Tuam Tshoj Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Ntsuas Tail) (rau Genotyping) Chaw tsim tshuaj paus thiab tsum |Foregene

Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Ntaus Tail) (rau Genotyping)

Kit Description:

Ncaj nraim siv nas tail lysate ua tus qauv rau PCR amplification, tsis muaj cais rho tawm ntawm DNA, ceev thiab siab rhiab heev.

◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.

◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.

◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.

◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.

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Product Detail

Khoom cim npe

Cov lus piav qhia

Cov khoom siv no siv cov txheej txheem tshwj xeeb lysis tsis tuaj yeem tso tawm genomic DNA sai sai los ntawm nas tails, pob ntseg, cov leeg thiab lwm yam ntaub so ntswg kuaj rau PCR cov tshuaj tiv thaiv, yog li nws yog qhov tshwj xeeb tshaj yog rau kev kuaj kab mob loj.

Cov txheej txheem ntawm kev tso genomic DNA los ntawm lysis tsis yog ua tiav hauv 10-30 feeb ntawm 65 ° C.Tsis muaj lwm yam txheej txheem xws li protein thiab RNA tshem tawm, thiab cov kab DNA tso tawm tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.

2 × M1-PCR Ib qho yooj yim^TMMix muaj zog kam rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv, thiab tuaj yeem siv cov lysate ntawm cov qauv los kuaj ua tus qauv rau kev ua kom muaj txiaj ntsig thiab tshwj xeeb.Cov reagent no muaj Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, cov tshuaj tiv thaiv tsis zoo, PCR optimizer thiab stabilizer.

D-Taq DNA polymerase yog DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau cov tshuaj PCR ncaj qha.D-Taq DNA polymerase muaj kev ua siab ntev rau ntau yam PCR cov tshuaj tiv thaiv, thiab tuaj yeem ua kom muaj txiaj ntsig zoo ntawm DNA hauv ntau cov tshuaj tiv thaiv nyuaj, thiab qhov kev nthuav dav tuaj yeem ncav cuag 2Kb / min, uas yog tshwj xeeb tshaj yog haum rau Direct PCR cov tshuaj tiv thaiv.

Specifications

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Cov khoom siv

Ntu I	Buffer MP
	Foregene Protease Plus
	6 × DNA Loading Buffer
Ntu II	2 × M1 PCR Ib qho yooj yim^TMSib tov
Cov lus qhia

Nta & qhov zoo

■Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.

■ Cov qauv xav tau me me, tsawg li 5mg nas cov ntaub so ntswg lossis 2-5mm nas tus Tsov tus tw tuaj yeem kuaj tau.

■ Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab kev sib tsoo, thiab kev ua haujlwm yooj yim.

■ Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv.

Cov khoom siv parameter

Daim ntawv thov: nas Tail, nas pob ntseg, cov leeg nqaij thiab lwm yam ntaub so ntswg.

DNA tso tawm los ntawm cov qauv lysis: tsuas yog siv los ua PCR template.

Cov khoom siv tuaj yeem siv rau lub hom phiaj hauv qab no: kev txheeb xyuas ntawm transgenosis, tsiaj genotyping, thiab lwm yam.

Kev khiav haujlwm

Cia thiab Txee lub neej

Ntu I ntawm cov khoom siv no yuav tsum muab khaws cia ntawm 2-8℃.

Lub reagent Buffer AL tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav;nws tuaj yeem khaws cia ntawm 2-8℃rau ntev cia.

Reagent Foregene Protease muaj cov qauv tshwj xeeb.Txhawm rau kom ntseeg tau tias nws cov haujlwm thiab kev ruaj ntseg, thov khaws cia ntawm 4℃rau 12 lub hlis.

Reagent 6×DNA Loading Buffer tuaj yeem khaws cia ntawm 4℃los yog -20℃ntev.

Ntu II ntawm cov khoom siv no yuav tsum muab khaws cia ntawm -20℃.

Reagent 2×M1 PCR yooj yim^TMSib tov, yog siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4℃rau lub sij hawm luv luv (siv li ntawm 10 hnub).

Yav dhau los: Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent (Ntaus Tail) (rau Genotyping)

Tom ntej: Tsiaj Tissue Direct PCR cov khoom siv (tsis siv cov cuab yeej Sampling) raws tu qauv

Sau koj cov lus ntawm no thiab xa tuaj rau peb