Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent (zebrafish)
Kit Description:
Ncaj nraim siv zebrafish lysate ua tus qauv rau PCR amplification, tsis muaj cais rho tawm ntawm DNA, ceev thiab siab rhiab heev.
◮Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
◮Cov qauv xav tau me me, tsuas yog siv ib lub noob xwb.
◮Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab crushing, thiab kev ua haujlwm yog yooj yim.
◮Qhov kev ua kom zoo PCR ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv.
Cov lus piav qhia
Cov khoom no siv cov txheej txheem tshwj xeeb lysis tsis tuaj yeem tso tawm cov genomic DNA los ntawm zebrafish thiab lwm cov ntaub so ntswg ntawm cov ntses dej tshiab, cov fins lossis cov qe ntses kuaj rau PCR cov tshuaj tiv thaiv, yog li nws yog qhov tshwj xeeb tshaj yog tsim rau kev kuaj kab mob loj.Cov txheej txheem ntawm kev tso genomic DNA los ntawm lysis tsis yog ua tiav hauv 10-30 feeb ntawm 65 ° C.Tsis muaj lwm yam txheej txheem xws li protein thiab RNA tshem tawm, thiab cov kab DNA tso tawm tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2 × PCR Ib qho yooj yimTMMix muaj zog kam rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv, thiab tuaj yeem siv cov lysate ntawm cov qauv los kuaj ua tus qauv rau kev ua kom muaj txiaj ntsig thiab tshwj xeeb.Cov reagent no muaj ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis zoo, PCR optimizer thiab stabilizer.Siv ua ke nrog cov lysis tsis tuaj yeem tshawb xyuas cov qauv sai thiab yooj yim, thiab muaj cov yam ntxwv ntawm kev kub siab, muaj zog tshwj xeeb, thiab kev ruaj ntseg zoo.
D-Taq DNA polymerase yog DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau cov tshuaj PCR ncaj qha.D-Taq DNA polymerase muaj kev ua siab ntev rau ntau yam PCR cov tshuaj tiv thaiv, thiab tuaj yeem ua kom muaj txiaj ntsig zoo ntawm DNA hauv ntau cov tshuaj tiv thaiv nyuaj, thiab qhov kev nthuav dav tuaj yeem ncav cuag 2Kb / min, uas yog tshwj xeeb tshaj yog haum rau Direct PCR cov tshuaj tiv thaiv.
Specifications
50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns
Cov khoom siv
| Ntu I | Tsis pub FP |
| Foregene Protease | |
| 6 × DNA Loading Buffer | |
| Ntu II | 2 × PCR Ib qho yooj yimTMSib tov |
| Cov lus qhia | |
Nta & qhov zoo
■Tsis tas yuav siv sijhawm ntev thiab kim kho DNA purification.
■ Cov qauv xav tau me me, tsawg li 1mg ntses caudal fin lossis 10 qe ntses tuaj yeem kuaj tau.
■ Tsis muaj kev kho tshwj xeeb xws li kev sib tsoo thiab kev sib tsoo, thiab kev ua haujlwm yooj yim.
■ Optimized PCR system ua rau PCR muaj qhov tshwj xeeb dua thiab muaj zog ua siab ntev rau PCR cov tshuaj tiv thaiv cov tshuaj tiv thaiv.
Cov khoom siv parameter
Daim ntawv thov: Zebra ntses thiab lwm yam ntses dej tshiab.
DNA tso tawm los ntawm cov qauv lysis: tsuas yog siv los ua PCR template.
Cov khoom siv tuaj yeem siv rau lub hom phiaj hauv qab no: kev txheeb xyuas ntawm transgenosis, tsiaj genotyping, thiab lwm yam.
Kev khiav haujlwm
Cia thiab Txee lub neej
Ntu I ntawm cov khoom siv no yuav tsum khaws cia ntawm 2-8 ℃.
Lub reagent Buffer FP tuaj yeem khaws cia rau 12 lub hlis nyob rau hauv qhuav qhuav;Nws tuaj yeem khaws cia ntawm 2-8 ℃ rau kev cia ntev dua.
v Reagent Foregene Protease muaj cov mis tshwj xeeb.Txhawm rau ua kom nws cov haujlwm thiab ruaj khov, thov khaws cia ntawm 4 ℃ rau 12 lub hlis.
vReagent 6 × DNA Loading Buffer tuaj yeem khaws cia ntawm 4 ℃ lossis -20 ℃ rau lub sijhawm ntev.
Ntu II ntawm cov khoom siv no yuav tsum khaws cia ntawm -20 ℃.
vReagent 2 × PCR EasyTM Mix, yog siv ntau zaus, nws tseem tuaj yeem khaws cia ntawm 4 ℃ rau lub sijhawm luv luv (siv li ntawm 10 hnub).
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