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Dab tsi yog qhov txawv ntawm PCR, RT-PCR, qPCR, thiab RT-qPCR
PCR yog ib txoj hauv kev siv los nthuav DNA los ntawm ib qho me me ntawm DNA qauv.RT-PCR siv cov ntawv thim rov qab los tsim cov qauv DNA los ntawm RNA qhov chaw uas tuaj yeem nthuav dav.PCR thiab RT-PCR feem ntau yog qhov kawg qhov tshwm sim, thaum qPCR thiab RT-qPCR siv cov kinetics ntawm tus nqi ntawm cov khoom synt ...Nyeem ntxiv -
Lub Forefront ntawm Tshuaj Tshawb Nrhiav: Targeting RNA nrog me me molecules
Pfizer's mRNA tshuaj tiv thaiv rau COVID tau ua rau muaj kev mob siab rau kev siv ribonucleic acid (RNA) ua lub hom phiaj kho.Txawm li cas los xij, tsom RNA nrog cov molecules me yog qhov nyuaj heev.RNA tsuas muaj plaub lub tsev thaiv: adenine (A), cytosine (C), guanine (G), thiab uracil (U) uas hloov thymin ...Nyeem ntxiv -
Cov tshuaj nucleic acid yog dab tsi?
"Nucleic acid tshuaj" siv "nucleic acid," uas xa mus rau cov khoom xws li DNA thiab RNA uas tswj cov ntaub ntawv caj ces, ua tshuaj.Cov no tso cai rau lub hom phiaj ntawm cov molecules xws li mRNA thiab miRNA uas tsis tuaj yeem raug tsom nrog cov tshuaj qis molecular hnyav thiab tshuaj tiv thaiv kab mob, thiab muaj g ...Nyeem ntxiv -
Qee qhov kev paub txog siRNA hauv RT-PCR
1. Kev paub yooj yim (yog tias koj xav pom qhov kev sim, thov xa ncaj qha mus rau ntu thib ob) Raws li cov lus pom zoo ntawm cov pa PCR, Lub sijhawm tiag tiag PCR feem ntau saib xyuas qhov kev hloov pauv ntawm cov khoom lag luam amplification hauv txhua lub voj voog ntawm PCR amplification. cov tshuaj tiv thaiv hauv lub sijhawm tiag tiag thr...Nyeem ntxiv -
RT-PCR kev sim tshuaj tiv thaiv kab ke optimization txoj kev qhia ntxaws ntxaws
Ib 、 Ua kom muaj kev nkag siab zoo ntawm cov tshuaj tiv thaiv: 1. cais cov RNA zoo: Ua tiav cDNA synthesis los ntawm cov khoom zoo RNA.Qhov zoo RNA yuav tsum tsawg kawg yog tag nrho-ntev thiab tsis pub rov qab transcriptase inhibitors xws li EDTA lossis SDS.Qhov zoo ntawm RNA txiav txim siab qhov siab tshaj plaws ...Nyeem ntxiv -
Ua kom tiav PCR primer tsim thiab PCR cov ntsiab lus
Primer tsim hauv paus (99% cov teeb meem tuaj yeem daws tau) 1. Primer ntev: Phau ntawv yuav tsum muaj 15-30bp, feem ntau yog li 20bp.Qhov tseeb yog qhov zoo dua yog 18-24bp los xyuas kom meej qhov tshwj xeeb, tab sis ntev dua qhov zoo dua, ntev primer kuj yuav txo tau qhov tshwj xeeb, thiab txo qhov tawm los.2. Pem...Nyeem ntxiv -
Kev ceev faj rau qRT-PCR muab tso ua ke los ntawm tus kws kho mob laus mob siab rau
Txhua leej txhua tus tab tom tham txog lub hauv paus ntsiab lus ntawm qRT-PCR kev sim, kev tsim qauv, kev txhais lus, thiab lwm yam, tab sis kuv xav tias kuv yuav tsum qhia rau koj txog kev sim ua haujlwm ntawm qRT-PCR.Nws yog me me, tab sis nws yog hais txog cov txiaj ntsig.Ua ntej ua qRT-PCR, peb yuav tsum muaj kev nkag siab meej ntawm peb ...Nyeem ntxiv -
Kev ceev faj rau RT-qPCR
RT-qPCR kev sim suav nrog RNA rho tawm thiab kev soj ntsuam zoo, rov qab sau thiab qPCR peb kauj ruam, txhua kauj ruam muaj kev ceev faj ntau, peb yuav qhia kom meej hauv qab no.Ⅰ.Kev soj ntsuam zoo RNA Hauv RT-qPCR kev sim, tom qab ua tiav ntawm RNA rho tawm, ...Nyeem ntxiv -
Cov lus qhia ntxaws txog PCR lub sijhawm
1. Kev nkag siab thaum pib ntawm theem no, peb yuav tsum nkag siab qee lub ntsiab lus thiab cov ntsiab lus, kom tsis txhob ua yuam kev ntawm peb cov neeg laus, xws li: Q: Qhov txawv ntawm RT-PCR, qPCR, Real-time PCR, yog dab tsi? thiab real-time RT-PCR?Teb: RT-PCR yog rov qab transcription PCR (rov qab t ...Nyeem ntxiv -
PCR, ntau PCR, Hauv qhov chaw PCR, Rov qab PCR, RT-PCR, qPCR (1) - PCR
PCR, ntau PCR, Hauv qhov chaw PCR, Rov qab PCR, RT-PCR, qPCR (1)– PCR Peb yuav txheeb xyuas cov ntsiab lus, cov kauj ruam thiab cov ntsiab lus ntawm ntau yam PCR Ⅰ.PCR Polymerase Chain Reaction, hu ua PCR, yog lub tshuab hluav taws xob molecular uas siv los nthuav cov DNA tshwj xeeb.Nws tuaj yeem suav tias yog ib qho ...Nyeem ntxiv -
qRT-PCR txoj cai kev sim
RT-qPCR yog tsim los ntawm cov tshuab PCR zoo tib yam.Nws ntxiv cov tshuaj fluorescent (fluorescent dyes lossis fluorescent probes) rau cov txheej txheem PCR cov tshuaj tiv thaiv ib txwm muaj, thiab kuaj pom cov txheej txheem PCR annealing thiab txuas ntxiv nyob rau lub sijhawm raws li lawv qhov sib txawv luminescent mechanisms.Fluorescent kos npe...Nyeem ntxiv -
RT-PCR kev sim tshuaj tiv thaiv kab ke optimization txoj kev qhia ntxaws ntxaws
Ⅰ.Ua kom muaj qhov rhiab heev ntawm cov tshuaj tiv thaiv kab mob: 1. Sib cais siab-zoo RNA: Ua tiav cDNA synthesis los ntawm high-quality RNA.High-quality RNA yuav tsum xyuas kom tsawg kawg nkaus ntev dua thiab tsis muaj cov inhibitors uas tsis muaj cov enzymes kaw, xws li EDTA lossis SDS.Qhov zoo...Nyeem ntxiv
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