Plant Total RNA Isolation Plus Kit
Kit Description:
Kev tshem tawm DNA zoo siv DNA-Tshuaj Kem
Tshem tawm DNA yam tsis tau ntxiv DNase
Yooj yim-tag nrho cov haujlwm ua tiav ntawm chav tsev kub
Fast-kev ua haujlwm tuaj yeem ua tiav hauv 30 feeb
Kev nyab xeeb - tsis muaj cov organic reagent siv
Specifications
50 Preps, 200 Preps
Cov khoom siv siv cov kab sib dhos thiab cov qauv tsim los ntawm Foregene, uas tuaj yeem tshem tawm cov purity siab thiab zoo tag nrho RNA los ntawm ntau cov ntaub so ntswg nrog cov polysaccharides siab lossis polyphenols cov ntsiab lus.Nws muab cov DNA-Tsev kem uas tuaj yeem tshem tawm genomic DNA los ntawm supernatant thiab cov ntaub so ntswg lysate.RNA nkaus xwb tuaj yeem khi RNA zoo.Cov khoom siv tuaj yeem ua cov qauv ntau ntawm tib lub sijhawm.
Tag nrho cov kab ke tsis muaj RNase, yog li RNA purified yuav tsis degraded.Buffer PRW1 thiab Buffer PRW2 tuaj yeem ua kom ntseeg tau tias RNA tau txais tsis muaj kab mob los ntawm cov protein, DNA, ions, thiab cov organic tebchaw.
Cov khoom siv
| Tsis PSL1, Tsis PS, Tsis PSL2 |
| Buffer PRW1, Buffer PRW2 |
| RNase-Dawb ddH2O, DNA-Tsav Kem |
| RNA-Tsuas Kem |
Nta & qhov zoo
■ Kev khiav hauj lwm ntawm chav tsev kub (15-25 ℃) thoob plaws hauv tag nrho cov txheej txheem, tsis muaj dej khov da dej thiab tsis kub centrifugation.
■ Ua kom tiav cov khoom siv RNase-Free, tsis tas yuav txhawj txog RNA degradation.
■ Tshwj xeeb tsim nyog rau kev ua kom huv ntawm RNA los ntawm cov qauv cog ntawm polysaccharides thiab polyphenols.
■ DNA-Cleaning Kem tshwj xeeb khi rau DNA, kom cov khoom siv tuaj yeem tshem tawm cov kab mob DNA sib kis yam tsis tau ntxiv DNase.
■ High RNA tawm los: RNA nkaus xwb Kem thiab cov mis tshwj xeeb tuaj yeem ua kom huv RNA zoo.
■ Ceev ceev: yooj yim rau kev khiav lag luam thiab tuaj yeem ua tiav hauv 30 feeb.
■ Kev nyab xeeb: tsis tas yuav muaj cov organic reagent.
■ Zoo: Cov purified RNA tawg yog cov purity siab, tsis muaj protein ntau thiab lwm yam impurities, thiab tuaj yeem ua tau raws li ntau yam kev sim hauv qab.
Khoom parameters
■ Cov ntawv thov hauv qab: thawj-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, thiab lwm yam.
■ Qauv: Cov ntaub so ntswg tshiab los yog khov cog ntawm polysaccharides thiab polyphenols
■ Siv: 50mg cov ntaub so ntswg
■ RNA binding peev xwm ntawm purification kem: 80 μg
■ Elution ntim: 50-200 μl
Daim ntawv thov khoom siv
Nws yog qhov tsim nyog rau kev rho tawm thiab ua kom huv ntawm tag nrho RNA los ntawm cov qauv tshiab los yog khov cog cov ntaub so ntswg (tshwj xeeb tshaj yog cov nplooj ntoo tshiab) nrog cov ntsiab lus siab polysaccharide thiab polyphenol.
Kev khiav haujlwm
Daim duab
Plant Total RNA Isolation Kit Plus ua tiav 50mg ntawm nplooj tshiab ntawm polysaccharides thiab polyphenols, thiab 5% purified RNA raug kuaj los ntawm electrophoresis.
1: Banana
2: Ginkgo
3: paj rwb
4: Pomegranate
Cia thiab Txee lub neej
Cov khoom siv no tuaj yeem khaws cia rau 24 lub hlis nyob rau hauv qhuav qhuav ntawm chav tsev kub (15-25 ℃);yog tias nws yuav tsum tau khaws cia rau lub sijhawm ntev, nws tuaj yeem khaws cia hauv 2-8 ℃.
Tsis PSL1 tuaj yeem muab tso rau ntawm 4 ℃ rau 1 lub hlis tom qab ntxiv β-mercaptoethanol (nws pom zoo kom ntxiv rau tib lub sijhawm ntawm kev sim).
