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Cov Khoom Siv Cell Ncauj RT qPCR
Cov khoom siv no siv cov cim lysis tsis zoo uas tuaj yeem tso sai sai RNA los ntawm kev kuaj kab mob ntawm tes rau RT-qPCR kev cuam tshuam, yog li tshem tawm lub sijhawm ua haujlwm thiab kev siv zog ua haujlwm RNA kev ua kom huv. RNA template tuaj yeem tau txais hauv 7 feeb xwb. Lub 5 × Ncaj Qha RT Mix thiab 2 × Ncaj QPCR Kev Sib Tw-SYBR reagents muab los ntawm cov khoom siv tau sai thiab muaj peev xwm tau txais cov txiaj ntsig tiag tiag ntawm lub sijhawm PCR.
5 RT Direct RT Mix thiab 2 × Ncaj QPCR Mix-SYBR muaj cov tshuaj tiv thaiv zoo, thiab lysate ntawm cov qauv tuaj yeem siv los ua tus qauv rau RT-qPCR ncaj qha. Cov khoom siv no muaj qhov tshwj xeeb RNA siab-kev sib raug zoo Foregene thim rov qab, thiab Kub D-Taq DNA polymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis haum, PCR optimizer thiab stabilizer.
