Mouse Tiav SuperDirect PCR Khoom

Mouse Tiav SuperDirect PCR Khoom

Cov Khoom Siv Qhia:

Cov khoom siv no siv cov lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm nas tw thiab nas lub pob ntseg kuaj rau PCR cov tshuaj tiv thaiv, yog li nws tshwj xeeb rau kev kuaj caj ces loj.

Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm chav sov (20-25 ° C)Cov. Tsis muaj cua sov yuav tsum muaj, thiab lwm yam txheej txheem xws li cov protein thiab RNA tshem tawm tsis tas. Cov nqi tso tawm ntawm DNA tuaj yeem siv los ua Tus Qauv rau PCR cov tshuaj tiv thaiv.

2 × M-PCR EasyTM Mix muaj kev tiv thaiv zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj los sim ua qhov qauv piv txwv rau kom ua haujlwm tau zoo thiab meej. Qhov reagent no muaj Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis cuam tshuam, PCR optimizer thiab stabilizer.

D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.


Khoom Nthuav dav

Cov khoom lag luam

Cov Lus Qhia Tshwj Xeeb

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Cov khoom siv Cheebtsam

Nqe I

Tsis NP

Foregene Protease Ntxiv rau Kuv

6 × DNA Chaw Tsim Khoom Tsis Muaj

Ntu II

2 × M PCR EasyTM Sib tov

Cov Lus Qhia

Nta & qhov zoo

■ Tsis muaj sijhawm siv thiab kim DNA ua kom huv yuav tsum muaj.

Cov qauv xav tau yog me me, tsuas yog nqa cov pob tw nas (los yog lub pob ntseg nas nrog lub taub ntawm 2-5 hli) siab tshaj 2mm.

■ Yooj yim khiav lag luam, nws tuaj yeem lysed tsis muaj cua sov kom tsis ua rau lub zog, thiab cov khoom lysed tuaj yeem cuam tshuam ncaj qha rau PCR cov tshuaj tiv thaiv.

■ Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) tuaj yeem siv tau los txiav cov DNA ntawm cov noob caj noob ntoo hauv cov txheej txheem rub tawm.

■ Qhov zoo ntawm PCR ua rau PCR muaj qhov ntau dua qhov siab dua thiab qhov muaj peev xwm siab dua rau PCR cov tshuaj tiv thaiv kev tiv thaiv.

Khoom siv tsis

Daim Ntawv Thov: Nas tus tw, nas pob ntseg, cov leeg thiab lwm cov ntaub so ntswg.

DNA tso tawm los ntawm cov qauv lysis: tsuas yog siv ua PCR qauv.

Cov khoom siv tuaj yeem siv rau cov laj thawj nram qab no: kev txheeb xyuas tus mob transgenosis, kev tua tsiaj ntawm tsiaj, thiab lwm yam.

Ua haujlwm ntws

wk-flw-mouse-tail-SUPER-direct-PCR

Cia thiab txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum ceev cia thaum 2-8 ℃.

Lub reagent Buffer FP tuaj yeem khaws cia rau 12 lub hlis hauv kev mob qhuav; nws tuaj yeem khaws cia ntawm 2-8 ℃ rau khaws cia ntev dua.

Reagent Foregene Protease muaj cov qauv tsis sib xws. Txhawm rau xyuas kom nws cov haujlwm thiab ruaj khov, thov khaws nws ntawm 4 ℃ rau 12 lub hlis.

Reagent 6 × DNA Chaw thau khoom tsis tuaj yeem khaws cia ntawm 4 ℃ lossis -20 ℃ rau lub sijhawm ntev.

Ntu II ntawm cov khoom siv no yuav tsum ceev cia ntawm -20 ℃.

Reagent 2 × PCR EasyTM Mix, yog tias siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ℃ rau lub sijhawm cia ntev (siv ntev li 10 hnub).


  • Yav dhau los:
  • Tom ntej:

  • Sau koj cov lus ntawm no thiab xa rau peb