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Lnc-RT Hero ᵀᴹ I (Nrog gDNase) (Super Premix rau thawj-strand cDNA hluavtaws los ntawm lncRNA)
Lnc-RT Tus Poj NiamTM Kuv (Nrog gDNase) yog ib qho kev hloov pauv ntawm kev hloov pauv tshwj xeeb tsim rau lncRNA kom sai tshem tawm cov kab mob DNA sib kis. 5 × gDNase Mix tuaj yeem tshem tawm cov genome ntxiv hauv RNA ntawm 42 ° C rau 2 feeb, zoo tiv thaiv kev cuam tshuam ntawm genome ntawm qPCR cov txiaj ntsig.
5 × L-RT Tus HeroTM Mix muaj Foregene LncRNA Rov Qab Transcriptase tshwj xeeb uas tsim los ntawm Foregene, uas yog hom tshiab ntawm cov ntaub ntawv xa rov qab tshwj xeeb rau lncRNA thiab lwm qhov ntev ntev RNA complex templates, nrog muaj zog RNA affinity thiab ntau dua reversal cov ntaub ntawv sau txuag. Lub kaw lus zoo ua rau kev hloov pauv hloov nrawm nrawm dua thiab tuaj yeem hloov tau yooj yim RNA cov qauv muaj cov ntsiab lus GC siab thiab cov qauv txheej txheem theem nrab. Thawj cov strand cDNA synthesis tuaj yeem ua tiav ntawm 42 ° C hauv 15 feeb.
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Lub Sij Hawm Tiag PCR Easyᵀᴹ-Taqman
Qhov 2X Tiag PCR Yooj YimTM Mix-Taqman muab los ntawm Lub Sij Hawm PCR Yooj YimTM-Taqman cov khoom siv yog qhov kev tsim kho tshiab premix uas siv tshwj xeeb fluorescent kuaj pom tseeb rau Lub Sij Hawm PCR ua kom nrov nrov, tuaj yeem txhim kho cov khoom lag luam tshwj xeeb thiab cov tshuaj tiv thaiv rhiab. ROX muab raws li kev tswj xim sab hauv.
2X Tiag PCR Yooj YimTM Mix-Taqman muaj Foregene lub cim kub-pib Taq DNA Polymerase. Piv nrog rau kev ua kom zoo li lub cev Taq, nws muaj qhov zoo ntawm kev ua kom nrov loj, muaj zog muaj peev xwm nthuav dav thiab tsis tshua muaj qhov tsis tseem ceeb. Nws tuaj yeem txo qhov tsis meej tshwj xeeb thiab txhim kho qhov tseeb ntawm PCR.
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Lub Sij Hawm Tiag PCR Yooj Yooj Yim BR-SYBR Cov I ntsuab
Qhov 2X Tiag PCR Yooj YimTM Mix-SYBR muab los ntawm Lub Sijhawm PCR Yooj Yooj YimTM-SYBR ntsuab Kuv cov khoom siv tshiab yog qhov khoom siv tshiab premix uas siv SYBR Green I rau Lub Sijhawm PCR ua kom nrov dua, uas tuaj yeem txhim kho tau cov khoom lag luam tshwj xeeb thiab cov tshuaj tiv thaiv. ROX muab raws li kev tswj xim sab hauv. Lub fluorescence siv ntawm cov khoom siv no yog 3-5 zaug ntawm cov khoom uas zoo sib xws, uas tuaj yeem xav paub ntau dua thiab piav qhia meej txog qhov kev nkag siab ntawm lub hom phiaj qauv DNA.
2X Tiag PCR Yooj YimTM Mix-SYBR muaj Foregene lub cim kub-pib Taq DNA Polymerase. Piv nrog rau kev ua kom zoo li lub cev Taq, nws muaj qhov zoo ntawm kev ua kom nrov loj, muaj zog muaj peev xwm nthuav dav thiab tsis tshua muaj qhov tsis tseem ceeb. Nws tuaj yeem txo qhov tsis meej tshwj xeeb thiab txhim kho qhov tseeb ntawm PCR.
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PCR Heroᵀᴹ (Nrog Dye)
Qhov 2X Tiag PCR Yooj YimTM Mix-SYBR muab los ntawm Lub Sijhawm PCR Yooj Yooj YimTM-SYBR ntsuab Kuv cov khoom siv tshiab yog qhov khoom siv tshiab premix uas siv SYBR Green I rau Lub Sijhawm PCR ua kom nrov dua, uas tuaj yeem txhim kho tau cov khoom lag luam tshwj xeeb thiab cov tshuaj tiv thaiv. ROX muab raws li kev tswj xim sab hauv. Lub fluorescence siv ntawm cov khoom siv no yog 3-5 zaug ntawm cov khoom uas zoo sib xws, uas tuaj yeem xav paub ntau dua thiab piav qhia meej txog qhov kev nkag siab ntawm lub hom phiaj qauv DNA.
2X Tiag PCR Yooj YimTM Mix-SYBR muaj Foregene lub cim kub-pib Taq DNA Polymerase. Piv nrog rau kev ua kom zoo li lub cev Taq, nws muaj qhov zoo ntawm kev ua kom nrov loj, muaj zog muaj peev xwm nthuav dav thiab tsis tshua muaj qhov tsis tseem ceeb. Nws tuaj yeem txo qhov tsis meej tshwj xeeb thiab txhim kho qhov tseeb ntawm PCR.
