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Tsiaj Daim ntaub ncaj qha PCR cov khoom siv
Cov khoom siv no siv cov cim lysis tsis xws li tso tawm sai sai rau cov noob caj noob ces DNA los ntawm cov tsiaj cov nqaij mos rau PCR kev cuam tshuam, yog li nws tshwj xeeb tshaj yog rau kev ntsuas caj ces loj.
Cov txheej txheem ntawm kev tso cov genomic DNA los ntawm lysis buffer ua tiav tsis pub dhau 10-30 feeb ntawm 65°C. Tsis muaj lwm cov txheej txheem xws li cov protein thiab RNA tshem tawm yog qhov yuav tsum tau ua, thiab qhov tso tawm DNA tau tuaj yeem siv los ua tus qauv rau PCR cov tshuaj tiv thaiv.
2× PCR Yooj YimTM Sib tov muaj qhov muaj peev xwm tiv taus zoo rau PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem siv lysate ntawm cov qauv coj los kuaj raws li tus qauv tsim rau kev ua kom tau zoo thiab meej. Qhov kev lees paub no muaj cov Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, cov tshuaj tiv thaiv tsis, PCR optimizer thiab stabilizer.
D-Taq DNA polymerase yog cov DNA polymerase tshwj xeeb uas tsim los ntawm Foregene rau kev tiv thaiv PCR ncaj qha. D-Taq DNA polymerase muaj kev tiv thaiv zoo rau ntau yam ntawm PCR cov tshuaj tiv thaiv kev tiv thaiv, thiab tuaj yeem ua kom muaj nuj nqis ntau ntawm cov DNA hauv ntau cov txheej txheem kev tiv thaiv, thiab qhov ua kom nrov loj tuaj yeem ncav cuag 2Kb / min, uas tsim nyog tshwj xeeb rau cov tshuaj tiv thaiv PCR ncaj.
