej
Cov khoom siv tuaj yeem khaws cia rau 12 lub hlis ntawm chav tsev kub (15-25 ℃) thiab 2-8 ℃ rau lub sijhawm ntev.
Foregene Protease Plus cov tshuaj muaj ib tug tshwj xeeb mis, uas yog active thaum khaws cia nyob rau hauv chav tsev kub rau lub sij hawm ntev (3 lub hlis);nws cov dej num thiab kev ruaj ntseg yuav zoo dua thaum khaws cia ntawm4 ℃, yog li nws pom zoo kom khaws cia ntawm 4 ℃, nco ntsoov tsis txhob khaws cia ntawm -20 ℃.
Phau ntawv qhia txog teeb meem
The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.
Tsawg tawm los lossis tsis muaj DNA
Feem ntau muaj ntau yam cuam tshuam rau cov txiaj ntsig ntawm genomic DNA, suav nrog qhov chaw ntawm tus qauv, hnub nyoog ntawm tus qauv, qhov chaw cia ntawm cov qauv, thiab kev ua haujlwm.
Genomic DNA tsis tuaj yeem tau txais thaum lub sijhawm rho tawm
1. Cov qauv ntawm cov ntaub so ntswg tsis raug cai los yog khaws cia ntev dhau lawm, ua rau cov DNA genomic degradation.
Kev pom zoo: Khaws cov ntaub so ntswg hauv cov kua nitrogen lossis -20°C;sim siv cov qauv sau tshiab rau kev rho tawm DNA genomic.
2. Cov qauv me me dhau lawm yuav ua rau cov DNA sib raug zoo tsis raug rho tawm.
Tswv yim: Rau cov qauv ntaub so ntswg uas tau muab khaws cia rau ntev los yog muaj cov genomic DNA degradation hnyav, tus nqi ntawm cov ntaub so ntswg tuaj yeem tsim nyog ntxiv txhawm rau txhawm rau rho tawm cov genomic DNA ntau heev.Tus nqi ntawm cov qauv tuaj yeem txiav txim siab raws li DNA xav tau, tab sis cov qauv tshiab yuav tsum tsis pub tshaj 100mg, thiab cov qauv qhuav yuav tsum tsis pub tshaj 30mg.
3. Cov qauv tsis yog hauv av nrog kua nitrogen los yog muab tso rau ntev heev tom qab cov kua nitrogen.
Cov lus qhia: Thaum lub sij hawm DNA rho tawm, cov qauv yuav tsum tau ua kom tiav hauv av nrog cov kua nitrogen kom tawg phab ntsa ntawm tes;Tom qab sib tsoo, thov hloov cov qauv hmoov rau PL1 preheated ntawm 65°C sai li sai tau (thaum cov hmoov av melted, cov genomic DNA yuav pib degrade sai).
4. Kev cia tsis raug ntawm Foregene Protease ua rau txo qis lossis tsis ua haujlwm.
Kev pom zoo: Txheeb xyuas qhov chaw cia ntawm Foregene Protease lossis hloov nws nrog Foregene Protease tshiab rau enzymatic hydrolysis.
5. Cov khoom siv tsis raug cai lossis khaws cia ntev dhau lawm, ua rau qee yam khoom siv tsis raug.
Daim ntawv pom zoo: Yuav cov khoom cog tshiab genomic DNA rho tawm cov khoom siv rau kev ua haujlwm.
6. Kev siv cov khoom siv tsis raug.
Cov Lus Qhia: Yuav Cov Khoom Siv Tsob Ntoo DNA Tso Cai rau cov qauv rau kev rho tawm thiab ntxuav cov nroj tsuag genomic DNA.
7. Buffer WB yam tsis tau ntxiv atsis muaj dej ethanol.
Kev pom zoo: Nco ntsoov ntxiv qhov tseeb ntim ntawm ethanol rau Buffer WB.
8. Lub eluent tsis yog dripped mus rau silica membrane kom raug.
Tswv yim: Ntxiv pre-heated eluent ntawm 65℃dropwise mus rau nruab nrab ntawm cov silica gel daim nyias nyias, thiab cia nws nyob rau hauv chav tsev kub rau 5 feeb los ua kom cov elution efficiency.
Kev rho tawm kom tau txais cov txiaj ntsig qis genomic DNA
1. Cov qauv no raug muab khaws cia tsis raug lossis khaws cia ntev dhau lawm, ua rau muaj qhov degradation ntawm genomic DNA.
Kev pom zoo: Khaws cov ntaub so ntswg ntawm -20℃;sim siv cov ntaub so ntswg tshiab tshiab rau kev rho tawm DNA genomic.
2. Yog tias cov qauv ntawm cov ntaub so ntswg me me dhau, cov genomic DNA rho tawm yuav tsawg dua.
Cov lus qhia: Qee cov qauv cog muaj cov dej nplua nuj, xws li cov nroj tsuag dej xws li algae, thiab lwm yam., qhov ntau npaum li cas tuaj yeem tsim nyog los yog cov dej tuaj yeem dehydrated me ntsis ua ntej ua haujlwm.
3. Cov qauv no tsis huv hauv av nrog cov kua nitrogen los yog raug tso rau hauv chav sov ntev ntev tom qab sib tsoo.
Tswv yim: Cov kua nitrogen sib tsoo yuav tsum txaus, thiab cov qauv ntawm cov phab ntsa yuav tsum tau tawg kom ntau li ntau tau;tam sim ntawd tom qab sib tsoo, cov qauv hmoov yuav tsum raug xa mus rau 65℃preheated Buffer PL1 rau cov kauj ruam tom ntej.
4. Tsis siv cov khoom siv kom raug.
Daim ntawv pom zoo: Siv cov khoom siv cog DNA cais tawm rau kev rho tawm thiab ntxuav cov nroj tsuag genomic DNA.
5. Kev cia tsis raug ntawm Foregene Protease ua rau txo qis lossis tsis ua haujlwm.
Kev pom zoo: Txheeb xyuas qhov chaw cia ntawm Foregene Protease lossis hloov nws nrog Foregene Protease tshiab rau enzymatic hydrolysis.
6. Eluent teeb meem
Kev pom zoo: Thov siv Buffer EB rau elution;yog siv ddH2O los yog lwm yam eluents, xyuas kom meej pH ntawm eluent nyob nruab nrab ntawm 7.0-8.5.
7. Lub eluent tsis yog dripped kom raug
Tswv yim: Thov ntxiv cov elution nco mus rau nruab nrab ntawm cov silica membrane thiab cia nws nyob rau hauv chav tsev kub li 5 feeb kom nce elution efficiency.
8. Lub ntim eluent tsawg dhau
Tswv yim: Thov siv qhov eluent rau genomic DNA elution raws li cov lus qhia, yam tsawg kawg nkaus tsis pub tsawg tshaj 100ua m l.
Extracted genomic DNA nrog tsawg purity
Qhov qis purity ntawm genomic DNA yuav ua rau tsis ua hauj lwm los yog cov nyhuv tsis zoo ntawm cov kev sim qis qis, xws li: cov enzyme tsis tuaj yeem txiav, thiab lub hom phiaj gene fragment tsis tuaj yeem tau los ntawm PCR.
1. Lwm yam kab mob sib kis, RNA paug.
Kev tshuaj xyuas: Tsis siv neeg PW tsis siv los ntxuav kab;Tsis PW tsis tau siv los ntxuav lub kem ntawm qhov tseeb centrifugation ceev.
Tswv yim: sim xyuas kom meej tias tsis muaj nag lossis daus nyob rau hauv lub supernatant thaum lub supernatant dhau los ntawm kab;Nco ntsoov ntxuav lub purification kem nrog Buffer PW raws li cov lus qhia, thiab cov kauj ruam no tsis tuaj yeem raug tshem tawm.
2. impurity ion muaj kuab paug.
Kev tshuaj xyuas: Buffer WB ntxuav kem raug tshem tawm lossis tsuas yog ntxuav ib zaug xwb, ua rau muaj cov kab mob ionic seem.
Kev pom zoo: Nco ntsoov ntxuav ob zaug nrog Buffer WB raws li cov lus qhia kom tshem tawm cov ions seem ntau li ntau tau.
3. Cov kab mob RNase.
Kev tsom xam: Exogenous RNase yog ntxiv rau qhov tsis;Kev ntxuav tsis raug hauv Buffer PW yuav ua rau RNase seem thiab cuam tshuam rau cov kev sim RNA hauv qab, xws li hauv vitro transcription.
Cov lus qhia: Foregene series nucleic acid extraction kits tuaj yeem tshem tawm RNA yam tsis muaj RNase ntxiv, thiab tag nrho cov reagents hauv Plant DNA Isolation Kit tsis xav tau RNase;Nco ntsoov ntxuav lub purification kem nrog Buffer PW raws li cov lus qhia, thiab cov kauj ruam no tsis tuaj yeem raug tshem tawm.
4. Ethanol residues.
Kev tshuaj xyuas: Tom qab ntxuav lub purification kem nrog Buffer WB, tsis muaj lub raj centrifugation tau ua.
Kev pom zoo: Ua raws li cov lus qhia kom tsim nyog khoob raj centrifugation.
Phau ntawv qhia:
Plant DNA Isolation Kit Instruction Manual
