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Tsiaj Daim ntaub ncaj qha PCR cov khoom siv
Cov khoom siv no siv cov cim lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm cov tsiaj cov nqaij mos rau PCR kev cuam tshuam, yog li nws tshwj xeeb tshaj yog rau kev ntsuas caj ces loj.
Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65°C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2× PCR Yooj YimTM Sib tov muaj qhov muaj peev xwm tiv taus zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj los kuaj raws li tus qauv tsim rau kev ua kom tau zoo thiab meej. Qhov kev lees paub no muaj cov Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis, PCR optimizer thiab stabilizer.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
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Mouse Tiav SuperDirect PCR Khoom
Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm nas tw thiab nas lub pob ntseg kuaj rau PCR cov tshuaj tiv thaiv, yog li nws tshwj xeeb rau kev kuaj caj ces loj.
Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm chav sov (20-25 ° C)Cov. Tsis muaj cua sov yuav tsum muaj, thiab lwm yam txheej txheem xws li cov protein thiab RNA tshem tawm tsis tas. Cov nqi tso tawm ntawm DNA tuaj yeem siv los ua Tus Qauv rau PCR cov tshuaj tiv thaiv.
2 × M-PCR EasyTM Mix muaj kev tiv thaiv zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj los sim ua qhov qauv piv txwv rau kom ua haujlwm tau zoo thiab meej. Qhov reagent no muaj Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis cuam tshuam, PCR optimizer thiab stabilizer.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
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Tsiaj Daim Ntaub Ncaj Ncaj Ncaj Ncaj Tes Khoom PCR-UNG
Cov khoom siv no siv cov cim lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm cov tsiaj cov nqaij mos rau PCR kev cuam tshuam, yog li nws tshwj xeeb tshaj yog rau kev ntsuas caj ces loj.
Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65°C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2×PCR Yooj YimTM Sib tov (UNG) siv dUTP hloov ntawm dTTP raws li 2×PCR Yooj YimTM Sib tov, thiab ntxiv rau UNG enzyme (Uracil-N-glycosylase) uas tuaj yeem thuam cov qauv uas muaj dUTP tib lub sijhawm. Ua ntej PCR cov tshuaj tiv thaiv, UNG enzyme yog siv los ua kom tsis zoo rau PCR cov khoom uas muaj cov uracil. Qhov UNG enzyme yuav tsis muaj dab tsi cuam tshuam rau tus qauv uas tsis muaj uracil, yog li ua kom ntseeg tau qhov tshwj xeeb thiab qhov tseeb ntawm qhov ua kom nrov dua thiab tiv thaiv kev muaj peev xwm ntawm PCR cov khoom sib kis thaum ntsuas kev tshuaj ntsuam loj.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
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Cov nas twv ncaj qha PCR cov khoom siv
Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA ntawm nas tw, pob ntseg, cov leeg thiab lwm cov nqaij ua rau PCR cov tshuaj tiv thaiv, yog li nws tshwj xeeb rau kev ntsuas caj ces loj.
Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65 ° C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2 × M1-PCR EasyTM Mix muaj kev tiv thaiv zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj mus kuaj raws li tus qauv tsim kom tau txais txiaj ntsig thiab tshwj xeeb. Qhov reagent no muaj Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis cuam tshuam, PCR optimizer thiab stabilizer.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
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Nas Tail Ncaj PCR Cov Khoom-UNG
Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA ntawm nas tw, pob ntseg, cov leeg thiab lwm cov nqaij ua rau PCR cov tshuaj tiv thaiv, yog li nws tshwj xeeb rau kev ntsuas caj ces loj.
Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65 ° C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2 × M1-PCR EasyTM Mix (UNG) siv dUTP hloov ntawm dTTP raws li 2 × M1-PCR EasyTM Mix (UNG), thiab ntxiv UNG enzyme (Uracil-N-glycosylase) uas tuaj yeem thuam cov qauv uas muaj lub dUTP ntawm tib lub sij hawm. Ua ntej PCR cov tshuaj tiv thaiv, UNG enzyme yog siv los ua kom tsis zoo rau PCR cov khoom uas muaj cov uracil. Qhov UNG enzyme yuav tsis muaj kev cuam tshuam rau tus qauv uas tsis muaj uracil, yog li ua kom ntseeg tau qhov tshwj xeeb thiab qhov tseeb ntawm amplification thiab tiv thaiv kev muaj peev xwm ntawm kev sim tshuaj ntsuam loj. Muaj paug khoom ntawm PCR tau tshwm sim.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
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Zebra Ntses Ntsig PCR Khoom Siv
Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm zebrafish thiab lwm cov ntses dej ntses tshiab, ntses fins lossis cov qe ntses coj mus kuaj rau PCR cov tshuaj tiv thaiv, yog li nws tsim tshwj xeeb rau kev kuaj caj ces loj. Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65 ° C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2 × PCR EasyTM Mix muaj kev tiv thaiv zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj mus kuaj raws li tus qauv tsim kom tau txais txiaj ntsig thiab tshwj xeeb. Qhov reagent no muaj ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis haum, PCR optimizer thiab stabilizer. Siv ua ke nrog cov lysis tsis tuaj yeem ua kom sai thiab yooj yim kuaj cov qauv, thiab muaj cov yam ntxwv ntawm kev rhiab siab, muaj zog tshwj xeeb, thiab muaj kev ruaj khov.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
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Zebra Ntses Ncaj PCR Khoom-UNG
Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm zebrafish thiab lwm cov ntses dej ntses tshiab, ntses fins lossis cov qe ntses coj mus kuaj rau PCR cov tshuaj tiv thaiv, yog li nws tsim tshwj xeeb rau kev kuaj caj ces loj. Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65 ° C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2 × PCR EasyTM Mix (UNG) siv dUTP hloov ntawm dTTP raws li 2 × PCR EasyTM Mix, thiab ntxiv rau UNG enzyme (Uracil-N-glycosylase) uas tuaj yeem thuam cov qauv uas muaj dUTP tib lub sijhawm. Ua ntej PCR cov tshuaj tiv thaiv, UNG enzyme yog siv los ua kom tsis zoo rau PCR cov khoom uas muaj cov uracil. Qhov UNG enzyme yuav tsis muaj kev cuam tshuam rau tus qauv uas tsis muaj uracil, yog li ua kom ntseeg tau qhov tshwj xeeb thiab qhov tseeb ntawm amplification thiab tiv thaiv kev muaj peev xwm ntawm kev sim tshuaj ntsuam loj. Muaj paug khoom ntawm PCR tau tshwm sim.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
